( 20 sec. ...)Using the TripleMaster PCR System for Robust Amplification of ,,, GC-Rich DNA Templates

20 sec. 35x 69C 10 sec. 35x 72C 30 sec. Human apo E gene (268 bp) 1x 95C 5 min. 35x 95C 20 sec. 35x 60C 10 sec. 35x 72C 30 sec. Human MC1R gene (760 bp) 1x 95C 5 min. 25x 94C 1 min. 25x 63C with 0.5C/cycle decrease 1 min. 25x 72C 1 min. 30 sec. 10x 94C 1 min. 10x 50C 1 min. 10x 72C 1 min. 1x 72C 10 min. Results Experiment 1

All three PCR systems amplified the 268 bp apo E gene target, with TripleMaster producing the highest yield (see Fig. 1). The Invitrogen and Roche systems produced approximately equal yields, with Roche amplifying the desired product without GC-RICH Resolution Solution and with 0.5 M GC-RICH Resolution Solution. Roche did not amplify any products with GC-RICH Resolution Solution concentrations of 1.0 M, 1.5 M, or 2.0 M (data not shown).

The TripleMaster and Invitrogen systems amplified the 180 bp RB gene target with approximately equal yield (see Fig. 1). However, the Invitrogen system also amplified non-specific p
'"/>

Source:

Page: All 1 2 3 4 5 6 7 8 9 10 11
Related biology technology :

1. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
2. Oil-Free PCR Using the Hot Top Assembly
3. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
4. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
5. Using XL1-Red Mutator Strain to Generate Esterase Variants
6. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
7. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
8. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
9. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
10. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy