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Uses and Applications of X-tremeGENE Q2 Transfection Reagent

(data not shown), even when the same vector was used under optimized conditions for both reagents.

In Jurkat cells , the X-tremeGENE Q2 Transfection Reagent also produced beta-Gal expression that was several-fold higher than competitor reagents L-2 and DC (Figure 2). In these experiments, the X-tremeGENE pM1-beta-Gal vector was used with each transfection reagent (in other experiments, the ratios of DNA to transfection reagents and the total amount of complex added per cell were optimized for each reagent)

.

Figure 2: Jurkat cells transfected with the X-tremeGENE Q2 Transfection Reagent showed higher levels of beta-Gal expression than currently recommended competitor reagents L-2 or DC. Cells (4x105/ well) were transfected with the X-tremeGENE pM1-beta-Gal vector (Roche Applied Science) in a 24-well plate. In each case, the ratios of DNA / transfection reagent and the amount of complex used in each well following optimization were those recommended by the suppliers. The level of reporter gene expression was determined 48 hours post transfection, using the beta-Gal Reporter Gene Assay Kit (Roche Applied Science, Cat. No. 1 758 241).

Note: The level of expression of a transfected gene will vary with the specific cell type, number of cells transfected, quantity of plasmid DNA used, ratio of transfection reagent to DNA, type of expression vector, cell culture medium, and the culture conditions.


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