Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms ( Patrick M. Gaffney Eli...)

Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms

Patrick M. Gaffney, Elizabeth A. Orbacz, and Ziniu Yu, College of Marine Studies, University of Delaware, Lewes, Delaware.

Introduction
DNA polymorphisms are useful tools for ecological and evolutionary studies of both terrestrial and marine organisms, with applications ranging from species identification to delineation of population structure to monitoring genetic change in wild or domesticated populations. Denaturing gradient gel electrophoresis using the DCode system provides a convenient means of identifying genes with useful levels of polymorphism, and subsequently screening populations for variation at selected loci.

Unlike biomedical model organisms, most species of interest in ecological or evolutionary studies are not genetically wellcharacterized. Genes of interest are amplified by polymerase chain reaction* using universal primers targeting highly conserved regions.¹ Mitochondrial genes are popular candidates because of their typically rapid evolution, easy amplification, and sensitivity to reduced effective population size.² For DGGE, the haploid state of mtDNA offers two advantages. First, except for heteroplasmic individuals, the electrophoretic pattern of homoduplex DNA is simple. Secondly, the deliberate construction of heteroduplex molecules by pooling homoduplex PCR products often yields unique heteroduplex electrophoresis patterns, enabling identification of different haplotypes that cannot be separated on the basis of homoduplex mobility. We use DGGE to locate and score both nuclear and mitochondrial DNA polymorphisms in a variety of marine finfish and invertebrates, and describe a few examples here.

'"/>

Source:

Page: All 1 2 3 4 5 6 7 8
Related biology technology :

1. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
2. Oil-Free PCR Using the Hot Top Assembly
3. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
4. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
5. Using XL1-Red Mutator Strain to Generate Esterase Variants
6. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
7. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
8. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
9. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
10. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy

Post Your Comments:

(Date:5/20/2013)... CHAPEL HILL, N.C. , May 20, 2013 ... pharmaceutical company focused on developing differentiated antibiotics to ... bacterial infections, today announced that it presented data ... 2013) in Philadelphia demonstrating ... versus levofloxacin in Cempra,s prior Phase 2 clinical ...

(Date:5/20/2013)... WILMINGTON, Del. (PRWEB) May 20, 2013 ... named 14 young faculty members to its 2013 ... three years, the company will provide this outstanding ... their work in advancing basic science to meet ... is designed to help promising young and untenured ...

(Date:5/19/2013)... (PRWEB) May 19, 2013 Switzerland’s ... today announced a new speed record for a transatlantic ... Las Palmas, Spain, on April 25, 2013, the solar ... Ocean at the average speed of 5.3 knots before ... on May 18, 2013. The first-of-its-kind catamaran completed this ...

(Date:5/17/2013)... recipient,s immune system identifies the transplanted organ as foreign ... T cells, the immune cells that mediate rejection, must ... order to migrate to the transplanted organ. In this ... Fadi Lakkis and colleagues at the University of Pittsburgh ... cells is not required for migration. Instead, these cells ...

Breaking Biology Technology:Cempra Presents Post-Phase 2 Analysis of Solithromycin's Efficacy and Safety Results from Patients with Community Acquired Bacterial Pneumonia (CABP) 2 DuPont Celebrates Scientific Innovation by Recognizing Young Professors 2 DuPont Celebrates Scientific Innovation by Recognizing Young Professors 3 MS Tûranor PlanetSolar Sets World Speed Record for Transatlantic Crossing by Solar Electric Vessel 2 MS Tûranor PlanetSolar Sets World Speed Record for Transatlantic Crossing by Solar Electric Vessel 3 MS Tûranor PlanetSolar Sets World Speed Record for Transatlantic Crossing by Solar Electric Vessel 4 MS Tûranor PlanetSolar Sets World Speed Record for Transatlantic Crossing by Solar Electric Vessel 5 MS Tûranor PlanetSolar Sets World Speed Record for Transatlantic Crossing by Solar Electric Vessel 6

[0] Cempra Presents Post-Phase 2 Analysis of Solithromycin's Efficacy and Safety Results from Patients with Community Acquired Bacterial Pneumonia (CABP)[0] DuPont Celebrates Scientific Innovation by Recognizing Young Professors [0] MS Tûranor PlanetSolar Sets World Speed Record for Transatlantic Crossing by Solar Electric Vessel [0] Researchers identify signals that direct the immune system to reject a transplanted organ

... ... Spectrum Chemicals & Laboratory Products ... and decapper that will increase productivity and lessen injuries due ... advanced ergonomic design and is engineered to improve throughput in ...

