( 1x; 1.5 mM 1x; 1.5 mM ...)Using the TripleMaster PCR System for Robust Amplification of ,,, GC-Rich DNA Templates

1x; 1.5 mM 1x; 1.5 mM 1x; 1.5 mM dNTP's 200 M each 200 M each 200 M each Primers 0.4 M each 0.4 M each 0.4 M each ThermalAce DNA Polymerase 2.0 U 2.0 U 2.0 U Template DNA 100 ng 100 ng 100 ng Roche GC-RICH PCR System Human RB gene(180 bp) Human apo E gene (268 bp) Human MC1R gene (760 bp) GC-RICH PCR Buffer with Mg²⁺ and DMSO 1x; 1.5 mM; 5% 1x; 1.5 mM; 5% 1x; 1.5 mM; 5% GC-RICH Resolution Solution 0 2 M 0 2 M 0 2 M dNTP's 200 M each 200 M each 200 M each Primers 0.4 M each 0.4 M each 0.4 M each GC-RICH PCR Enzyme mix 2.0 U 2.0 U 2.0 U Template DNA 100 ng 100 ng 100 ng Table 3: Thermal cycling parameters for GC-rich PCR targets. Target Cycle Temperature Time Human RB gene (180 bp) 1x 95C 5 min. 35x 95C
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