Navigation Links
Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27)


INTRODUCTION
Calibration of a dissolution apparatus is necessary to produce accurate results for dissolution testing of pharmaceutical dosage forms and to allow comparison between batches and between laboratories. USP requirements for calibration are given in the Apparatus Suitability Test in USP <711> Dissolution.1 Each individual apparatus (i.e. spindle or basket) must be tested with disintegrating type and non-disintegrating type USP dissolution calibrators. The calibrator tablets available from the USP-NF include prednisone, (50 mg, disintegrating type) and salicylic acid (300 mg, non-disintegrating type). The test involves determining the percentage of drug that dissolves in 900 mL dissolution medium under specified conditions (50 or 100 RPM) after a specified time interval (30 minutes). The apparatus is deemed suitable if the results are within the acceptable range stated in the certificate for the calibrator (i.e the specifications sheet for that particular lot number of calibrator).

USP <711> specifies that both prednisone and salicylic acid are to be measured by UV spectrophotometry (242 nm and 296 nm) by comparison to a standard solution of known concentration. (Compendial requirements for UV spectrophotometry are given in USP <851> Spectrophotometry and Light Scattering.2)

This application note describes how spectrophotometric analyses for dissolution calibration can be quickly and conveniently carried out in a 96-well format using the SPECTRAmax PLUS microplate spectrophotometer. The PathCheck feature of the SPECTRAmax PLUS automatically corrects the results to a 1 cm pathlength, therefore samples read in a microplate give the same values that they would if read in a standard cuvette. Accurate pipetting of the samples into the wells is not necessary; indeed PathCheck corrects for pipetting errors. Using flexible custom formulas and the spreadsheet capability of SOFTmax PR
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
2. Oil-Free PCR Using the Hot Top Assembly
3. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
4. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
5. Using XL1-Red Mutator Strain to Generate Esterase Variants
6. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
7. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
8. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
9. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
10. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy

Post Your Comments:
(Date:12/1/2009)...oday announced a collaborative research agreement ...valuating EpiVax’s proprietary Tregitope tec...l immune responses to certain drugs. In the course...gitope technology will be evaluated for its effect... , (Vocus) December 2, 200...
(Date:12/1/2009)...alifornia and AMSTERDAM, December 1 /PRNewswire/ -...nostics, announced today that,Palmetto GBA, Califo...hed,coding guidelines for the company,s MammaPrint...cancer recurrence test, which has been reimbursed ...he United States can now submit claims for the,Mam...
(Date:12/1/2009)... /PRNewswire-FirstCall/ - BioSpec Global Solutions...nced today that the company has contracted with RM...he manufacturing of the TOG,S 3000 and 9000(TM) eq...e seen the disastrous results of food contaminatio...s in the past" said Don Saunders President and CEO...
(Date:12/1/2009)...tarian organizations will collaborate in comprehen...r technologies. Their shared efforts will begin in... , Dallas, TX (PRWEB) Dec...randum of Understanding, Mercy Ships and MediSend ...p develop sustainable infrastructure in hospitals ...
Breaking Biology Technology:EpiVax, Inc. forms Collaborative Research Agreement with Roche to Develop Tregitope Technology 2EpiVax, Inc. forms Collaborative Research Agreement with Roche to Develop Tregitope Technology 3Medicare Administrator Establishes Reimbursement Coding Policy for Agendia's Breast Cancer Recurrence Test MammaPrint(R) 2Mercy Ships and MediSend International Partner to Deliver Hope and Healing 2Mercy Ships and MediSend International Partner to Deliver Hope and Healing 3
...SP core from CEVA provides unmatched sound quality...ochlear implant system, SAN JOSE, Calif., Sept. 1...g licensor of innovative intellectual,property (IP...onsumer and,multimedia applications, today announc... the CEVA-TeakLite(TM) DSP core in its Harmony HiR...
... PAREXEL Consulting, a,business unit of PAREXEL In...life sciences consultancy serving the bio/pharmace...day that it is strengthening its Good Manufacturin...s global client base,address complexities of Europ...turing regulations, quality, and safety. This ini...
...tember 18 - Clinical Study Demonstrates Breakthr...pile Capacities and Expands Potential Production,C...tion Sanofi pasteur, the vaccines division of san...at its new investigational H5N1 pandemic influenza...d a high immune response,at the lowest dose of H5N...
Other Biology Technology:Advanced Bionics Deploys CEVA-TeakLite(TM) DSP Core to Enhance Its Harmony(TM) HiResolution(R) Bionic Ear System 2Advanced Bionics Deploys CEVA-TeakLite(TM) DSP Core to Enhance Its Harmony(TM) HiResolution(R) Bionic Ear System 3Parexel Consulting Strengthens Good Manufacturing Practice Capabilities in Europe 2Parexel Consulting Strengthens Good Manufacturing Practice Capabilities in Europe 3Parexel Consulting Strengthens Good Manufacturing Practice Capabilities in Europe 4Parexel Consulting Strengthens Good Manufacturing Practice Capabilities in Europe 5Parexel Consulting Strengthens Good Manufacturing Practice Capabilities in Europe 6Sanofi Pasteur's Investigational H5N1 Influenza Vaccine Achieves High Immune Response at low Dosage 2Sanofi Pasteur's Investigational H5N1 Influenza Vaccine Achieves High Immune Response at low Dosage 3Sanofi Pasteur's Investigational H5N1 Influenza Vaccine Achieves High Immune Response at low Dosage 4Sanofi Pasteur's Investigational H5N1 Influenza Vaccine Achieves High Immune Response at low Dosage 5
(Date:11/30/2009)...ors of The Journal of Experimental Biology are p...re National de la Recherche Scientifique (CNRS) in...ner of this year,s JEB Outstanding Paper Prize. ...ding achievement and is intended to encourage youn...or-in-Chief, and adds that ,we truly appreciate wh...
(Date:11/30/2009)...alled invasion proteins are crucial for infection....human cells, which stimulates the host cell to tak...les exert a different function, for example the re...up,s results have now been published in the curren..., Spoiled meat is one of the sources for Listeri...
(Date:11/30/2009)...antage of lower early bird registration rates and ...congress of 2010 at http://www.iofwco-ecceo10.org...of the International Osteoporosis Foundation (IOF)...c Aspects of Osteoporosis and Osteoarthritis (ESCE...orld are expected to attend. The exciting scientif...
Breaking Biology News(10 mins):French scientist wins the Journal of Experimental Biology Outstanding Paper Prize 2009 2French scientist wins the Journal of Experimental Biology Outstanding Paper Prize 2009 3French scientist wins the Journal of Experimental Biology Outstanding Paper Prize 2009 4It takes 2 to infect 2PLC Medical Systems Reports Strong Success for RenalGuard 28R 29 at ESC 2009 56929 1PLC Medical Systems Reports Strong Success for RenalGuard 28R 29 at ESC 2009 56929 2PLC Medical Systems Reports Strong Success for RenalGuard 28R 29 at ESC 2009 56929 3Private Care Association to Tackle Major Issues Impacting Home Care Industry 56925 1Private Care Association to Tackle Major Issues Impacting Home Care Industry 56925 2Ovarian Cancer Coalition Partners With Consuelo Vanderbilt Costin 56921 1Ovarian Cancer Coalition Partners With Consuelo Vanderbilt Costin 56921 2
...ing" may extend more widely into Africa, North Ame...of Oregon geologist,s analysis of an ancient green...ill be warm, but this new information confirms tha...y season will increase dramatically," says Greg Re...eenhouse is brewing is published in the April issu...
...send for many of the two million Americans with bi...ople on the drug may develop hypothyroidism, tremo... urination and gain weight. , However, better trea...erstanding of the still-mysterious mechanism by wh...er. Now, researchers led by Philip Brandish of Mer...
...onal program aimed at determining the three-dimens...determined more than 1,000 different structures. T...nction in many life processes and could lead to ta... PSI is a 10-year, approximately $600 million proj...neral Medical Sciences (NIGMS), part of the Nation...
Other Biology News:New research indicates a 'troubled' greenhouse is brewing 2Beyond lithium for bipolar disorder 2The Shapes Of Life: NIGMS Project Yields More Than 1,000 Protein Structures 2The Shapes Of Life: NIGMS Project Yields More Than 1,000 Protein Structures 3
Mouse/Rat Anti-Canine CD3 / CD4 Dual Colour Reagent Antibody, FITC / R-Phycoerythrin Conjugated from AbD Serotec
Rabbit anti-JLP AbVantage Pack Polyclonal Antibody, Unconjugated from Bethyl Laboratories
Protein Purification Applications from Promega
Essential Data: Gel Electrophoresis from Promega
Biology Products: