Navigation Links
Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)

e, protein or chromogen) to water will lower the surface tension and tend to increase the dispensed volume.1 Thus the volumes of reagents dispensed during routine laboratory procedures may differ from the dispense volume of pure water.

Ideally, pipettor performance should be checked using the actual dispense reagent as the calibrator solution. However, most dispense reagents are colorless and an absorbance measurement cannot be obtained in the visible range (400750 nm) without the addition of a dye. The SPECTRAmax PLUS microplate spectrophotometer overcomes this limitation by using the near infra-red absorbance of water to determine the pathlength in each well, which is proportional to volume. The dispensed volumes are calculated by reference to a pathlength/volume standard curve (prepared separately with a certified pipettor). The standard curve can be prepared once for a given dispense reagent and batch of microplates and stored for subsequent use.

This application note describes how to check the performance of multichannel pipettors with the SPECTRAmax PLUS. Pathlengths in the wells are measured directly and volumes are calculated automatically. The performance can be checked using the same buffers or reagents that are being dispensed by the pipettor, eliminating possible discrepancies in pipettor performance between normal dispense reagent and calibrator solution. Because initial separate calibration of one channel is not necessary (e.g., as in reference 10), the method is suitable for checking performance of automated pipetting stations, including 96channel pipettors. The spreadsheet capability of SOFTmax PRO software allows the data to be calculated automatically and final results to be displayed as overall Pass/Fail for each pipettor.


MATERIALS
1.
SPECTRAmax PLUS microplate spectrophotometer

2. High-quality microplates; e.g.:
For dispense volumes >150 L

'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Related biology technology :

