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Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)

/> Step 3 Click the Reduction button in the Plate Sections tool bar to display the Reduction dialog box (Figure 2 ). Set the Data Mode to Absorbance. Select Custom from the Wavelength Combination pop-up menu, then click the formula button to display the Calculation dialog box. Type !Pathlength in the Formula field of the dialog box, then click the OK button to return to the Reduction dialog box.


Note: the Apply Pathcheck box can be ignored for this application (it refers to pathlength-correction of the UV-Vis wavelength that you had to select in Step 2, but will not be using).

Step 4 Click the Display button in the Plate sections toolbar to bring up the Display dialog box (Figure 3). Click the Number button under the Reduction: Custom heading to set the pathlength data to be displayed as a number. Do not check the Number box under the Raw OD heading.


Note: if the Number button under the Raw OD heading is selected, the data displayed will be the OD values at the UV/Vis wavelength that you selected in Step 2, not pathlength, which could cause confusion.

Step 5 With the first Plate section selected, create additional Plate sections. Because the first Plate section is selected, its Instrument, Reduction and Display settings will be duplicated in each of the new Plate sections. Create one Plate section for each intended dispense volume in your calibration curve (this example uses 10 dispense volumes, and therefore 10 Plate sections).


Defining the strips and templates for the calibration curve Plate sections
Step 1 In Plate#1s Template Editor, select to create a new group (doing so will cause a Group Settings dialog box will appear). Enter a name for the group, (BufferA in this example) and select Standards from the Column Format pop-up menu. Type Volume into the Sample Descri
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