... 10 deCODE genetics,(Nasdaq: DCGN ) today announced ... 2009. A conference call to discuss these results and,recent ... tomorrow,Tuesday, August 11, at 8:00am Eastern Daylight Time/12 noon ... The financial results presented below reflect deCODE,s strategic ...

... MONTREAL, Aug. 10 Enobia Pharma, an emerging biotech ... today announced that it raised US$50 million from its current ... used primarily to fund the ongoing development program for ENB-0040, ... and potentially deadly genetic bone disorder for which there is ...

Spectrum Announces New Distribution of AC-Powered Tool for Crimping and Decapping of Laboratory Vials - The CRIMPenstein™deCODE genetics Announces Second Quarter 2009 Financial Results Enobia Raises US$50M from Current Investors in Series C Financing

(Date:5/20/2013)... Boulder, Colo., USA New Geology ... 16 May 2013 cover a wide swath of ... tectonics, oceanography, geophysics, and paleobotany. Locations studied include ... mine at Alpi Apuane, Italy; Ukraine; Mars; and ... , 1. Rubies, jadeite, and plate tectonics;, ...

(Date:5/19/2013)... bacteria that can grow using hydrogen gas as its ... source of carbon. Researchers at the University of ... Meeting of the American Society for Microbiology. , "This ... hydrogen," says Amit Kumar, a researcher on the study ... Lovley Lab Group at the university. , Under the ...

(Date:5/18/2013)... MD (May 19, 2013) The AGA Research Foundation ... and Damian Augustyn Award in Digestive Cancer, which will ... of Medicine at the University of Pennsylvania, Philadelphia, as ... in pancreas development, regeneration and cancer progression. , "The ... in honor of two distinguished clinicians and AGA Legacy ...

[0] New in GEOLOGY: Gems, Darwin, Mars, Hemp, Snowball Earth, a Siberian Impact Crater, and More [0] Bacteria use hydrogen, carbon dioxide to produce electricity [0] AGA Research Foundation grant furthers digestive cancer research

... , Registration is now open for the Ecological Society ... in Portland, Oregon. The meeting is expected to draw more ... share emerging research. Jane Lubchenco, an Oregon State marine ... will open the six day meeting with a keynote address ...

... Combined, complimentary therapies have the ability to maximize ... spinal cord repair in rat models, according to a ... who published in a recent issue of Cell Transplantation ... "When transplanted, neural stem cells have demonstrated their ...

... April 17, 2012 /PRNewswire-iReach/ -- Global Information ... showing strong growth in the medical devices markets, driven ... Renal Dialysis Equipment Market to 2017 ... increasingly large elderly population are driving rapid growth in ...

Cached Biology News:Registration open for the Ecological Society of America's 2012 Annual Meeting in Portland, Ore. 2 Neural stem cell transplants for spinal cord injury maximized by combined, complimentary therapies 2 Global Surgical Devices Market Growth Driven by Improving Standards of Living and Longer Life Expectancies, Research Shows 2 Global Surgical Devices Market Growth Driven by Improving Standards of Living and Longer Life Expectancies, Research Shows 3 Global Surgical Devices Market Growth Driven by Improving Standards of Living and Longer Life Expectancies, Research Shows 4

Registration open for the Ecological Society of America's 2012 Annual Meeting in Portland, Ore.Neural stem cell transplants for spinal cord injury maximized by combined, complimentary therapies Global Surgical Devices Market Growth Driven by Improving Standards of Living and Longer Life Expectancies, Research Shows

... Safe Deposit Service ECACC is able to accept ... cGMP for cryo-storage. The cell lines must be ... been produced in accordance with the requirements of ... freedom from mycoplasma, bacteria or fungi. What are ...

Standard and modified oligonucleotides up to 150 nt-long at any scale for broad applications. Price starts from 30p/base (10 nmole scale)....

... of expertise in the field of Enzyme-Linked ... most powerful systems on the market. Freedom ... in-microplate tests requiring incubation and washing steps. ... (such as worktable shaker incubators, reader,washer), all ...

... Choice Microsphere/Microarray Stabilizer is a is a ... This product is an aqueous solution ... buffer (phosphate buffered saline), pH 6.6 - ... of 0.02% methylisothiazolone and 0.02% bromonitrodioxane as ...

Biology Products:

cGMP Safe Deposit Facility from European Collection of Cell Cultures (ECACC)Standard and Modified Oligonucleotides Service from Yorkshire Bioscience Ltd ELISA Biopharma from Tecan StabilGuard Choice Microsphere/Microarray Stabilizer from SurModics, Inc.