Navigation Links
Using the TripleMaster PCR System for Robust Amplification of ,,, GC-Rich DNA Templates

with the greatest complement to the primers. Although the annealing temperature eventually drops to a level where non-specific products are normally formed, the target amplicon has already commenced its exponential amplification and therefore surpases any non-specific products during these cycles. Touchdown PCR is a valuable method for generating robust PCR reactions without time-consuming optimization experiments.2

In conclusion, the TripleMaster Enzyme Mix combines the efficiency of Taq DNA Polymerase with the 3' 5' exonuclease activity of a proofreading thermostable polymerase, along with a polymerase-enhancing factor that provides an extremely high extension rate and maximal proofreading assisted fidelity. The TripleMaster Tuning Buffer, specifically designed for long-range PCR, can also be used for the amplification of short, GC-rich targets. In addition, the TripleMaster High Fidelity Buffer can be used for high-fidelity PCR as well as the amplification of short, GC-rich targets (data not shown). All of these innovative components make the TripleMaster PCR System a versatile kit that can be used for the amplification of GC-rich targets, long-range PCR, and high-fidelity PCR. One can see how the TripleMaster PCR System is a universal solution for a variety of PCR applications.5

References:

  1. Liu, Q. and S.S. Sommer (1998) Subcycling-PCR for multiplex long-distance amplification of regions with high and low GC content: application to the inversion hotspot in the factor VIII gene. Biotechniques. 25: 10221028.
  2. Roux, K.H. (1995) Optimization and Troubleshooting in PCR. In: PCR Primer, A Laboratory Manual (Dieffenback, C.W. and G.S. Dv
    '"/>


Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11

Related biology technology :

1. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
2. Oil-Free PCR Using the Hot Top Assembly
3. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
4. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
5. Using XL1-Red Mutator Strain to Generate Esterase Variants
6. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
7. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
8. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
9. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
10. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
Post Your Comments:
(Date:1/22/2015)... (PRWEB) January 22, 2015 Shimadzu ... new UV-1280 multipurpose UV-visible spectrophotometer. Offering wavelength scanning ... instrument is ideal for applications in a variety ... science. , The user-friendly UV-1280 enables intuitive operation, ...
(Date:1/22/2015)... Cambridge Semantics, the leading provider of smart ... 2014 was a record-breaking year across the board for the ... Smart Data Platform and our Smart Data solutions, our customers ... data which led to record growth for the company in ...
(Date:1/22/2015)... California (PRWEB) January 22, 2015 Pipette.com ... Offer to their comprehensive portfolio of Eppendorf products. ... customers to purchase a Centrifuge 5424/5424 R and receive ... of either Eppendorf Research plus or Eppendorf Reference 2 ...
(Date:1/22/2015)... Geneva, Switzerland (PRWEB) January 22, 2015 ... for characterized mammalian Research Cell Banks (RCBs) used for ... generated Research Cell Banks will include Next-Generation Sequencing ... cell’s genetic architecture de-risks biologic manufacturing by ensuring the ...
Breaking Biology Technology:Shimadzu’s New Monitored Single-Beam UV-Vis Spectrophotometer Offers Comprehensive Measurement Options in a Compact Body 2Cambridge Semantics Announces Record Results for 2014 2Eppendorf Announces Promotional Bundle Which Includes: Eppendorf Centrifuge 5424, 3-Pack of Pipettes, and Tips - Available Now at Pipette.com 2Selexis Generated Research Cell Banks Now Fully Sequenced Using Next-Generation Sequencing 2
... Lisa Marsh Carsten Carstens ,Stratagene , ... expression ,vectors offers unparalleled convenience. The drug-resistance ... without subcloning. Through site-specific ,recombination, hygromycin, puromycin, ... efficiently inserted into the pExchange core vectors. ...
... LipoTAXI transfection reagent: A proven ... ,Stratagene , LipoTAXI ... cells and cells growing in,suspension. In this article, ... the effectiveness of LipoTAXI reagent. Optimizing transfection,conditions is ...
... of 96 PCR products , , Scott ... , The StrataPrep 96 PCR purification ... This kit offers a high-throughput format,for purifying ultra-pure PCR ... PCR purification kit uses the same silica matrix-binding,technology as ...
Cached Biology Technology:Mammalian Expression Vectors with Interchangeable Selectable Markers 2Mammalian Expression Vectors with Interchangeable Selectable Markers 3Mammalian Expression Vectors with Interchangeable Selectable Markers 4Versatile Transfection Reagent Offers Low Toxicity and Consistent,Performance 2Versatile Transfection Reagent Offers Low Toxicity and Consistent,Performance 3Versatile Transfection Reagent Offers Low Toxicity and Consistent,Performance 4Versatile Transfection Reagent Offers Low Toxicity and Consistent,Performance 5Versatile Transfection Reagent Offers Low Toxicity and Consistent,Performance 6Versatile Transfection Reagent Offers Low Toxicity and Consistent,Performance 7High-Throughput System Generates Ultra-Pure PCR Products Suitable for All,Applications 2High-Throughput System Generates Ultra-Pure PCR Products Suitable for All,Applications 3High-Throughput System Generates Ultra-Pure PCR Products Suitable for All,Applications 4High-Throughput System Generates Ultra-Pure PCR Products Suitable for All,Applications 5
(Date:1/22/2015)... Technology Showcase, Hall E -   EyeLock Inc. , ... it will showcase its EyeLock ID technology integrated in a ... Energy,s Oak Ridge National Laboratory (ORNL) at the ... identity authentication technology is being used to validate the driver ...
(Date:1/22/2015)... Washington , January 20, 2015 Investorideas.com, ... tech stocks, releases video from the CES 2015 (Consumer Electronics ... Apollo Robbins . Apollo shows how easily ... smart wallet, a product of NXT-ID, Inc. (NASDAQ: ...
(Date:1/22/2015)... EyeLock, Inc. , a market leader of iris-based identity authentication ... to the new role of Senior Vice President of Mobile ... mobile platforms and wearable solutions for EyeLock,s technologies. Gerber brings ... semiconductor industry to his role at EyeLock, with expertise in ...
Breaking Biology News(10 mins):EyeLock Debuts First Iris Authentication Technology Embedded in 3D Printed Automobile 2EyeLock Debuts First Iris Authentication Technology Embedded in 3D Printed Automobile 3Apollo Robbins Talks about the Illusion of Personal Safety and How Easily Your Wallet is Stolen; If a Wocket Wallet is Stolen the Thief Can't do Anything With it! 2Apollo Robbins Talks about the Illusion of Personal Safety and How Easily Your Wallet is Stolen; If a Wocket Wallet is Stolen the Thief Can't do Anything With it! 3EyeLock Names Steve Gerber as Senior Vice President of Mobile and Wearables 2
... researchers have demonstrated a close correlation between the decline ... system and the progression of chronic obstructive pulmonary disease ... decreases the flow of air through the lungs as ... lung tissue samples from COPD patients by scientists at ...
... Media,Group,s, C4ISR Journal will host its 8th Annual C4ISR ... October 16-17, 2008 at the Sheraton,Crystal City Hotel, Arlington, ... C4ISR technology through,various conference sessions including:, ... Sharing for Homeland Defense/Civil Support -- From Net-Centric ...
... MANHATTAN, KAN. -- New findings about the causes of ... genetic. "We now know it,s also in the ... Professor of Diagnostic Medicine and Pathobiology at Kansas State ... ago, Richt said, it was thought that the cattle ...
Cached Biology News:Key component of debilitating lung disease identified 2Annual C4ISR Integrated Conference and Big 25 Awards Banquet 2Annual C4ISR Integrated Conference and Big 25 Awards Banquet 3K-State professor's USDA research shows mad cow disease also caused by genetic mutation 2
S. O'Hare & C.K. Atterwill • In vitro toxicity techniques for regular lab use. Ex vivo techniques are also covered with the aim of reducing and refining the use of animals in the future....
Form: Concentrated Applications: ELISA...
RABBIT ANTI HALOPERIDOL...
Form: Ready to use Applications: Western Blot, ELISA...
Biology Products: