Navigation Links
Using the Agilent 2100 bioanalyzer for,,,quality control of protein samples prior to,,,MS-analysis

Whereas in the past mass spectrometers (MS) were confined to the realm of small molecules, the development of soft ionization methods such as matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) revolutionized the analysis of large biomolecules1, 2. Today, the analysis of proteins up to 300 kDa using MS has become a routine method to gain molecular weight information with high accuracy and structural information. However, sample purity greatly influences the results of MS analysis. In MS service facilities, scientists have to rely on the information provided by the customers and the quality of the sample preparation they have done. Bad results are often the consequence of customers being too optimistic about their own samples. Especially concentration and purity are often overestimated. To avoid unproductive MS runs, a fast and simple precheck is valuable. In this Application Note we show that the Agilent 2100 bioanalyzer can be used for quality control of protein samples prior to MS analysis. A corresponding data set analyzed with LC/MS and with the Agilent 2100 bioanalyzer using the Protein 200 Plus LabChip kit is shown.
Experiments
All samples were adjusted to a concentration of 1 mg/mL by dilution with the appropriate buffer prior to the analysis (based on information given by the customer).
LC-MS analysis
The experimental setup comprised:
Micro-HPLC-Pump (ABI 140C)
Autosampler (PE200)
HPLC column (Nucleosil C8 125 x 2 mm, 5 , 120 from Macherey & Nagel)
UV detector (ABI 785A) set to 214 nm, in row with
Single quadrupole mass spectrometer (API165 from Applied Biosystems) wi
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
2. Oil-Free PCR Using the Hot Top Assembly
3. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
4. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
5. Using XL1-Red Mutator Strain to Generate Esterase Variants
6. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
7. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
8. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
9. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
10. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
Post Your Comments:
(Date:1/22/2015)... PRINCETON, N.J. , Jan. 22, 2015  Derma ... tissue regeneration company focused on advanced wound care, ... from placental tissues, have been added to the ... announcement increases the opportunity for the AMNIOEXCEL® and ...
(Date:12/24/2014)... Texas , Dec. 24, 2014  Vermillion, Inc. ... on gynecologic disease, announced today that the Company ... including Oracle Investment Management, Jack W. Schuler ... Total proceeds were $10.5 million, before offering expenses.  ...
(Date:12/24/2014)... 23, 2014 Earlier this year in a ... of the Adult Stem Cell Technology Center, LLC ( ASCTC ... unique property of adult tissue stem cells. His title ... Important for the Future,” embodied the essence of his message ...
(Date:12/24/2014)... 2014 The report provides basic ... classification, application and industry overview. This report also ... structure. Production is separated by regions, technology and ... equipment, downstream client survey, marketing channels, industry development ...
Breaking Biology Technology:Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 2Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 3Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 4Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 5Vermillion Announces Closing of Equity Financing 2Vermillion Announces Closing of Equity Financing 3Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 2Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 3Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 4Worldwide Methyl Mercaptan Market 2015-2020 Forecasts on Development & Trends Now Available at ReportsnReports.com 2
... to Chief Financial Officer -, EXTON, Pa., ... today announced that it has appointed its new ... resources,clinical development and medical affairs. Charles Rowland has ... officer; Thomas Macnamara has,been promoted to the position ...
... Ltd, a cancer therapeutics,company, today announced the completion ... Bio*One Capital and new investor Aravis Venture.,Additional new ... by,Adamant Biomedical Investments AG and Zurcher Kantonalbank (ZKB). ... development efforts of its,two lead compounds, SB939, a ...
... via iMedica EHR provides patients with prescription ... DALLAS, Oct. 19 iMedica Corporation, a ... practices,announced today that it has partnered with ... ScriptGuide(R) to physician practices using,the iMedica Patient ...
Cached Biology Technology:ViroPharma Enhances Leadership to Support Global Growth and Expanding Business 2ViroPharma Enhances Leadership to Support Global Growth and Expanding Business 3ViroPharma Enhances Leadership to Support Global Growth and Expanding Business 4ViroPharma Enhances Leadership to Support Global Growth and Expanding Business 5S*BIO Completes US$26 Million Equity Financing 2S*BIO Completes US$26 Million Equity Financing 3S*BIO Completes US$26 Million Equity Financing 4iMedica Partners With LDM GROUP to Offer ScriptGuide to Physician Practice Customers 2iMedica Partners With LDM GROUP to Offer ScriptGuide to Physician Practice Customers 3
(Date:12/19/2014)... Research and Markets ( http://www.researchandmarkets.com/research/8kmfcn/iphone_5s ) has ... Sensor - Apple/AuthenTec TMDR92 & Sapphire - Technology Report" ... http://photos.prnewswire.com/prnh/20130307/600769 Following the acquisition of ... feature with the iPhone 5S. It is currently the ...
(Date:12/19/2014)...  23andMe, Inc., the leading personal genetics company, today announced the ... ancestry of individuals from across the United States ... four hundred years ago, the United States ... different continents. This study illuminates how American history and the ...
(Date:12/17/2014)... 2014 Research and Markets ( http://www.researchandmarkets.com/research/s6qzt5/samsung_galaxy_s5 ) ... S5 - Home Button Synaptics Fingerprint Sensor Technology Analysis" ... http://photos.prnewswire.com/prnh/20130307/600769 Based on a totally ... for the first time a fingerprint sensor in its ...
Breaking Biology News(10 mins):iPhone 5S Fingerprint Sensor - Apple/AuthenTec TMDR92 & Sapphire - Technology Report 223andMe Study Sketches Genetic Portrait of the United States 223andMe Study Sketches Genetic Portrait of the United States 323andMe Study Sketches Genetic Portrait of the United States 4Samsung Galaxy S5 - Home Button Synaptics Fingerprint Sensor Technology Analysis 2
... percent of the cells that shuttle sound to the brain are ... scientists know about how hearing works. Whether a rare, whisper-small second ... the brain and have a real role in processing sound has ... in the October 22 issue of Nature , a Johns ...
... Council,s Board on Life Sciences will hold a public ... discussions will explore the contributions the life sciences can ... the intersection of energy research and the life sciences, ... life sciences research can make to the food supply. ...
... have been discovered in the primate brain by a ... of physics at Penn State University and two neuroscientists ... the Massachusetts Institute of Technology (MIT). "This research ... been identified in recordings of neuron activity," Jin said. ...
Cached Biology News:Now hear this 2Time-keeping brain neurons discovered 2
Request Info...
Recombinant Mouse sTNF RII/TNFRSF1B...
One-step, microplate or cuvet, colorimetric, linear detection range 0.2 mg/dL to 20 mg/dL. Procedure: 20 min....
Mouse Wnt-11 Affinity Purified Polyclonal Ab...
Biology Products: