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Using the TripleMaster PCR System for Robust Amplification of ,,, GC-Rich DNA Templates

and Roche systems producing slightly less yield. Roche amplified the MC1R target without GC-RICH Resolution Solution and with GC-RICH Resolution Solution concentrations of 0.5 M and 1.0 M. Roche did not amplify any products with concentrations of 1.5 M or 2.0 M (data not shown).

Fig. 2: The TripleMaster PCR System compared to leading suppliers in the amplification of a GC-rich template from the human MC1R gene.
Lanes:


M New England Biolabs 100 bp Marker (0.5 g) 1 Eppendorf, standard thermal cycling protocol 2 Eppendorf, touchdown PCR protocol 3 Invitrogen, touchdown PCR protocol 4 Roche, touchdown PCR protocol, without GC-RICH Resolution Solution 5 Roche, touchdown PCR protocol, with 0.5 M GC-RICH Resolution Solution 6 Roche, touchdown PCR protocol, with 1.0 M GC-RICH Resolution Solution
Discussion

The TripleMaster system required the use of DMSO (dimethyl sulfoxide) to improve the yield and specificity of the PCR reactions for these specific GC-rich templates.
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