Navigation Links
Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells

Key words: SPA Imaging Beads • LEADSeeker • cAMP • receptor binding assay


SPA Imaging Assays are homogeneous immunoassays based on the coupling of one assay component to an SPA Imaging Bead in order to generate a signal. Polystyrene (PS) SPA Imaging Beads coated with donkey anti-rabbit antibody enable the immobilization of rabbit secondary antibodies during the assay. The Donkey Anti-Rabbit PS SPA Imaging Beads bind to the Fc region of the secondary antibody, leaving the antigen binding Fab region unhindered. Application data is demonstrated here for the utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in a cAMP assay.


SPA Immunoassay principle
Binding radiolabeled ligands to antibodies immobilized on the surface of an SPA Imaging Bead brings the radiolabel into close proximity with scintillant incorporated within the bead. The emitted radiation (beta particles for tritium or Auger electrons for iodine-125) then stimulates the scintillant to emit light (Fig 1). Unbound radiolabeled ligand is not in close enough proximity to the scintillant for energy transfer to occur and no signal is generated. Light emitted by stimulated SPA Imaging Beads can be detected by platforms such as the LEADseeker™ Multimodality Imaging System.


cAMP assay
Adrenergic receptors are linked to adenylate cyclase via either inhibitory or stimulatory G proteins. These receptors, when activated by agonist or antagonist occupancy of ligand binding sites, affect the intracellular generation of cAMP. In response to receptor binding, adenylate cyclase converts AMP to cAMP, which exerts its effect by activating a protein kinase capable of phosphorylating specific substrates.

Here, we describe a direct screening 384-well assay that enables the direct extraction and assay of cAMP from c
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
3. Imaging Chemiluminescent Western Blots
4. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
5. Imaging and Analysis of DIGE Product Information Sheet, Rev B
6. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
7. Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
8. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
9. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
10. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
11. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System

