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Using the TripleMaster PCR System for Robust Amplification of ,,, GC-Rich DNA Templates

roducts. The Roche system did not amplify any PCR products for all concentrations of the GC-RICH Resolution Solution (data not shown).

Fig. 1: The TripleMaster PCR System compared to leading suppliers in the amplification of GC-rich templates from the human apo E and RB genes.
Lanes:

M New England Biolabs 100 bp Marker (0.5 g) 1 Eppendorf, apo E gene 2 Eppendorf, RB gene 3 Invitrogen, apo E gene 4 Invitrogen, RB gene 5 Roche, apo E gene, without GC-RICH Resolution Solution 6 Roche, apo E gene, with 0.5 M GC-RICH Resolution Solution
Results Experiment 2

The initial thermal cycling conditions used for the MC1R target with the TripleMaster system amplified the desired 760 bp MC1R gene target with excellent yield, but also amplified multiple non-specific products (see Fig. 2). However when the touchdown PCR cycling protocol was used, all three systems amplified only the desired target with outstanding yield. The TripleMaster system amplified the most robust product, with the Invitrogen
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