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Versatile Reporter Vectors for Monitoring Viral Transduction

rmine transduction efficiencies for the vector, each of five target cell lines were infected with serially diluted viral supernatant and, after a 2-day expression period, the transduction efficiency was determined by FACS. Titers were calculated2,3 for supernatant dilutions that fell within the linear dose:response range for transduction. Titers in excess of 107 cfu/ml were achieved for four out of the five cell lines tested (Table 1). For experiments in which cells were infected at a low multiplicity of infection (MOI) and, thus, harbor only single copies of the hrGFP provirus, fluorescence over background was readily detectable with this vector.

Vector

Dilution

CHO

293

HeLa

COS-7

NIH-3T3

pFB-hrGFP
vector

1:103

1.4 x 107

4.9 x 107

2.0 x 107

2.0 x 107

2.5 x 106

1:104

2.0 x 107

6.3 x 107

2.4 x 107


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