Post Your Comments:
(Date:11/25/2009)... to Speak at Economic Club of Canada , ... 2009 -- Ambassador to Speak at Economic Club of C...or for International Investment, and Chairman and ...raphy of David Appia go to www.investinfrance.org...eet with press to discuss recent pro-business init...
(Date:11/25/2009)...Newswire/ - Cytochroma today announced the appoint.... Dr. Moldt will replace Mr. Ulrik Spork as the No...number of Board members remains unchanged at six m...r Moldt to the board and look forward to benefitin...ontinues to make progress in becoming a leading CK...
(Date:11/25/2009)... Reportlinker.com announces that a ...alogue: ,, Brazil Bioethanol Market Analysis a...er.com/p0165503/Brazil-Bioethanol-Market-Analysis-...l Market Analysis and Forecasts to 2013 ,, Sum...and forecasts of the global bioethanol market and ...
(Date:11/24/2009)...man . , Whether it,s CD packaging, container...ncy Identification) are increasingly finding their...label objects or goods and identify them automatic...an read and process the data contained in the labe...uction conditions of up to 100 degrees Celsius. Bu...
Breaking Biology Technology:French Ambassador for International Investment to Visit Toronto December 11 2Cytochroma Announces Appointment of Peter Moldt to Board of Directors 2Reportlinker Adds Brazil Bioethanol Market Analysis and Forecasts to 2013 2Reportlinker Adds Brazil Bioethanol Market Analysis and Forecasts to 2013 3Reportlinker Adds Brazil Bioethanol Market Analysis and Forecasts to 2013 4Reportlinker Adds Brazil Bioethanol Market Analysis and Forecasts to 2013 5Reportlinker Adds Brazil Bioethanol Market Analysis and Forecasts to 2013 6Reportlinker Adds Brazil Bioethanol Market Analysis and Forecasts to 2013 7Reportlinker Adds Brazil Bioethanol Market Analysis and Forecasts to 2013 8Reportlinker Adds Brazil Bioethanol Market Analysis and Forecasts to 2013 9Reportlinker Adds Brazil Bioethanol Market Analysis and Forecasts to 2013 10Intelligence inside metal components 2
...DGE, N.J., Oct. 5 VioQuest,Pharmaceuticals (OTC B...ceutical company focused on acquiring, developing ...announced today that it will,present at the BIO In...p.m.,Pacific Time at the Palace Hotel in the Sea C...uest,s president and CEO, is scheduled to give an,...
...IEW, Calif., Oct. 5 MAP,Pharmaceuticals, Inc. ann...,offering of 5,000,000 shares of common stock at a...ffered by MAP Pharmaceuticals. In addition,MAP Pha... option to,purchase up to an additional 750,000 sh...ch & Co. and Morgan Stanley & Co. Incorporated are...
...EETING, Pa., Oct. 5 Genaera,Corporation (Nasdaq: ...resenting at two upcoming conferences, Biotech 200... InvestorForum in San Francisco on October,9-11, 2...ent and Chief Scientific,Officer of Genaera, is sc..., "Advancing Obesity Therapeutics" to be held at B...
Other Biology Technology:VioQuest Pharmaceuticals to Present at BIO InvestorForum in San Francisco 2VioQuest Pharmaceuticals to Present at BIO InvestorForum in San Francisco 3MAP Pharmaceuticals Announces Pricing of Its Initial Public Offering 2Genaera to Present at Biotech 2007 and BIO InvestorForum 2Genaera to Present at Biotech 2007 and BIO InvestorForum 3
(Date:11/24/2009)...ibed by traditional healers in Brazil to treat a r...o fever and flu. , Now for the first time, resear...ientifically prove the pain relieving properties o...int. , Testing this ancient South American herb o...was able to show that when prepared as a ,tea, th...
(Date:11/24/2009)...derstanding how sounds associated with Navy sonar ... they hear it at all. , The same type of large i...etect flaws in the space shuttle,s behemoth solid ...side the giant head of a whale. The scans are prov...le,s hearing anatomy using a breakthrough method d...
(Date:11/24/2009)...ries from collapsing have been true life-savers. B...eded -- once the arteries are strengthened, they b...ce but to leave them in place. , Prof. Meital Zi...medical Engineering has developed a new patent-pen...re needed, then dissolves. , "Our new composite ...
Breaking Biology News(10 mins):Got a pain? -- Have a cup of Brazilian mint 2Rocket science leads to new whale discovery 2A coating for life 2Senior Quality Lifestyles Corporation Announces Completion of 24164 855 000 in Financing for The Stayton at Museum Way 61232 1Senior Quality Lifestyles Corporation Announces Completion of 24164 855 000 in Financing for The Stayton at Museum Way 61232 2Senior Quality Lifestyles Corporation Announces Completion of 24164 855 000 in Financing for The Stayton at Museum Way 61232 3H1N1 Vaccinations Jam Phone Lines Increased Demand for TimeCenter Online Scheduling Software 61230 1H1N1 Vaccinations Jam Phone Lines Increased Demand for TimeCenter Online Scheduling Software 61230 2The Nations Best Math and Science Students Earn Top High School Honors and Strive for 24100 000 Scholarship 61228 1The Nations Best Math and Science Students Earn Top High School Honors and Strive for 24100 000 Scholarship 61228 2The Nations Best Math and Science Students Earn Top High School Honors and Strive for 24100 000 Scholarship 61228 3
...ews who gripe about poor cabin air quality could h..., hair and clothing. A study in the current issue ... interactions between body oils and ozone found in...emical byproducts that might worsen nasal irritati... air traveler complaints. , In simulated flights ...
...nfects up to 500,000 people in the UK alone, many ...gle biggest cause of people requiring a liver tran...with groups across Europe and the USA, scientists ...lonal antibodies which may be a significant step t...nsive and not successful in all patients. Untreate...
...2007 - The term "biotechnology" elicits a range of...ty. Alan McHughen from the University of Californi...current state public knowledge of biotech, popular...e of the media. How is coexistence dealt with in t...iotech only big companies or also the society at ...
Other Biology News:Skin oil -- ozone interactions worsen air quality in airplanes 2Possible hepatitis C vaccine 2How the public perceives biotech 2
Goat Anti-TNFalpha-IP 2 (G-17) Polyclonal Antibody, Unconjugated from Santa Cruz Biotechnology, Inc.
Goat Anti-Derlin-2 (C-18) Polyclonal Antibody, Unconjugated from Santa Cruz Biotechnology, Inc.
Mouse Anti-Trx-2 Monoclonal Antibody, Unconjugated, Clone 71G4 from Santa Cruz Biotechnology, Inc.
cAMP Competitive immunoassay Assay Kit, HTRF from Cisbio
Biology Products: