Navigation Links
Using THERMAL DESORPTION GAS CHROMATOGRAPHY for the identification of fragrance and other components in cosmetic products

Introduction

Cosmetic products such as soap, shampoo, lotions and creams are often scented with suitable compounds to improve their appeal. This application note describes an analytical method to identify the fragrance and other compounds contained within these cosmetic formulations.

Because of the solid or viscous nature of these formulations, direct sample injection into a gas chromatograph is not feasible and some form of analyte extraction is normally required. The approach taken in this work was to place a small amount of the sample in a thermal desorption tube and use an inert carrier gas to purge the volatile sample constituents into a cooled secondary trap. The secondary trap was then rapidly heated to desorb the collected components into a gas chromatographic column for separation and subsequent detection and identification by mass spectrometry.

This thermal desorption gas chromatography mass spectrometry (TD-GC-MS) technique may be fully automated using standard instrumentation and so provides a convenient and easy way of screening difficult sample matrices such as cosmetic products for constituent volatile compounds.

Sample Preparation

Approximately 120 mg of sample was placed within a glass wool plug in a PTFE thermal desorption tube insert as shown in Figure 1a. The flexible tube was squeezed to disperse liquid samples into the glass wool and so ensure an open pathway for carrier gas to flow through it.

The PTFE insert was then placed in an empty stainless steel desorption tube, as shown in Figure 1b, ready for analysis.

The following samples were examined:

  • Liquid soap
  • Body cream
  • Massage lotion
  • Face cream
  • Bathroom soap bar
  • Shampoo
  • Body lotion

Analytical conditions

Each sample tube was analyzed using TD-GC-MS under the conditions listed in Table 1.

Results and Discussion

Figure 2 shows a total ion chromatogram (TIC) obtained from the liquid soap sample. Despite the messy nature of this sample, clean chromatography has been obtained thus enabling easy data processing. The TurboMass software was used to examine the mass spectral data of each of the major components and to perform a library search to identify each of these. Figures 3, 5 and 7 show some typical mass spectra taken from the liquid soap chromatogram and Figures 4, 6 and 8 show the corresponding results from the spectral library search. Figures 9 to 15 show the total ion chromatograms, with annotated peak identities, for the remaining samples.

Conclusions

This note has described a simple means of identifying fragrance and other volatile compounds in cosmetic preparations without the need for tedious and lengthy sample extractions.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
2. Oil-Free PCR Using the Hot Top Assembly
3. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
4. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
5. Using XL1-Red Mutator Strain to Generate Esterase Variants
6. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
7. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
8. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
9. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
10. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/27/2016)... Raleigh, NC (PRWEB) , ... June 27, 2016 ... ... a mission to bring innovative medical technologies, services and solutions to the healthcare ... development and implementation of various distribution, manufacturing, sales and marketing strategies that are ...
(Date:6/24/2016)... 24, 2016  Regular discussions on a range of subjects ... the two entities said Poloz. Speaking at a ... , he pointed to the country,s inflation target, which ... "In certain areas ... have common economic goals, why not sit down and address ...
(Date:6/23/2016)... ... 2016 , ... Mosio, a leader in clinical research patient ... and Retention Tips.” Partnering with experienced clinical research professionals, Mosio revisits the hurdle ... and strategies for clinical researchers. , “The landscape of how patients receive and ...
(Date:6/23/2016)... SILVER SPRING, Md. , June 23, 2016 ... evidence collected from the crime scene to track the criminal ... sick, and the U.S. Food and Drug Administration (FDA) uses ... Sound far-fetched? It,s not. ... whole genome sequencing to support investigations of foodborne illnesses. Put ...
Breaking Biology Technology:
(Date:6/2/2016)... June 2, 2016 Perimeter Surveillance ... Unmanned Systems, Physical Infrastructure, Support & Other Service  ... visiongain offers comprehensive analysis of the global ... will generate revenues of $17.98 billion in 2016. ... Inc, a leader in software and hardware technologies for ...
(Date:5/12/2016)... DALLAS , May 12, 2016 ... has just published the overview results from the Q1 ... of the recent wave was consumers, receptivity to a ... wearables data with a health insurance company. ... choose to share," says Michael LaColla , CEO ...
(Date:4/26/2016)... LONDON , April 26, 2016 ... EdgeVerve Systems, a product subsidiary of Infosys (NYSE: ... a partnership to integrate the Onegini mobile security ... (Logo: http://photos.prnewswire.com/prnh/20151104/283829LOGO ) The ... enhanced security to access and transact across channels. ...
Breaking Biology News(10 mins):