( rmine transduction efficienc...)Versatile Reporter Vectors for Monitoring Viral Transduction

rmine transduction efficiencies for the vector, each of five target cell lines were infected with serially diluted viral supernatant and, after a 2-day expression period, the transduction efficiency was determined by FACS. Titers were calculated^2,3 for supernatant dilutions that fell within the linear dose:response range for transduction. Titers in excess of 10⁷ cfu/ml were achieved for four out of the five cell lines tested (Table 1). For experiments in which cells were infected at a low multiplicity of infection (MOI) and, thus, harbor only single copies of the hrGFP provirus, fluorescence over background was readily detectable with this vector.

Table 1
Viral Titers* for pFB-hrGFP and pFB-Neo-LacZ vectors

Vector

Dilution

CHO

293

HeLa

COS-7

NIH-3T3

pFB-hrGFP
vector

1:10³

1.4 x 10⁷

4.9 x 10⁷

2.0 x 10⁷

2.0 x 10⁷

2.5 x 10⁶

1:10⁴

2.0 x 10⁷

6.3 x 10⁷

2.4 x 10⁷

'"/>

Source:


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