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Using the TripleMaster PCR System for Robust Amplification of ,,, GC-Rich DNA Templates

eksler, editors), Cold Spring Harbor Laboratory Press, NY, pp. 5362.
  • Schuchard, M., G. Sarkar, T. Ruesink and T.C. Spelsberg (1993) Two-step hot PCR amplification of GC-rich avian c-myc sequences. Biotechniques. 14: 390394.
  • Sun, Y., G. Hegamyer and N.H. Colburn (1993) PCR-direct sequencing of a GC-rich region by inclusion of 10% DMSO: application to mouse c-jun. Biotechniques. 15: 372374.
  • TripleMaster PCR System user manual (2000) Version TM1000, Eppendorf.
  • Turner, S.L. and F.J. Jenkins (1995) Use of deoxyinosine in PCR to improve amplification of GC-rich DNA. Biotechniques. 19: 4852.
  • * The Polymerase Chain Reaction (PCR) is covered by U.S. patent numbers 4683105 and 4683202 held by Roche Molecular Systems and requires a license for use.


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