1. Multichannel Pipetting: How to Choose the Correct Pipettor
2. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
3. Comparing Fidelity and Performance of Proofreading PCR Enzymes
4. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
5. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
6. Performance Comparisons of Commercial RT-PCR Systems
7. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
8. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
11. Highly Sensitive microRNA Array Performance
Post Your Comments:
(Date:8/22/2014)... 22, 2014 Today the Academy of Model ... States Court of Appeals for the D.C. Circuit challenging the ... in the Federal Register on June 25, 2014. , ... to the text and intent of the FAA Modernization and ... specific prohibition on any new rules or regulations regarding model ...
(Date:8/22/2014)... at the University of Surrey have discovered how the ... body,s clock genes. , The new study, published today ... this clock activity in turn regulates the cycle of ... also discovered that the local biological clock and its ... of the clock in bladder physiology and ageing. ...
(Date:8/22/2014)... first direct observations of how facets form and ... more sophisticated and effective nanocrystal design and reveal ... crystal growth breaks down at the nanoscale. , ... Lawrence Berkeley National Laboratory (Berkeley Lab) used highly ... fast-detection camera to capture the physical mechanisms ...
(Date:8/22/2014)... 2014 Ophthalmic drug delivery technologies ... challenging endeavors as the anatomy, physiology and biochemistry ... the foreign substances. Ocular drug delivery technologies provide ... and minimal use of needles; also it enables ... provides specific targeting within the ocular globe. On ...
Breaking Biology Technology:AMA Files Petition for Review of FAA’s Interpretive Rule 2Treatment for overactive bladder and irritable bowel syndrome advanced through pioneering research 2Shaping the future of nanocrystals 2Shaping the future of nanocrystals 3Shaping the future of nanocrystals 4Ocular Drug Delivery Technologies Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 2Ocular Drug Delivery Technologies Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 3Ocular Drug Delivery Technologies Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 4
... Inc. (Sparta),the maker of TrackWise(R), and the market ... that its TrackWise solution has,been chosen by Protherics ... manufacturing and marketing of specialized products for,critical care ... handling,processes. TrackWise provides organizations with a single, ...
... Oct. 24 Human Genome,Sciences, Inc. (Nasdaq: HGSI ... to discuss its financial results for the quarter ended,September ... on Thursday,November 1, 2007. (Logo: http://www.newscom.com/cgi-bin/prnh/20010612/HGSLOGO ) ... management and will be,held on Thursday, November 1, 2007, ...
... Call on Thursday, October 25, 2007 at ... ... Keryx Biopharmaceuticals,Inc. (Nasdaq: KERX ), a biopharmaceutical company focused ... the treatment of life-threatening,diseases, including diabetes and cancer, today announced its ...
Cached Biology Technology:Protherics UK Ltd. Chooses TrackWise for Corrective Action and Preventive Action (CAPA) Management 2Human Genome Sciences To Sponsor Conference Call To Discuss Third Quarter 2007 Financial Results 2Keryx Biopharmaceuticals, Inc. Announces Third Quarter 2007 Financial Results 2Keryx Biopharmaceuticals, Inc. Announces Third Quarter 2007 Financial Results 3Keryx Biopharmaceuticals, Inc. Announces Third Quarter 2007 Financial Results 4Keryx Biopharmaceuticals, Inc. Announces Third Quarter 2007 Financial Results 5Keryx Biopharmaceuticals, Inc. Announces Third Quarter 2007 Financial Results 6
(Date:8/21/2014)... Aug. 21, 2014 Nxt-ID, Inc. (OTCQB: NXTD), ... commerce market, announced today that its shares of common ... be issued in its proposed underwritten public offering of ... on The Nasdaq Capital Market, subject to closing of ... and warrants, and will trade under the symbol "NXTD" ...
(Date:8/21/2014)... , Aug. 21, 2014 /PRNewswire-iReach/ -- LiveScan ... has agreed to a partnership with Gabriel Health ... fastest growing Certified Nursing Assistant preparatory ... Institute  an exclusive member of Binary,s LiveScan service ... Biometrics will improve its service capabilities by ...
(Date:8/21/2014)... the Fram Strait is a key region in the global ... Greenland and Svalbard warm Atlantic water flows to the north ... Arctic water masses and sea ice push their way out ... of the Atlantic water cools here on its way to ... the water caused in this manner drives the global band ...
Breaking Biology News(10 mins):Nxt-ID Approved for NASDAQ Capital Market Listing 2Nxt-ID Approved for NASDAQ Capital Market Listing 3LiveScan Provider Binary Biometrics Announces Partnership with Preeminent CNA School, Gabriel Health Institute to Expand Services in Orlando Region 2LiveScan Provider Binary Biometrics Announces Partnership with Preeminent CNA School, Gabriel Health Institute to Expand Services in Orlando Region 3Arctic sea ice influenced force of the Gulf Stream 2Arctic sea ice influenced force of the Gulf Stream 3
... MADISON Times are tough for wildlife living at the ... Armies are reportedly encamped in a national park and wildlife ... cattle have settled in an adjoining Ugandan park. And ... support more than 900 individuals of the emblematic African lion, ...
... Clinical Research Services (TCRS) at Scottsdale Healthcare and Mayo ... broad potential to treat solid tumors. Clinical ... TGen Clinical Research Services at Scottsdale Healthcare, a partnership ... Scottsdale-based Scottsdale Healthcare Corp., and at Mayo Clinic in ...
... AWRE ), a leading supplier of broadband technology ... the company,s operating results for the fourth quarter and fiscal ... Eastern Time. CEO Michael Tzannes and CFO Rick Moberg will ... Thomson and can be accessed on the Investor Relations page ...
Cached Biology News:Research uncovers surprising lion stronghold in war-torn central Africa 2Research uncovers surprising lion stronghold in war-torn central Africa 3TGen Clinical Research Services at Scottsdale Healthcare and Mayo Clinic study new cancer drug 2Aware Announces Q4 and FY2008 Earnings Conference Call 2
Recognizes NSCL1. The molecular weight of the protein is 14,616 Daltons....
FEN-1 (B-4)...
Secure-Seal™ spacer, one well, 20 mm diameter, 0.12 mm deep *set of 100*...
Mouse monoclonal [2D7/1] to E.coli LPS ( Abpromise for all tested applications). Antigen: Tissue / cell preparation: Escherichia coli J5 cells....
Biology Products: