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Using the TripleMaster PCR System for Robust Amplification of ,,, GC-Rich DNA Templates

with the greatest complement to the primers. Although the annealing temperature eventually drops to a level where non-specific products are normally formed, the target amplicon has already commenced its exponential amplification and therefore surpases any non-specific products during these cycles. Touchdown PCR is a valuable method for generating robust PCR reactions without time-consuming optimization experiments.2

In conclusion, the TripleMaster Enzyme Mix combines the efficiency of Taq DNA Polymerase with the 3' 5' exonuclease activity of a proofreading thermostable polymerase, along with a polymerase-enhancing factor that provides an extremely high extension rate and maximal proofreading assisted fidelity. The TripleMaster Tuning Buffer, specifically designed for long-range PCR, can also be used for the amplification of short, GC-rich targets. In addition, the TripleMaster High Fidelity Buffer can be used for high-fidelity PCR as well as the amplification of short, GC-rich targets (data not shown). All of these innovative components make the TripleMaster PCR System a versatile kit that can be used for the amplification of GC-rich targets, long-range PCR, and high-fidelity PCR. One can see how the TripleMaster PCR System is a universal solution for a variety of PCR applications.5

References:

  1. Liu, Q. and S.S. Sommer (1998) Subcycling-PCR for multiplex long-distance amplification of regions with high and low GC content: application to the inversion hotspot in the factor VIII gene. Biotechniques. 25: 10221028.
  2. Roux, K.H. (1995) Optimization and Troubleshooting in PCR. In: PCR Primer, A Laboratory Manual (Dieffenback, C.W. and G.S. Dv
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