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Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)

Graphing the calibration curve
Step 1 Open the Group section. Double click the Concentration column header and change its name to Volume.

Because you have set the data reduction to pathlength, the Values and MeanValues columns display the pathlength (in centimeters). Change the name of the Values column to Pathlength. Change the Mean-Value column header to MeanPathlength.

An example of a Group Table for a calibration standard curve is shown in Figure 6.


Step 2 Create a new Graph section, then open the Plots dialog box ( Figure 7). In the Plot name: field type STD (note: if you name the plot STD, SOFTmax PRO will automatically refer subsequent unknown samples to the standard curve for interpolation.) Select Volume for the X variable and MeanPathlength for the Y variable. If desired, select StDev for Y-axis error bars.


Click OK to close the dialog box and create the standard curve. An example of a standard curve is shown in Figure 8.


Step 3 Save the data file.


CHECKING MULTICHANNEL PIPETTOR PERFORMANCE
Step 1 Open the calibration curve data file. Use the Save As..... command to create a new file for the current calibration.

Step 2 Create a new Plate section. Set up the Instrument Settings dialog box as described in the preceding section (see Figure 1). Select to perform an endpoint read at any wavelength. (SOFTmax PRO requires that you enter a UV/Vis wavelength at which to read, even though this measurement will not be used in calculating pathlength. Select PathCheck with Water Constant and deselect Pre-Read Plate (pre-reading is unnecessary because the UV/VIS measurement is not used in calculating pathlength).

Step 3 Open the Template Editor, create a new group (doing so will
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