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Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)

d in the Notes section report were accessed from the plate and refer to the time and date the plate was read. In this particular example, the Plate section was Plate#12, so the formula for the summary is: !TimeOfRead@Plate#12. Once a plate is read, the Time/Data stamp cannot be changed (unless the data are deleted and new data acquired).

NOTE: you can report results for each individual channel in the final Summary section, however the custom formulas needed to do so are beyond the scope of this application note. For further information on how to set up reports for individual channels, contact Molecular Devices MAXline Technical Support.


DISCUSSION AND SUMMARY
Most pipettors, given enough use, will eventually require repair, and therefore should be checked regularly to avoid having a defective pipettor in service. In the days when the typical laboratory had only a few single-channel, hand-held pipettors, the traditional gravimetric method for calibration was perfectly adequate to satisfy a requirement of 4 to 10 replicates. Modern laboratories, however, have numerous pipettors and many have several multi-channel pipettors and automated pipetting stations. In such cases, the gravimetric method is tedious or even impractical. Pathlength measurements in a 96-well microplate offer an easy and practical alternative method. The major advantages of using pathlength measurements to calibrate or verify performance of multichannel pipettors include:

1 Efficiency. Multiple measurements can be obtained simultaneously, thus the required replicate measurements can be obtained for each channel much more quickly than by making gravimetric measurements. Pathlength measurements for an entire 96-well microplate can be made in less than a minute. The method is especially applicable to automated liquid handling stations (including 96-channel pipettors), where it is impractical to check the performance o
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