Navigation Links
Using the TripleMaster PCR System for Robust Amplification of ,,, GC-Rich DNA Templates

roducts. The Roche system did not amplify any PCR products for all concentrations of the GC-RICH Resolution Solution (data not shown).

Fig. 1: The TripleMaster PCR System compared to leading suppliers in the amplification of GC-rich templates from the human apo E and RB genes.
Lanes:

M New England Biolabs 100 bp Marker (0.5 g) 1 Eppendorf, apo E gene 2 Eppendorf, RB gene 3 Invitrogen, apo E gene 4 Invitrogen, RB gene 5 Roche, apo E gene, without GC-RICH Resolution Solution 6 Roche, apo E gene, with 0.5 M GC-RICH Resolution Solution
Results Experiment 2

The initial thermal cycling conditions used for the MC1R target with the TripleMaster system amplified the desired 760 bp MC1R gene target with excellent yield, but also amplified multiple non-specific products (see Fig. 2). However when the touchdown PCR cycling protocol was used, all three systems amplified only the desired target with outstanding yield. The TripleMaster system amplified the most robust product, with the Invitrogen
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11

Related biology technology :

1. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
2. Oil-Free PCR Using the Hot Top Assembly
3. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
4. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
5. Using XL1-Red Mutator Strain to Generate Esterase Variants
6. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
7. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
8. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
9. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
10. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
Post Your Comments:
(Date:7/30/2014)... 30, 2014   Epic Sciences, Inc. ("Epic"), ... diagnostics to personalize and advance the treatment and management ... T. Lucier as chairman of Epic,s board of ... Life Technologies (formerly Invitrogen). Mr. Lucier joins Epic,s board ... circulating rare cell analysis platform with special focus on ...
(Date:7/30/2014)... Connecticut Center for Advanced Technology, Inc. ... Hartford, Conn., was awarded a Rural Business Enterprise Grant ... that will determine the feasibility of implementing an ... Rural Development provided $53,000 for CCAT under the Rural ... of the feasibility study in fiscal year 2014. , ...
(Date:7/30/2014)... LayerBio, Inc., an MIT spinoff developing novel ... of seed financing from friends and family investors. Proceeds ... a novel sustained-release product for ophthalmology. , "We are ... commitment to this important work," said Dr. Ken Mandell, ... many areas in which innovations in drug delivery have ...
(Date:7/29/2014)... (PRWEB) July 29, 2014 The advanced ... the brain that are driving a revolution in the ... new peer-reviewed journal published by SPIE, the international ... issue coincides with the launch of the BRAIN Initiative ... articles that lay out a vision for and provide ...
Breaking Biology Technology:Epic Sciences Appoints Greg Lucier as Chairman of the Board of Directors 2Epic Sciences Appoints Greg Lucier as Chairman of the Board of Directors 3USDA Grant Funds Second Phase of Bio-Energy Research Community Economic Development in CT 2USDA Grant Funds Second Phase of Bio-Energy Research Community Economic Development in CT 3SPIE Launches 'Neurophotonics' Journal with Special Section on BRAIN Initiative 2SPIE Launches 'Neurophotonics' Journal with Special Section on BRAIN Initiative 3SPIE Launches 'Neurophotonics' Journal with Special Section on BRAIN Initiative 4
... Fla., April 9 Cryo-Cell International, Inc. ... world,s largest and most established family cord blood banks, ... 28, 2009. Consolidated revenues for the first quarter ... approximately $4.2 million for the first quarter of fiscal ...
... the release of it,s advanced nutritional supplement, Caplex, ... a unique patent pending formulation created by ... provides cancer patients a comprehensive source of the ... on results accumulated from medical research. Impressive changes ...
... NANTONG, China, April 8 Sinobiopharma Pharmaceutical Group ... the appointment of Ms. Yan Tan as Director ... Co., Ltd., Netherlands. She served as a China-based ... sold to Schering-Plough. Prior to Organon, Ms. Tan ...
Cached Biology Technology:Cryo-Cell International, Inc. Reports First Quarter 2009 Results 2Cryo-Cell International, Inc. Reports First Quarter 2009 Results 3Cryo-Cell International, Inc. Reports First Quarter 2009 Results 4Cancer Fighting Nutrition Supplement, Caplex, Offers Cancer Patients A New All-Natural Treatment Option 2Sinobiopharma, Inc. Appoints New Director of Marketing 2
(Date:7/30/2014)... SAN JOSE, Calif. , July 30, 2014 ... industry-academia partnership with the United States Air Force ... project proposed by Binghamton ... for human performance monitoring (HPM). The $425,000 project, ... and electronics packaging firm i3 Electronics, Inc. ( ...
(Date:7/30/2014)... the journal Scientific Reports , published by ... found that while global temperature is indeed increasing, so ... while each year,s average hottest and coldest temperatures will ... within a wider range of potential high and low ... that even as overall temperatures rise, we may still ...
(Date:7/29/2014)... advanced optical methods and applications for imaging and manipulation ... the neurosciences were the inspiration behind Neurophotonics , ... society for optics and photonics. , The first issue ... the United States, and a special section features articles ... of the impact that optics and photonics will have ...
Breaking Biology News(10 mins):Nano-Bio Manufacturing Consortium Awards Funding to Binghamton University for Development of Electronics and Biometric Sensor Platforms 2Nano-Bio Manufacturing Consortium Awards Funding to Binghamton University for Development of Electronics and Biometric Sensor Platforms 3Big data confirms climate extremes are here to stay 2SPIE launches Neurophotonics journal with special section on BRAIN Initiative 2SPIE launches Neurophotonics journal with special section on BRAIN Initiative 3
... Alberta researcher has discovered a rare practice by red squirrels ... its heart is also a survival tactic. U ... when he discovered a female had adopted a newborn squirrel ... candidate in evolutionary biology, found a baby red squirrel had ...
... funded by the U.S. economic stimulus program (ARRA) Health ... to advance the use of health information technology (HIT) ... been awarded to leading members of the American Medical ... the last two decades has advocated for informatics as ...
... and Space Sciences More than 2,200 scientists are ... sciences in Foz do Iguau at the 2010 Meeting of ... 8 to 12 August, are planning 377 scientific sessions on ... weather events in the Americas, climate impacts of megacities, greenhouse ...
Cached Biology News:Red squirrels: Altruists or self-serving survivalists? 2AMIA Members Manage HITECH Stimulus Grant Awards 22010 Meeting of the Americas, Brazil: News media registration open 22010 Meeting of the Americas, Brazil: News media registration open 32010 Meeting of the Americas, Brazil: News media registration open 4
Anti-GOSR2 Monoclonal AntibodyOther Sub-Family: not assigned-Other Applications: Immunocytochemistry, Immunoprecipitation, Western Blot...
Mouse monoclonal [4A2.2] to SLC6A4 ( Abpromise for all tested applications). entrezGeneID: 6532 SwissProtID: P31645...
... combines shaking and rocking for maximal fluid movement. ... mm stainless steel or acrylic platform, it is ... operation. The platform angle is easily adjusted from ... action and rotation speed of platform is adjusted ...
Anti-DNA Polymerase a, Human Description: 50 l Research Focus: cancer & cell proliferation Storage: 4C Shipping Temperature: 4C...
Biology Products: