Navigation Links
Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)

en calculated from the absorbance, the light path, the extinction coefficient and the diluent volume. Various chromogens have been used, including Ponceau S dye, methyl red, Evans Blue dye and nicotinamide.1 ,2,10,11 The College of American Pathologists and the NCCLS recommend potassium dichromate because it can be obtained as a primary standard.1,2

The NCCLS and the ASTM recommend that for every pipettor, 10-replicate tests for calibration verification, measuring both inaccuracy and imprecision, be performed at least quarterly and after any maintenance.7,9 For the monthly quick check they recommend 4-replicate tests. If a laboratory has only a few single-channel pipettors, these guidelines can be reasonably accommodated using the gravimetric method. However, in laboratories having numerous pipettors, including multi-channel devices and automated pipetting stations, the testing requirement becomes extremely tedious at best, and impractical at worst. A much more practical approach for multichannel pipettors would be a spectrophotometric procedure in a 96-well format such as that suggested by Kaufman and Wobig.10 The delivery volume of one channel is checked gravimetrically, and the calibration adjusted if necessary. The remaining channels are then compared to the calibrated channel by dispensing a dye solution into the wells of a 96-well plate and measuring the absorbance in a microplate reader. Accuracy is calculated relative to the first channel with a separately-determined calibration factor of absorbance change per microliter.

An important (though often ignored) consideration in pipettor calibration is the fact that variations in surface tension, viscosity, density and temperature of the dispense reagent will lead to differences in dispensed volumes.1,4,7,8,12,13 Addition of a solute (whether buffer, preservativ
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Related biology technology :

1. Multichannel Pipetting: How to Choose the Correct Pipettor
2. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
3. Comparing Fidelity and Performance of Proofreading PCR Enzymes
4. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
5. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
6. Performance Comparisons of Commercial RT-PCR Systems
7. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
8. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
11. Highly Sensitive microRNA Array Performance
Post Your Comments:
(Date:12/15/2014)... NEW YORK & PETACH TIKVAH, ... Therapeutics Inc. (NASDAQ: BCLI ), a leading ... announced today that wholly-owned subsidiary Brainstorm Cell Therapeutics Ltd. ... Israel,s Office of the Chief ... has been in receipt of grant support from the ...
(Date:12/13/2014)... CanDiag, Inc., a University of North Carolina at ... to OncoTab, Inc. , “The new name is ... said CEO and Chief Science Officer, Dr. Pinku Mukherjee, ... diagnostics to include imaging and therapies, and our new ... in 2011 by Dr. Mukherjee, an Irwin Belk Endowed ...
(Date:12/13/2014)... 2014 The “Global Synthetic Biology ... drivers, threats, opportunities, and challenges. , The global ... players, namely, Thermo Fisher Scientific, Inc. (U.S.), DuPont ... jointly accounted for approximately ~65% of the total ... Copy for This Report @ http://www.marketsandmarkets.com/Market-Reports/synthetic-biology-market-889.html ...
(Date:12/13/2014)... , Dec. 12, 2014  Vaccinex Inc., ... discovery and development of therapeutic monoclonal antibodies ... announced publication of a manuscript entitled "SEMA4D ... remyelination in neurodegenerative disease" in the January ... Disease*.  The publication highlights an important role ...
Breaking Biology Technology:BrainStorm Receives $1,100,000 for its 8th Annual Non-Dilutive Grant from Israel's Chief Scientist Office 2BrainStorm Receives $1,100,000 for its 8th Annual Non-Dilutive Grant from Israel's Chief Scientist Office 3CanDiag Inc. Expands Product Scope Under a New Name: OncoTab, Inc. 2Synthetic Biology Market to HIt $5.6304 Billion by 2018 - New Research Report by MarketsandMakets 2Synthetic Biology Market to HIt $5.6304 Billion by 2018 - New Research Report by MarketsandMakets 3Vaccinex announces publication of preclinical studies with an anti-SEMA4D antibody in animal models of neuroinflammatory and demyelinating disease 2
... University of California, San Diego electrical engineers are building a ... solar energy without using fossil fuels and harvest it for ... the team said nanowires, which are made from abundant natural ... way to deliver hydrogen fuel on a mass scale. ...
... March 7, 2012 Neuralstem, Inc. (NYSE Amex: ... cells in the cervical (upper back) region of the spine ... trial of its spinal cord neural stem cells in amyotrophic ... also the first woman to be treated in the trial. ...
...   Camelot Healthcare Training Institute , a post-secondary nursing ... has been acquired by Medtech College.  Camelot now joins ... College once appropriate state approvals have been obtained. ... campuses offering accredited training in nursing and a number ...
Cached Biology Technology:Nanotrees harvest the sun's energy to turn water into hydrogen fuel 2Fourteenth Patient Dosed in Neuralstem ALS Stem Cell Trial 2Fourteenth Patient Dosed in Neuralstem ALS Stem Cell Trial 3Camelot Healthcare Training Institute Acquired by Medtech 2Camelot Healthcare Training Institute Acquired by Medtech 3
(Date:12/3/2014)... Dec. 2, 2014   Marvin Test Solutions ... test solutions for military, aerospace, and manufacturing organizations, ... its successful TS-900 PXI semiconductor test platform ... of high-end systems to customers at a fraction ... to traditional ATE. "Our semiconductor ...
(Date:11/21/2014)... 2014 C-Labs LLC, a leading provider of ... (IoT), today announced the appointment of John Traynor ... a strategic advisor to the firm, Mr. Traynor will ... is based out of the C-Labs office in ... Chris Muench , Chief Executive Officer. Photo ...
(Date:11/18/2014)... 2014   EyeLock Inc. , a market ... MorphoTrust USA (Safran), a leading U.S. provider ... strategic partnership to offer comprehensive biometric identity solutions ... airport screening and financial services markets. ... consumers through a nationwide network of 1,100 IdentoGO® ...
Breaking Biology News(10 mins):Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 2Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 3C-Labs Names Former Microsoft and Bsquare Executive as Chief Operating Officer 2EyeLock, MorphoTrust Form Strategic Partnership to Pursue Commercial Enterprise Deployments 2EyeLock, MorphoTrust Form Strategic Partnership to Pursue Commercial Enterprise Deployments 3
... University of Birmingham have unlocked one of the mysteries of ... Resonance Imaging (fMRI) and electroencephalography (EEG). The work will ... up to now could not be studied, allowing a more ... Functional MRI is commonly used to study how ...
... Biological Chemistry identifies a new therapeutic approach to treat ... the research shows that expression of the FoxM1 protein is ... study appears in the journal,s Aug. 2 edition. ... prostate epithelial cells inhibits tumor cell proliferation, the process by ...
... 2013 Breath analysis may prove to be an ... bacterial and other infections, according to a UC Irvine ... PLOS ONE . Employing a chemical analysis method ... chemists were able to correlate inflammation levels in laboratory ...
Cached Biology News:A new tool for brain research 2Breath analysis reliably indicates presence, level of infection in mice, UCI study finds 2
One bottle containing 30 ml of protein solution and 0.1% sodium azide....
...
DONOR BOVINE SERUM AND PLASMA...
Full-length cDNA 5-PRIME end of clone CS0DE013YM09 of Placenta of Homo sapiens (human). [Source:Uniprot/SPTREMBL;Acc:Q86TS7] Antigen: Recombinant Protein Epitope Signature Tag (PrEST)....
Biology Products: