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Using the TripleMaster PCR System for Robust Amplification of ,,, GC-Rich DNA Templates

20 sec. 35x 69C 10 sec. 35x 72C 30 sec. Human apo E gene (268 bp) 1x 95C 5 min. 35x 95C 20 sec. 35x 60C 10 sec. 35x 72C 30 sec. Human MC1R gene (760 bp) 1x 95C 5 min. 25x 94C 1 min. 25x 63C with 0.5C/cycle decrease 1 min. 25x 72C 1 min. 30 sec. 10x 94C 1 min. 10x 50C 1 min. 10x 72C 1 min. 1x 72C 10 min. Results Experiment 1

All three PCR systems amplified the 268 bp apo E gene target, with TripleMaster producing the highest yield (see Fig. 1). The Invitrogen and Roche systems produced approximately equal yields, with Roche amplifying the desired product without GC-RICH Resolution Solution and with 0.5 M GC-RICH Resolution Solution. Roche did not amplify any products with GC-RICH Resolution Solution concentrations of 1.0 M, 1.5 M, or 2.0 M (data not shown).

The TripleMaster and Invitrogen systems amplified the 180 bp RB gene target with approximately equal yield (see Fig. 1). However, the Invitrogen system also amplified non-specific p
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