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Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)

cause a Group Settings dialog box will appear). Enter a name for the group, (Pipettor#1 in this example) and select Unknowns from the Column Format pop-up menu. For example, you may choose a group name that designates the pipettor and/or dispense volume and assign well names that correspond to the pipettor channels. A sample template for an 8-channel pipettor is shown in Figure 9. Rows A through H correspond to pipettor channels 1 through 8.

For a 12-channel pipettor, you can set columns 1 through 12 to correspond to pipettor channels 1 through 12.

Step 4 Set up the Reduction as described in Step 3 of the preceding section ( Creating Plate sections for the calibration curve , page 3) and illustrated in Figure 2. In the Reduction dialog box, select Custom from the Wavelength Combination pop-up menu, then click on the formula button to display the Calculation dialog box and type !Pathlength in the formula field.

Step 5 Click the Grayscale button in the Plate sections Display dialog box (Figure 3 on page 4). Grayscale display is useful for quick visualization of the plate. Check the With reduced number box, so that the pathlength (in cm) will be displayed in each well. You do not need to enter the low and high values; SOFTmax PRO will automatically assign limits when the plate is read.

Note: To avoid confusion, it is important that the Display is NOT set to display Raw OD as a number. The Raw OD is the OD value at the UV/Vis wavelength that you were required to enter, but that value is ignored for Pathlength calculation.

Step 6 Take a clean microplate from the same lot as that used to prepare the calibration curve. Using the multi-channel pipettor, fill the entire microplate with the chosen dispense volume.

Step 7 Read the plate in t
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