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Uses and Applications of X-tremeGENE Q2 Transfection Reagent

Despite the wide spectrum of cell types that can be transfected using currently available transfection reagents, there are significant differences in the efficiency of DNA delivery into different types of cell. In certain cell types, such as HeLa, K-562, and Jurkat, the broad-spectrum transfection reagents available today usually provide relatively low transfection efficiency and low-level protein expression. The X-tremeGENE Q2 Transfection Reagent (X-Q2 Reagent) from Roche Applied Science was specially designed and optimized for high-efficiency gene delivery and high-level protein expression in HeLa, K-562, and Jurkat cells.

X-tremeGENE Q2 Transfection Reagent provides exceptional transfection efficiency in K-562 cells; approximately 20 - 30% of cells are transfected (Figure 1).



Figure 1: K-562 cells transfected with the X-tremeGENE Q2 Transfection Reagent and the X-tremeGENE pM1-beta-Gal vector (Roche Applied Science) show strong beta-galactosidase staining. Cells were plated in a 24-well plate (1 X 105 /well) and transfected with a complex of plasmid DNA (2 g) and X-tremeGENE Q2 Transfection Reagent (4 l). 48 hours post transfection, cells were stained for beta-Gal expression using the beta-Gal Staining Set from Roche Applied Science, Cat. No. 1 828 673.

The level of beta-galactosidase (beta-Gal) expression within each K-562 cell can vary as seen here from intense blue to light blue. The level of beta-Gal expression obtained with the X-tremeGENE Q2 Transfection Reagent was several-fold higher than the next best competitor reagent recommended for the transfection of K-562 cells
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