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Versatile Transfection Reagent Offers Low Toxicity and Consistent,,,Performance

color="#002DE5"> Reagent

Cell Type

DNA (mg)

LipoTAXI reagent (ml)

SAEC

1.0

5

PrEC

1.5

5

SkMC

1.5

5

HMEC

1.0

10

We included data from optimization studies in several cell lines to illustrate that the cell-line variability of optimization results when using LipoTAXI reagent and DNA.3 Table 1 summarizes the findings of optimization studies for additional cell types from Clonetics Corporation: small airway epithelial cells (SAEC), skeletal muscle cells (SkMC), and human mammary epithelial cells (HMEC). For each cell type, the optimal amount of DNA and volume of LipoTAXI transfection reagent is indicated.

The cell-line variability of transfection efficiency was also demonstrated in another study that compared LipoTAXI reagent to four other lipid transfection reagents in A549, COS-7, and N
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