Navigation Links
Using the TripleMaster PCR System for Robust Amplification of ,,, GC-Rich DNA Templates

Eppendorf TripleMaster PCR System
Invitrogen ThermalAce DNA Polymerase Kit
Roche GC-RICH PCR System
Genomic DNA isolated using the Eppendorf Perfect gDNA Blood Mini Kit
Eppendorf Mastercycler Gradient Thermal Cycler
EasyCast Horizontal Minigel System (Owl Separation Systems)
SeaKem LE Agarose (BioWhittaker Molecular Applications)
1x TBE (Eppendorf)
Ethidium Bromide (Sigma)
Alpha Imager CCD Camera Documentation System (Alpha Innotech Corporation)


Methods

The performance of the TripleMaster PCR System for GC-rich PCR was tested against other PCR systems specifically designed for GC-rich PCR. Three different DNA sequences with high GC-content were chosen for amplification. The primer sequences, length of the expected PCR products and percentage of GC content in the amplified sequences are listed in Table 1. Genomic DNA was isolated from whole blood preserved in EDTA using the Eppendorf Perfect gDNA Blood Mini Kit. The oligonucleotide primers were obtained from Sigma-Genosys. Triplicate reactions were prepared in accordance with the manufacturers recommended specifications and all PCR reagents were used at final concentrations within the ranges recommended by the manufacturer (see Table 2). The 1.6x Eppendorf Tuning Buffer concentration (with 4.0 mM Mg2+) was used for the apolipoprotein E and retinoblastoma gene reactions due to the size of the targets. The TripleMaster PCR System user manual recommends a higher buffer concentration for targets less than 500 bp because the Tuning Buffer is a low-salt buffer specifically designed for long-range PCR, and short targets require a buffer with a higher salt con
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11

Related biology technology :

1. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
2. Oil-Free PCR Using the Hot Top Assembly
3. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
4. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
5. Using XL1-Red Mutator Strain to Generate Esterase Variants
6. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
7. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
8. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
9. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
10. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
Post Your Comments:
(Date:7/29/2014)... Rock Hill, South Carolina (PRWEB) July 29, 2014 ... communicate with teams of people where cell phone masts or ... other iPhones up to 100 feet away without the need ... iPhones to target longer ranges. , The new app has ... 8% of US homes are at very high risk of ...
(Date:7/29/2014)... TX (PRWEB) July 29, 2014 G-CON ... autonomous modular cleanrooms, today announced a new leadership appointment ... the United States and abroad. , Maik Jornitz, ... as the company’s President charged with leading the pioneering ... “As we enter this stage of development and look ...
(Date:7/29/2014)... (PRWEB) July 29, 2014 The prestigious ... of Congress was recently awarded to Juliana Hillis and ... MA, for their project, “From an Egg, Everything: America’s ... 3,000 final entries for the National History Day program ... 300 historians and educators in Washington, D.C. , The ...
(Date:7/29/2014)... EXpressLO LLC, expert provider of focused ion ... has been granted patent number 8,789,826 by the U.S. ... a unique specimen carrier support grid design described in ... patent for EXpressLO LLC awarded to founder and President, ... patents extend EXpressLO’s portfolio of intellectual property. The EXpressLO™ ...
Breaking Biology Technology:DisasterTalk App Could Save Lives as Report Reveals 8% of US Homes at Very High Risk of Natural Disaster 2G-CON Manufacturing, Inc. Announces Maik Jornitz as President 2In Vitro Fertilization (IVF) was the Subject of This Year’s Winning Entry for the Prestigious Library of Congress “Discovery or Exploration in History Award” 2In Vitro Fertilization (IVF) was the Subject of This Year’s Winning Entry for the Prestigious Library of Congress “Discovery or Exploration in History Award” 3EXpressLO LLC Granted Second U.S. Patent for Innovative Specimen Preparation Methods 2
... diodes, require a transparent conductive layer with a high ... into an organic layer to produce more light. ... Conference on Science and Technology of Synthetic Metals in ... efficiency. A balanced injection of positive and negative charge ...
... Partnership to provide expertise and applications support in plant ... ... LOUIS, July 8 Sigma-Aldrich (Nasdaq:,SIAL) and Metahelix Life Sciences ... applications data for Sigma-Aldrich,s,plant bioscience product line, and provide expert ...
... 8 Biotech and pharmaceutical,firms are developing ... streamline the,complicated drug discovery process, reports Genetic ... rely on a combination,of high-throughput screening methods, ... an article in the July issue,( http://www.genengnews.com/articles/chitem.aspx?aid=2527 ...
Cached Biology Technology:Research helps understand factors that influence efficiency of organic-based devices 2Research helps understand factors that influence efficiency of organic-based devices 3Sigma-Aldrich Announces Collaboration With Metahelix Life Sciences 2Sigma-Aldrich Announces Collaboration With Metahelix Life Sciences 3GEN Reports on Novel Hit-to-Lead Drug Discovery Strategies 2
(Date:7/29/2014)... of a stroke is an immensely complex challenge ... as effective therapy. Advancing such knowledge to improve ... focus of research by Sergei Adamovich, associate professor ... faculty more than a decade ago. It is ... from sources such as the National Institutes of ...
(Date:7/29/2014)... method to create DNAprotein conjugates. The method can ... , DNA linked to proteins including antibodies ... used in diagnostic techniques, nanotechnology and other disciplines. ... can be used for purposes such as ... The method also provides easier access to handling ...
(Date:7/29/2014)... 2014 With thirty days to go and almost ... 2014 Conference are well underway. The event, a joint ... EMBO, and the French Society for Biochemistry and Molecular ... to Thursday, 4 September at the Palais des Congrs ... the largest meeting for the life sciences in Europe ...
Breaking Biology News(10 mins):Continuing the quest for better stroke therapies 2Continuing the quest for better stroke therapies 3New method provides researchers with efficient tool for tagging proteins 2Counting down to FEBS-EMBO 2014 in Paris, France 2
... Naturforschung will collaborate on the publication of two ... Palaeobiodiversity and Palaeoenvironments and Marine Biodiversity ... through Springer,s abstracting and indexing services and innovative ... both electronic and print versions. Palaeobiodiversity ...
... Current research suggests that a new technique to ... tissue samples. The related report by Balic et ... tissues from colorectal cancer patients using quantitative high-resolution melting ... Journal of Molecular Diagnostics . DNA in ...
... genome eight years ago, enormous progress has been made ... most human genes is still barely understood. An important ... or protein is to compare its sequence with the ... easier to investigate. From the functions of related genes ...
Cached Biology News:Springer and Senckenberg society to collaborate on life sciences and geosciences journals 2New technique for cancer screening 2Relationships in rank and file 2Relationships in rank and file 3Relationships in rank and file 4
... the standard for sample concentration and drying. ... uses a combination of centrifugal force, heat and ... run. , SC210A comes equipped with:, ... chemical compatibility ...
... RC Maxi MB (for aqueous, other low ... evaporator systems respond to specific applications of ... safe concentration of heat-labile samples. The RC ... of cold traps and a wide selection ...
... The Jouan RC Standard Bio for ... applications of molecular biology and biochemistry for ... The RC systems can be expanded with ... wide selection of rotors (eliminating sample loss ...
... The Jouan RC10 series of vacuum concentrators/centrifugal ... protection features allied to rapid and safe ... can be combined with oil-free or high ... and a wide selection of rotors (eliminating ...
Biology Products: