Navigation Links
Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)

f each channel gravimetrically.

2 Reliability. The performance check can be done using the same buffers or reagents that will be dispensed by the pipettor, thus eliminating possible discrepancies in between pipettor performance with calibrator solution and with normal dispense reagent.

In the example presented above, the mean dispense volumes for 2 of the 8 channels fell outside the acceptable range (198 - 202 L). Both mean values are significantly different from the nominal value of 200 L (P<.05, students t-test), however, neither is significantly different from the lower limit (198 L). Thus the channels are probably out of specification, but the ultimate Pass/Fail decision depends upon the laboratorys approach to taking measurement uncertainty into account. In a recent publication,14 Ellison et al nicely summarize and interpret the International Standards Organizations recommended method for quantitative estimation of uncertainty.15 The paper also stresses the importance of correct interpretation of accuracy results, so that they are judged neither overly optimistically, nor unduly pessimistically. In practice, such statistical analyses can be avoided by merely repeating the calibration measurements to ensure that the calibration is within or outside the acceptable range.

The terms accurate and precise are relative. A 5% error may be considered insignificant in one method and totally unacceptable in another. Pipettor manufacturers specifications for dispense volumes of 100 - 200 L are typically less than 1% for both accuracy (+ %) and imprecision (CV%), if obtained under nearly ideal conditions. For clinical analyses, medically-allowable errors (of which pipetting error would be only one of several potential contributors) can be 5%, 10% or 20%.16 Thus many clinical laboratories accept an accuracy requirement of + 3% and a precision requirement of + 1.5% for pipet dispense volum
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Related biology technology :

1. Multichannel Pipetting: How to Choose the Correct Pipettor
2. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
3. Comparing Fidelity and Performance of Proofreading PCR Enzymes
4. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
5. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
6. Performance Comparisons of Commercial RT-PCR Systems
7. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
8. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
11. Highly Sensitive microRNA Array Performance
Post Your Comments:
(Date:5/22/2013)...  Empire Genomics announced today it has acquired ... genomic biomarker from Cornell University for use in ... in diagnosing and determining treatment for patients with ... prostate cancer (NEPC) is a lethal variant of ... but more commonly arises after hormonal therapy for ...
(Date:5/22/2013)... , May 22, 2013  Superior Controls, Inc. ... 2013" by Business NH Magazine.  For the past ... organizations that distinguish themselves with extraordinary business and ... to receive this honor," said Rick ... with any recognition, this award is a reflection ...
(Date:5/22/2013)... Mass. and NEW ORLEANS ... leader in the field of regenerative medicine, presented new ... economists from Analysis Group (a leading health economics consultancy) ... diabetic foot ulcers (DFU) and venous leg ulcers (VLU) ... The results of this research, which reveal annual costs ...
(Date:5/22/2013)... May 22, 2013 Genedata, a ... biotechnology, drug discovery, and life science research, today ... ™ as its bioinformatics platform for strain ... Genedata will provide AB Enzymes, one of the ... hosted solution of Genedata Selector accompanied by bioinformatics ...
Breaking Biology Technology:Empire Genomics Licenses Novel DNA Biomarker for Use in Diagnosing and Creating a Companion Diagnostic Test for Neuroendocrine Prostate Cancer 2Superior Controls of Seabrook, NH named Business of the Year for 2013 by Business NH Magazine 2Organogenesis Presents Findings from Groundbreaking Research Highlighting the Healthcare Resource and Economic Burden of Venous Leg Ulcers and Diabetic Foot Ulcers 2Organogenesis Presents Findings from Groundbreaking Research Highlighting the Healthcare Resource and Economic Burden of Venous Leg Ulcers and Diabetic Foot Ulcers 3Organogenesis Presents Findings from Groundbreaking Research Highlighting the Healthcare Resource and Economic Burden of Venous Leg Ulcers and Diabetic Foot Ulcers 4AB Enzymes Chooses Genedata Selector for Production Strain Development and Optimization 2AB Enzymes Chooses Genedata Selector for Production Strain Development and Optimization 3AB Enzymes Chooses Genedata Selector for Production Strain Development and Optimization 4
... "Most Innovative" award by the Association of ... Chinese Canadian Entrepreneurs, ... a biotechnology company discovering and developing the next wave of,antibody ... Executive Officer, received the "Most Innovative" award at the,Chinese Canadian ...
... chemotherapy would welcome an advance promising to dramatically reduce ... researchers at Washington University School of Medicine in St. ... , The researchers focused a powerful drug directly on ... a drug dose 1,000 times lower than used previously ...
... EntreMed, Inc.,(Nasdaq: ENMD ), a clinical-stage ... and inflammatory diseases, today,announced that on April 4, ... Qualifications Department of the NASDAQ Stock Market, LLC. ... "Minimum Bid,Price Requirement," stated that the Company,s common ...
Cached Biology Technology:ARIUS recognized for entrepreneurial excellence 2Nanosized technology has supersized effect on tumors 2Nanosized technology has supersized effect on tumors 3EntreMed Receives Nasdaq Deficiency Notice 2EntreMed Receives Nasdaq Deficiency Notice 3EntreMed Receives Nasdaq Deficiency Notice 4
(Date:5/22/2013)... life scientists provide important new details on how climate ... online May 21 in the Journal of Animal ... making accurate predictions and informing policymakers of how species ... , "There is a growing recognition among biologists ... one another, and that this is going to have ...
(Date:5/22/2013)... 2013 Early screening for prostate cancer could become ... for women, thanks to UC Irvine research published today ... . , After more than a decade of work, ... identify clinically usable markers for prostate cancer in urine, ... with greater accuracy and at dramatically lower cost. The ...
(Date:5/22/2013)... pollinating insects and wild plants have slowed in recent ... by the University of Leeds and the Naturalis Biodiversity ... reductions in the diversity of species in Britain, Belgium ... But the picture brightened markedly after 1990, with a ... hoverflies and wild plants. , Professor Bill ...
Breaking Biology News(10 mins):UCLA life scientists present new insights on climate change and species interactions 2UCLA life scientists present new insights on climate change and species interactions 3UCLA life scientists present new insights on climate change and species interactions 4UCLA life scientists present new insights on climate change and species interactions 5UCI chemists devise inexpensive, accurate way to detect prostate cancer 2UCI chemists devise inexpensive, accurate way to detect prostate cancer 3Encouraging signs for bee biodiversity 2Encouraging signs for bee biodiversity 3
... used a fluorescent marker to predict the individual ... engineered to illuminate stress levels, implying living organisms ... to deal with the rigors of life. , ... genetically identical nematodes were engineered with a green ...
... in the June 20 issue of The Journal of ... led by Colin Tinsley may have discovered how a ... meningitis. , The human body harbors many harmless bacterial ... meningitidis, makes its home in the upper respiratory tract, ...
... significant new advances in determining the structure of all ... takes up where Watson and Crick left off, after ... blueprint for life. , One of the fundamental problems ... molecule from its sequence ?this has been referred to ...
Cached Biology News:Fluorescing lab worms signal longer life spans 2Fluorescing lab worms signal longer life spans 3Major advance made on DNA structure 2Major advance made on DNA structure 3
...
The PROTEAN II slab gel casting stand is a component of the PROTEAN II XL and xi casting apparatus. It is supplied with gaskets....
BD Falcon insert HTS 96 well, square A-bottom with lid. PET membrane, sterile. (5/ca) Packaging: 5 / pack, 5 / case ...
... pLivSelect is a direct antibiotic-based selection system ... on colony survival, eliminating the need for ... process provides close to 100% accuracy in ... platform for robot colony picking. This cloning ...
Biology Products: