Navigation Links
Uses and Applications of X-tremeGENE Q2 Transfection Reagent

(data not shown), even when the same vector was used under optimized conditions for both reagents.

In Jurkat cells , the X-tremeGENE Q2 Transfection Reagent also produced beta-Gal expression that was several-fold higher than competitor reagents L-2 and DC (Figure 2). In these experiments, the X-tremeGENE pM1-beta-Gal vector was used with each transfection reagent (in other experiments, the ratios of DNA to transfection reagents and the total amount of complex added per cell were optimized for each reagent)

.

Figure 2: Jurkat cells transfected with the X-tremeGENE Q2 Transfection Reagent showed higher levels of beta-Gal expression than currently recommended competitor reagents L-2 or DC. Cells (4x105/ well) were transfected with the X-tremeGENE pM1-beta-Gal vector (Roche Applied Science) in a 24-well plate. In each case, the ratios of DNA / transfection reagent and the amount of complex used in each well following optimization were those recommended by the suppliers. The level of reporter gene expression was determined 48 hours post transfection, using the beta-Gal Reporter Gene Assay Kit (Roche Applied Science, Cat. No. 1 758 241).

Note: The level of expression of a transfected gene will vary with the specific cell type, number of cells transfected, quantity of plasmid DNA used, ratio of transfection reagent to DNA, type of expression vector, cell culture medium, and the culture conditions.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. A Versatile Power Supply for All Electrophoresis Applications
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
4. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
5. Optimizing Büchi® Rotary Evaporator Applications
6. Optimizing Büchi® Rotary Evaporator Applications
7. Optimizing Bchi Rotary Evaporator Applications
8. Uses and Applications of FuGENE 6 Transfection Reagent
9. Uses and Applications of FuGENE 6 Transfection Reagent
10. Uses and Applications of FuGENE 6 Transfection Reagent
11. Uses and Applications of FuGENE 6 Transfection Reagent
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Uses and Applications tremeGENE Transfection Reagent

(Date:10/19/2014)... October 19, 2014 According to ... (Access Points; WLAN Controllers; Wireless gateways)]- Global Advancements, ... segments the global Outdoor Wi-Fi market with analysis ... report MarketsandMarkets also identifies the drivers and the ... the trends, opportunities and challenges. , Browse 65 ...
(Date:10/18/2014)... 2014 The Asia-Pacific Bio-based Advanced ... the bio-based advanced phase change material market in ... Browse through the TOC of the Asia-Pacific Bio-based ... an idea of the in-depth analysis provided. This ... the Asia-Pacific bio-based advanced phase change material market, ...
(Date:10/18/2014)... (PRWEB) October 18, 2014 The Asian ... in Asia with analysis and forecast of revenue. The ... to $712.3 million by 2018, at a CAGR of ... TOC of the Asian arthroscopic devices market, to get ... provides a glimpse of the segmentation of arthroscopic devices ...
(Date:10/18/2014)... The Asia-Pacific Liquid Crystal on Silicon (LCoS) display market ... with analysis and forecast of revenue. This market was ... to reach $380.0 million by 2018, at a CAGR ... the TOC of the Asia-Pacific LCoS display market report, ... This also provides a glimpse of the segmentation of ...
Breaking Biology Technology:Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 2Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 3Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 4The Asia-Pacific Bio-based Advanced Phase Change Materials Market is estimated to grow to $39.8 million by 2018 - New Report by MicroMarket Monitor 2The Asia-Pacific Bio-based Advanced Phase Change Materials Market is estimated to grow to $39.8 million by 2018 - New Report by MicroMarket Monitor 3The Asia-Pacific Bio-based Advanced Phase Change Materials Market is estimated to grow to $39.8 million by 2018 - New Report by MicroMarket Monitor 4The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 2The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 3The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 4The Asia-Pacific Liquid Crystal on Silicon (LCoS) display market is expected to reach $380.0 million by 2018 - New Report by MicroMarket Monitor 2The Asia-Pacific Liquid Crystal on Silicon (LCoS) display market is expected to reach $380.0 million by 2018 - New Report by MicroMarket Monitor 3
... Therapeutics Corporation today,announced that Blake M. Paterson M.D., ... in Bioscience Award from the Greater Baltimore Council.,Dr. ... nominees,submitted by businesses, bioscience advocates, higher education,institutions and ... Dr. Paterson has received this award from the ...
... March 18 Caliper Life,Sciences, Inc. (Nasdaq: ... the,Lehman Brothers Global Healthcare Conference in Miami, will ... EDT. Kevin Hrusovsky, President and CEO of,Caliper, will ... The live,webcast can be accessed at, http://cc.talkpoint.com/LEHM002/031808a_jw/default.asp?entity=Caliper . ...
... Three biotech CEOs and,the president of one ... by the Greater Baltimore Committee as winners of ... the 2008 Greater Baltimore Region Bioscience Awards are:, ... E. Askew, President & CEO, CSA,Medical, Inc. Askew,s ...
Cached Biology Technology:Blake M. Paterson MD, CEO and Co-founder of Alba Therapeutics, Wins Greater Baltimore Committee 2008 Leadership in Bioscience Award 2Three CEOs and University President Named Winners of Greater Baltimore Committee's 2008 Baltimore Region Bioscience Awards 2
(Date:10/15/2014)... pneumonia in female mice to an enzyme activated by ... more resistant to respiratory infections than males. Now, an ... to bacterial pneumonia in female mice is linked to ... show that this enzyme is ultimately activated by the ... team, lead by Professor Lester Kobzik at the Harvard ...
(Date:10/15/2014)... Rosenstiel Award for Distinguished Work in Biomedical Science by ... of genomic instability and its implications for the immune ... to win the Rosenstiel Award; the first, Rod McKinnon, ... the Charles A. Janeway Professor of Pediatrics and Professor ... at the Howard Hughes Medical Institute at Boston Children,s ...
(Date:10/14/2014)... discriminating thieves, prostate cancer tumors scavenge and hoard copper ... such avarice may be a fatal weakness. , ... kill prostate cancer cells by delivering a trove of ... diseased cells brimming with the mineral, leaving non-cancer cells ... already commercially available for other uses, could soon be ...
Breaking Biology News(10 mins):Brandeis awards 44th Rosenstiel Award to pioneering geneticist Fred Alt 2Prostate cancer's penchant for copper may be a fatal flaw 2
... issues, 100 percent fruit and vegetable juices do help reduce ... conclusion is the result of a European study designed to ... significant role in reducing risk for both cancer and cardiovascular ... are comparable in their ability to reduce risk compared to ...
... created from poultry feathers may add value to ... environmental problem of plastic waste. , According to ... million tons of non-biodegradable plastic waste ends up ... of biological systems engineering at Virginia Tech, is ...
... to process information more chaotically than has long been ... by scientists at the University of Bonn. The passing ... they show, occur exclusively at the synapses, i.e. the ... that the neurons release their chemical messengers along the ...
Cached Biology News:100 percent juices found as beneficial to health as fruits and vegetables 2Researcher examines polymers created with poultry feathers 2Brain works more chaotically than previously thought 2Brain works more chaotically than previously thought 3