Step 2 Select the first column of wells in the template. Type a sample name into the Sample field (e.g. the intended dispense volume), then type the intended dispense volume into the Volume field. In the example, the group was named BufferA and wells A1-H1 of Plate #1 were named 300ul and assigned a volume of 300 L (Figure 5).
Step 3 Open Plate #2 and change the designated strip to column #2 in the Instrument Setup dialog box. In the Template Editor, assign the wells in the second column to the same Group as in Plate#1, then type in the appropriate sample name and volume. In this example, wells A2 through H2 were assigned to BufferA and named 275ul.
Step 4 Repeat Step 3 for the remaining Plate sections, selecting the appropriate column of wells to be read, assigning them to the same group, then giving them sample names and volumes that correspond to their intended dispense volumes.
Preparing and reading the calibration curve plates
Step 1 Select a clean, dry microplate for the calibration. If the calibration curve uses volumes> 150 L, use a regular 96-well plate. For dispense volumes of 40 L to 150 L, use a Costar half-area plate (to maximize the NIR absorbance values and increase sensitivity.
Step 2 Using the certified pipettor, fill column 1 of the microplate with the first chosen dispense volume. In this example, 300 L volumes were dispensed into column 1 the microplate.
Step 3 Read Plate#1 in the SPECTRAmax PLUS. To avoid significant evaporation error, read the plate within a few minutes of filling the wells.
Step 4 Repeat Steps 2 3 with the remaining columns of the microplate, reading the corresponding Plate section immediately after filling each column.