LipoTAXI transfection reagent: A proven choice
Matt C. Petre
LipoTAXI transfection reagent offers improved, consistent transfections for both adherent cells and cells growing in suspension. In this article, we summarize the results of several experiments that characterize the effectiveness of LipoTAXI reagent. Optimizing transfection conditions is a critical, preliminary step to achieving high transfection efficiencies, a need supported by data showing that transfection efficiencies vary with different cell lines and different lipid-transfection reagents. In addition, we examine the effect of mycoplasma contamination of cell lines on transfection efficiency. Finally, we show that LipoTAXI reagent is superior by comparing it to another lipid-based transfection reagent. Together, these studies confirm the high efficiency and low toxicity of LipoTAXI transfection reagent.
Using transfection procedures to introduce foreign DNA into mammalian cells is integral to many research endeavors. Consequently, focused efforts are directed toward improving transfection technology. Lipid-mediated transfection reagents have improved the technology because they are easy to use, gentle to cells, and yield consistent results.1,2 However, even for lipid-based procedures, a variety of factors can influence transfection efficiency. These include DNA concentration,3 choice and volume of transfection reagent,4 choice of cell line,5 and presence of mycoplasma contamination6.
To achieve high transfection efficiencies, we advise researchers using
Stratagenes LipoTAXI transfection reagent to experimentally determine the
concentration of DNA and volume of LipoTAXI transfection reagent. To optimize
transfection in any cell type, it is standard to measure the signal inten