Navigation Links
Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)

en calculated from the absorbance, the light path, the extinction coefficient and the diluent volume. Various chromogens have been used, including Ponceau S dye, methyl red, Evans Blue dye and nicotinamide.1 ,2,10,11 The College of American Pathologists and the NCCLS recommend potassium dichromate because it can be obtained as a primary standard.1,2

The NCCLS and the ASTM recommend that for every pipettor, 10-replicate tests for calibration verification, measuring both inaccuracy and imprecision, be performed at least quarterly and after any maintenance.7,9 For the monthly quick check they recommend 4-replicate tests. If a laboratory has only a few single-channel pipettors, these guidelines can be reasonably accommodated using the gravimetric method. However, in laboratories having numerous pipettors, including multi-channel devices and automated pipetting stations, the testing requirement becomes extremely tedious at best, and impractical at worst. A much more practical approach for multichannel pipettors would be a spectrophotometric procedure in a 96-well format such as that suggested by Kaufman and Wobig.10 The delivery volume of one channel is checked gravimetrically, and the calibration adjusted if necessary. The remaining channels are then compared to the calibrated channel by dispensing a dye solution into the wells of a 96-well plate and measuring the absorbance in a microplate reader. Accuracy is calculated relative to the first channel with a separately-determined calibration factor of absorbance change per microliter.

An important (though often ignored) consideration in pipettor calibration is the fact that variations in surface tension, viscosity, density and temperature of the dispense reagent will lead to differences in dispensed volumes.1,4,7,8,12,13 Addition of a solute (whether buffer, preservativ
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Related biology technology :

