Navigation Links
Versatile Reporter Vectors for Monitoring Viral Transduction



Determine retroviral transduction efficiency and gene expression in virtually any target cell line

Brenda Rogers Katherine Felts Peter Vaillancourt
Stratagene

We describe three new ViraPort retroviral reporter vectors: pFB-hrGFP Y, pFB-Neo-LacZ ,, and pFB-Luc , which can be used to determine transduction efficiency and gene expression in virtually any target cell line. To assay for expression, use simple fluorescence microscopy or FACS with pFB-hrGFP, carry out a b-galactosidase-activity assay or in situ staining with pFB-Neo-LacZ, or perform a luciferase activity assay with pFB-Luc. These control vectors are available as plasmid DNA as well as transduction-ready, replication-incompetent, VSV-G pseudotyped high-titer retroviral supernatants. The vectors are particularly useful as controls when using the ViraPort retroviral gene expression systems.

We recently introduced a set of human and rodent ViraPort retroviral cDNA expression libraries inserted into the MMLV-based high-titer retroviral vector pFB1,2. These complex cDNA libraries, part of our growing collection of ViraPort retroviral premade libraries, are easily and efficiently introduced into virtually any mitotic cell type for screening based on gene function. The insert capacity of pFB, our MMLV-based replication-defective pFB vector, is approximately 8.0 kb because there is no extraneous sequence between the LTRs. We excluded selectable markers or readily detectable reporter genes from the ViraPort retroviral gene expression systems to ensure that the largest cDNAs possible could be inserted.

Dispensing with marker g
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. A Versatile Power Supply for All Electrophoresis Applications
2. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
3. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
4. pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA
5. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
6. Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion Transactivators
7. New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways
8. Genetic Reporter Systems
9. Genetic Reporter Systems
10. Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment
11. A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Versatile Reporter Vectors for Monitoring Viral Transduction

(Date:5/27/2015)... NJ (PRWEB) May 27, 2015 The ... evaluating the impact of regulation on medical innovation, has ... Innovation (iPCI) of Fairleigh Dickinson University’s ( FDU ... MI3 Alert pertains to new FDA (Food ... proposal for accelerating the approval of certain medical devices. ...
(Date:5/26/2015)... GERMANY (PRWEB) May 26, 2015 ... R. Grace & Co. (NYSE: GRA) announces that ... good manufacturing practice (GMP) certification from EXCiPACT™, an ... of pharmaceutical excipients. , All three ... of pharmaceutical grade excipient silica gels have now ...
(Date:5/26/2015)... May 26, 2015  Sangamo BioSciences, Inc. (NASDAQ: SGMO ... and CEO, will provide an update on the progress of ... of the company,s business strategy at 1:30pm ET on Monday, ... The conference is being held in New York, ... live and may be accessed via a link on the ...
(Date:5/26/2015)... Global Stem Cells Group and ... company’s first cellular and regenerative medicine symposium in Barcelona ... hands-on stem cell training class Oct. 10. The event ... President of Therapeutic Confrontations (CONFTERA) in Barcelona. , ... regenerative medicine and recent advances in cellular therapies, Global ...
Breaking Biology Technology:Medical Innovation Impact Index Launched at Fairleigh Dickinson University’s Initiative for Patient-Centered Innovation (iPCI) under the Rothman Institute 2CORRECTION: Grace European Facility Receives GMP Excipient Certification for SYLOID® FP Silica Gel 2Sangamo BioSciences Announces Presentation At The Jefferies 2015 Global Healthcare Conference 2Stem Cell Training, Inc. Announces First European Symposium on Cellular and Regenerative Medicine 2Stem Cell Training, Inc. Announces First European Symposium on Cellular and Regenerative Medicine 3
... Agreement on Molecular Tests... -- GERMANTOWN, Maryland and DES PLAINES, Illinois, October 12, 2010, ... ... --> ... s = ,; var first_result; // Results are keyed by longUrl, so we need to grab the ...
... Calif., Oct. 12 WaferGen Biosystems, Inc. (OTC Bulletin ... state-of-the-art genomic analysis systems, today announced that Gary P. ... next generation sequencing for gene expression at Illumina, Inc., ... Research and Applications, a new position created to build ...
... With more than 200 researchers in attendance at ... two technologies that facilitate data-driven research: the Microsoft ... (Logo: http://photos.prnewswire.com/prnh/20000822/MSFTLOGO ) ... developers in bioscience now have access to the ...
Cached Biology Technology:QIAGEN and Abbott Enter Into Agreement on Molecular Tests for HIV, HCV and HPV 2QIAGEN and Abbott Enter Into Agreement on Molecular Tests for HIV, HCV and HPV 3QIAGEN and Abbott Enter Into Agreement on Molecular Tests for HIV, HCV and HPV 4QIAGEN and Abbott Enter Into Agreement on Molecular Tests for HIV, HCV and HPV 5QIAGEN and Abbott Enter Into Agreement on Molecular Tests for HIV, HCV and HPV 6QIAGEN and Abbott Enter Into Agreement on Molecular Tests for HIV, HCV and HPV 7QIAGEN and Abbott Enter Into Agreement on Molecular Tests for HIV, HCV and HPV 8QIAGEN and Abbott Enter Into Agreement on Molecular Tests for HIV, HCV and HPV 9QIAGEN and Abbott Enter Into Agreement on Molecular Tests for HIV, HCV and HPV 10WaferGen Names Gary P. Schroth, Ph.D., as New SVP, Genomics Research and Applications 2WaferGen Names Gary P. Schroth, Ph.D., as New SVP, Genomics Research and Applications 3WaferGen Names Gary P. Schroth, Ph.D., as New SVP, Genomics Research and Applications 4WaferGen Names Gary P. Schroth, Ph.D., as New SVP, Genomics Research and Applications 5Microsoft Research Makes Microsoft Biology Foundation and MODISAzure-Based Environmental Service Available to Scientists and Researchers 2Microsoft Research Makes Microsoft Biology Foundation and MODISAzure-Based Environmental Service Available to Scientists and Researchers 3Microsoft Research Makes Microsoft Biology Foundation and MODISAzure-Based Environmental Service Available to Scientists and Researchers 4
(Date:5/19/2015)... , May 19, 2015  Technology is rapidly advancing, ... and in the cloud. Passwords and their management are ... OTP and standards-based specifications such as those developed by ... use of passwords presents for BYOD, COPE, IoT, and ... biometric identity protocol. In response to ...
(Date:5/14/2015)... , May 14, 2015  Verificient ... identity verification and online remote proctoring, announced ... software-as-a-service (SaaS) company and creator of the ... of the two companies will benefit from ... Canvas. As a fully integrated ...
(Date:5/10/2015)... Fingerprint Cards (FPC) has received an ... the distributor World Peace Industrial Group (WPI), part of WPG ... Asia . Deliveries are planned to take place during ... manufacturers in China . The order value ... of + 1 000 MSEK for 2015.   Jörgen ...
Breaking Biology News(10 mins):HYPR Corp. Biometric Security Integration Kit Shields Applications and Devices Across the Internet of Things 2HYPR Corp. Biometric Security Integration Kit Shields Applications and Devices Across the Internet of Things 3Verificient and Instructure Announce Alliance Partnership to Provide Online Proctoring for K-12 and Higher Education Institutions 2Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 235 Million 2
... press release is available in German . , ... time, show changes in their mechanical properties. The exact course ... the microscopic structure and the complexity of the systems make ... led by Professor Andreas Bausch from the Chair of Cellular ...
... scientists have solved a perplexing mystery regarding one of ... shedding light on a possible route to treatment of ... disease occurs when a genetic mutation causes excessive amounts ... a chain-like molecule the body uses to temporarily store ...
... from a devastating condition known as critical limb ischemia ... amputation. The disease is linked to the blockage ... treatment options include rehabilitative exercise and surgical bypass of ... be a way to restore blood supply in skeletal ...
Cached Biology News:Discovery of source of glycogen 'manufacturing' errors sheds light on fatal disease 2Scientists discover genetic switch that increases muscle blood supply 2Scientists discover genetic switch that increases muscle blood supply 3
...
IMAGEQUANT 300 IQTL, 1 EA. Category: ImageQuant CCD Imagers....
RayBio Human Cytokine Antibody Array 1.1 (4 array membranes) with Accessory, detects 20 cytokines (for lysate) Class: Antibody Array Products Product Group: Antibody Array...
Recombinant Feline IL-12/IL-23 p40...
Biology Products: