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Uses and Applications of FuGENE 6 Transfection Reagent

-100 l into each of 6 tubes as indicated below. Use sterile Eppendorf tubes or small sterile polystyrene tubes labeled:
  • A (100 l)
  • B (94 l)
  • C (100 l)
  • D (97 l)
  • E (97 l)
  • F (94 l), corresponding to the plate layout above.
  • Mix the room-temperature FuGENE 6 Reagent by tapping or briefly vortexing (1 second).
    Carefully add the FuGENE 6 Reagent to the serum-free medium as follows.
    • 6 l to Tube B
    • 3 l to Tube D (3:1 ratio)
    • 3 l to Tube E (3:2 ratio)
    • 6 l to Tube F (6:1 ratio)
  • Ensure the entire amount of reagent is delivered directly from the pipette tip into the serum-free medium. Avoid contact of the FuGENE 6 Transfection Reagent with the walls of the tube containing the serum-free medium. Avoid a FuGENE 6 Reagent layer on top of the serum-free medium. Once the FuGENE 6 Reagent is added, immediately tap, flick, or vortex for 1 second to ensure adequate mixing of the components.
  • Incubate at room temperature for 5 minutes.

  • Make the complex

    Add the DNA to each tube (see table below). The amount added will depend on the starting concentration of DNA.

    Tube DNA at 0.5 g/l DNA at 1 g/l DNA at 2 g/l
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    TAG: Uses and Applications FuGENE Transfection Reagent

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