1. Multichannel Pipetting: How to Choose the Correct Pipettor
2. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
3. Comparing Fidelity and Performance of Proofreading PCR Enzymes
4. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
5. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
6. Performance Comparisons of Commercial RT-PCR Systems
7. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
8. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
11. Highly Sensitive microRNA Array Performance
Post Your Comments:
(Date:8/22/2014)... the University of Surrey have discovered how the receptors ... clock genes. , The new study, published today in ... clock activity in turn regulates the cycle of all ... discovered that the local biological clock and its control ... the clock in bladder physiology and ageing. ...
(Date:8/22/2014)... are intensively investigated for application as catalysts, ... and components in photonics and molecular electronics. ... for understanding physical and chemical properties, has ... USA, have demonstrated that high-resolution electron microscopy ... structure in which all gold atoms are ...
(Date:8/22/2014)... Research and Markets has announced ... Permeation Chromatography (GPC) Industry Report 2014" report to ... Gel Permeation Chromatography (GPC) Industry Report is a professional ... global gel permeation chromatography industry with a focus on ... overview of the industry including definitions, classifications, applications and ...
(Date:8/22/2014)... 2014 Nerium International, the go-to, ... the spotlight again. Nerium will showcase its signature ... Body Contouring Cream to Hollywood’s elite at the ... the 66th Primetime Emmy® Awards. , Developed from ... clinical trials show that NeriumAD night cream dramatically ...
Breaking Biology Technology:Treatment for overactive bladder and irritable bowel syndrome advanced through pioneering research 2A breakthrough in imaging gold nanoparticles to atomic resolution by electron microscopy 2Global and China Gel Permeation Chromatography (GPC) Industry Report 2014 2Nerium International Joins Television’s Biggest Stars at Backstage Creations Giving Suite at the 66th Primetime Emmy Awards 2Nerium International Joins Television’s Biggest Stars at Backstage Creations Giving Suite at the 66th Primetime Emmy Awards 3
... Nectid, Inc. ( www.nectid.com ) announced today that ... patents and patents-applications to Grunenthal GmbH ( www.grunenthal.com ), ... in pain drug discovery and research. The patent portfolio ... Pain Drug Discovery program and two key drug delivery ...
... , COLUMBUS, Ohio Engineers at Ohio State University have ... from nine different angles at once to create a 3D ... that move around an object; the new lens is the ... itself. Allen Yi, associate professor of integrated systems engineering ...
... NEW YORK, March 21, 2011 Reportlinker.com announces ... available in its catalogue: ... http://www.reportlinker.com/p0459142/Global-Angiongenesis-Inhibitors-and-Stimulators-Industry.html ... Angiogenesis Inhibitors and Stimulators in US$ Million ...
Cached Biology Technology:Seeing in stereo: Engineers invent lens for 3-D microscope 2Reportlinker Adds Global Angiongenesis Inhibitors and Stimulators Industry 2Reportlinker Adds Global Angiongenesis Inhibitors and Stimulators Industry 3Reportlinker Adds Global Angiongenesis Inhibitors and Stimulators Industry 4Reportlinker Adds Global Angiongenesis Inhibitors and Stimulators Industry 5Reportlinker Adds Global Angiongenesis Inhibitors and Stimulators Industry 6Reportlinker Adds Global Angiongenesis Inhibitors and Stimulators Industry 7Reportlinker Adds Global Angiongenesis Inhibitors and Stimulators Industry 8Reportlinker Adds Global Angiongenesis Inhibitors and Stimulators Industry 9Reportlinker Adds Global Angiongenesis Inhibitors and Stimulators Industry 10Reportlinker Adds Global Angiongenesis Inhibitors and Stimulators Industry 11Reportlinker Adds Global Angiongenesis Inhibitors and Stimulators Industry 12Reportlinker Adds Global Angiongenesis Inhibitors and Stimulators Industry 13Reportlinker Adds Global Angiongenesis Inhibitors and Stimulators Industry 14Reportlinker Adds Global Angiongenesis Inhibitors and Stimulators Industry 15Reportlinker Adds Global Angiongenesis Inhibitors and Stimulators Industry 16Reportlinker Adds Global Angiongenesis Inhibitors and Stimulators Industry 17
(Date:8/22/2014)... German . ... are fixated in a way so as to align ... and Fudan University researchers are planning on looking into ... findings are being published today in the scientific journal ... sensitive molecules. Everyone knows that at least from ...
(Date:8/22/2014)... strong antioxidants and can reduce free radical ... against oxidative stress? Jianbo Zhao and co-workers ... Medical University, China discovered that green tea ... neuronal apoptosis, indicating green tea polyphenols play ... under oxidative stress. The relevant study has ...
(Date:8/22/2014)... In 2015, American consumers will finally be able to ... Although touted as zero-emissions vehicles, most of the cars ... fossil fuel that contributes to global warming. , Now ... device that uses an ordinary AAA battery to produce ... electric current through two electrodes that split liquid water ...
Breaking Biology News(10 mins):Proteins: New class of materials discovered 2Stanford scientists develop a water splitter that runs on an ordinary AAA battery 2Stanford scientists develop a water splitter that runs on an ordinary AAA battery 3
... human genetics at Baylor College of Medicine, first published her ... starvation, sometimes resulting in a rare change that benefited the ... current issue of the journal Molecular Cell, she and her ... that this only occurs at a special time and place ...
... prediction. , Computers can predict the detailed structure of ... least some of the time, according to new studies ... which were reported in the September 16, 2005, issue ... that scientists eventually may be able to determine the ...
... led by University of California, San Diego biochemists has ... produce 10 trillion varieties of a single protein, a ... drugs. , In the September 18 advance on-line publication ... researchers describe the mechanism by which a virus that ...
Cached Biology News:Stressed cells spark DNA repair missteps and speed evolution 2Computers close in on protein structure prediction 2Computers close in on protein structure prediction 3Computers close in on protein structure prediction 4UCSD discovery may provide novel method to generate medically useful proteins 2UCSD discovery may provide novel method to generate medically useful proteins 3
...
Improve inventory management with stainless steel red cell canisters; ensure product safety with CO2 or LN2 backup systems. A complete solution for your blood banking needs....
... oligonucleotides: PTOs are internucleotide modifications, ... backbone is replaced by a sulphur atom. ... degradation. PTOs are available as ... Oligonucleotides with thio-caps ...
Improve inventory management with stainless steel red cell canisters; ensure product safety with CO2 or LN2 backup systems. A complete solution for your blood banking needs....
Biology Products: