Navigation Links
Using the TripleMaster PCR System for Robust Amplification of ,,, GC-Rich DNA Templates

1x; 1.5 mM 1x; 1.5 mM 1x; 1.5 mM dNTP's 200 M each 200 M each 200 M each Primers 0.4 M each 0.4 M each 0.4 M each ThermalAce DNA Polymerase 2.0 U 2.0 U 2.0 U Template DNA 100 ng 100 ng 100 ng Roche GC-RICH PCR System Human RB gene(180 bp) Human apo E gene (268 bp) Human MC1R gene (760 bp) GC-RICH PCR Buffer with Mg2+ and DMSO 1x; 1.5 mM; 5% 1x; 1.5 mM; 5% 1x; 1.5 mM; 5% GC-RICH Resolution Solution 0 2 M 0 2 M 0 2 M dNTP's 200 M each 200 M each 200 M each Primers 0.4 M each 0.4 M each 0.4 M each GC-RICH PCR Enzyme mix 2.0 U 2.0 U 2.0 U Template DNA 100 ng 100 ng 100 ng Table 3: Thermal cycling parameters for GC-rich PCR targets. Target Cycle Temperature Time Human RB gene (180 bp) 1x 95C 5 min. 35x 95C
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11

Related biology technology :

1. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
2. Oil-Free PCR Using the Hot Top Assembly
3. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
4. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
5. Using XL1-Red Mutator Strain to Generate Esterase Variants
6. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
7. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
8. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
9. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
10. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
Post Your Comments:
(Date:7/26/2014)... Canada Endoscopy Visualization Systems Market Outlook ... Canada Endoscopy Visualization Systems market. The report provides ... units) and average prices (in US dollars) within ... Endoscopy Visualization Systems and Low-End Endoscopy Visualization Systems. ... , The report also provides company shares and ...
(Date:7/25/2014)... July 26, 2014 "Ischemic Stroke ... 2014" provides data on the Ischemic Stroke and ... elemental information and data relating to the clinical ... View full global Ischemic Stroke Research Report 2014 ... overview of the trial numbers and their recruitment ...
(Date:7/25/2014)... Calif. , July 25, 2014 Amgen ... of Directors today declared a $0.61 per share dividend ... be paid on Sept. 5, 2014, to all stockholders ... Aug. 14, 2014.  About Amgen Amgen ... patients suffering from serious illnesses by discovering, developing, manufacturing ...
(Date:7/25/2014)... each person,s digestive tract. Scientists believe that some of ... infections, but their role in human health is not ... role of these bacteria, a team of researchers led ... in the bacterial populations of two research subjects over ... 25 issue of the journal Genome Biology , ...
Breaking Biology Technology:Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 2Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 3Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 4Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 5Ischemic Stroke and Cerebral Stroke Global Clinical Trials Review H2, 2014: MarketResearchReports.Biz 2Ischemic Stroke and Cerebral Stroke Global Clinical Trials Review H2, 2014: MarketResearchReports.Biz 3Amgen Announces 2014 Third Quarter Dividend 2Amgen Announces 2014 Third Quarter Dividend 3Monitoring the rise and fall of the microbiome 2Monitoring the rise and fall of the microbiome 3
... April 4, 2011 Reportlinker.com announces that ... in its catalogue: Antiviral ... http://www.reportlinker.com/p0203535/Antiviral-Therapeutics---Technologies-markets-and-companies.html ... of antiviral approaches including vaccines, pharmaceuticals and ...
... 2011 Reportlinker.com announces that a new market ... Nanobiotechnologies- applications, markets and companies ... Nanotechnology is the creation and utilization of materials, ... on the nanometer-length scale (a nanometer is one ...
... 4, 2011 GeneLink, Inc. (OTCBB: GNLK, "the ... reported financial results for the fiscal year ended December 31, ... Gross margin increased from $4,906,710 in 2009 to $4,997,367 in ... 2010 as compared to $8,561,455 in 2009; Operating losses ...
Cached Biology Technology:Reportlinker Adds Antiviral Therapeutics - Technologies, Markets and Companies 2Reportlinker Adds Antiviral Therapeutics - Technologies, Markets and Companies 3Reportlinker Adds Antiviral Therapeutics - Technologies, Markets and Companies 4Reportlinker Adds Nanobiotechnologies - Applications, Markets and Companies 2Reportlinker Adds Nanobiotechnologies - Applications, Markets and Companies 3GeneLink Reports 2010 Results 2
(Date:7/25/2014)... SYRACUSE, N.Y., July 25, 2014 In the first broad-scale ... Forest Service scientists and collaborators calculated that trees are ... preventing 670,000 incidences of acute respiratory symptoms. , While ... improvement of less than 1 percent, the impacts of ... effects of the reduced air pollution at nearly $7 ...
(Date:7/25/2014)... 100,000s of people lived on the land now called ... control the availability of plants they used for food, ... fire to maintain desired habitat and natural resources. , ... Pacific Southwest Station, will lead a field trip ... the Ecological Society of America,s 99th Annual Meeting, in ...
(Date:7/25/2014)... from the Terra satellite show the different areas that have ... Russia. The burn scars show up as reddish-brown splotches ... broken across the remote parts of Eastern Russia in the ... MODIS instrument, it is still possible to see the ... features noted below show the devastating number of fires that ...
Breaking Biology News(10 mins):First national study finds trees saving lives, reducing respiratory problems 2Fire ecology manipulation by California native cultures 2Fire ecology manipulation by California native cultures 3
... around the ancient spruce woodlands of eastern North ... could respond to climate change? Matt Fitzpatrick of ... Appalachian Laboratory, along with researchers from the University ... received a three-year, $670,000 award from the National ...
... Uppsala University, Sweden, instil hope of efficient hydrogen production with ... scepticism based on previous research. The study, which is published ... view on the ability of green algae which is ... producing fuel from renewable energy sources to replace the fossil ...
... Monell Center molecular neurobiologist Peihua Jiang, PhD, is ... Investigators in Gustation, an annual award presented by the ... scientist who is a rising leader in the field ... the understanding of the structure and function of taste ...
Cached Biology News:Scientists look to ancient past to better predict how species may respond climate change 2Surprising findings on hydrogen production in green algae 2Peihua Jiang receives Ajinomoto Award for Young Investigators in Gustation from AChemS 2