Navigation Links
Uses and Applications of FuGENE 6 Transfection Reagent

Specific technique considerations

Co-transfections

First, verify that both of your DNA constructs work in your cell line. Also, determine the ratio of DNA to FuGENE 6 Reagent (for example, 1 g of DNA and 3 l FuGENE 6 Reagent) that provides good expression levels. Then, perform the transfection, ensuring the DNA / FuGENE 6 Reagent ratio takes into account the total amount of DNA from the two constructs. For example, in the above experiment the co-transfection of 1 g each of two carefully measured DNA constructs would require 6 l of FuGENE 6 Transfection Reagent (2 g DNA to 6 l of FuGENE 6 Transfection Reagent). FuGENE 6 Transfection Reagent is very gentle, so there will not be a cytotoxic effect when an excess of FuGENE 6 Transfection Reagent is used.

Using spinner flasks

The special components (e.g., Pluronic F-68) that are added to the spinner flask media for cell protection can interfere with transfection. To overcome this problem:

At the time of subculturing, resuspend the cells in medium that does not contain protective reagents, then perform the transfection in a flask or Petri dish for one hour as suggested in the package insert. For example, for 1 g DNA, use 3 l FuGENE 6 Reagent for each 100,000-200,000 cells. The cell concentration may be several times the concentration you will use for seeding. After allowing an hour for transfection, dilute into your normal suspension medium, then place it into the spinner flask.

Producing stable transfectants

Do not use a large number of plates, or plate fewer cells per plate. FuGENE 6 Reagent is so gentle and efficient that you will obta in many transfected cells. Use a good construct and good selective medium, and you will obtain a large number of clones.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. A Versatile Power Supply for All Electrophoresis Applications
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
4. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
5. Optimizing Büchi® Rotary Evaporator Applications
6. Optimizing Büchi® Rotary Evaporator Applications
7. Optimizing Bchi Rotary Evaporator Applications
8. Uses and Applications of X-tremeGENE Q2 Transfection Reagent
9. Uses and Applications of FuGENE 6 Transfection Reagent
10. Uses and Applications of FuGENE 6 Transfection Reagent
11. Uses and Applications of FuGENE 6 Transfection Reagent
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/4/2016)... ... , ... PBI-Gordon Corporation is pleased to announce Doug Obermann has been promoted ... at PBI-Gordon in February 1988, after finishing his masters in agronomy from Iowa State ... service to national product manager, to helping develop, name and launch many of the ...
(Date:5/3/2016)... India , May 3, 2016 /PRNewswire/ ... (DNA Chip (Genomics, Drug Discovery, Gene Expression) ... End user (Academics Institutes, Diagnostics Centers), Fabrication ... published by MarketsandMarkets, the market is expected ... from USD 7.63 Billion in 2015, growing ...
(Date:5/3/2016)... Boston, MA (PRWEB) , ... May 03, 2016 , ... ... ranked number 25 out of the state’s 76 fastest-growing private companies; a small percentage ... of Massachusetts, and ranked organizations on the percent change in revenue from 2012 to ...
(Date:5/3/2016)... (PRWEB) , ... May 03, 2016 , ... ... announced the addition of Dr. Nancy Gillett to its Board of Directors. Dr. ... she served as Corporate Executive Vice President and Chief Scientific Officer. A board-certified ...
Breaking Biology Technology:
(Date:3/23/2016)... March 23, 2016 ... Sicherheit Gesichts- und Stimmerkennung mit Passwörtern ... (NASDAQ: MESG ), ein führender Anbieter ... Unternehmen mit SpeechPro zusammenarbeitet, um erstmals dessen ... wird die Möglichkeit angeboten, im Rahmen mobiler ...
(Date:3/17/2016)... 2016 ABI Research, the leader in ... biometrics market will reach more than $30 billion ... 2015. Consumer electronics, particularly smartphones, continue to boost ... to reach two billion shipments by 2021 at ... , Research Analyst at ABI Research. "Surveillance is ...
(Date:3/11/2016)... March 11, 2016 http://www.apimages.com ) - ... Picture is available at AP Images ( http://www.apimages.com ) - ... be used to produce the new refugee identity cards. DERMALOG will ... at CeBIT in Hanover next week.   ... will be used to produce the new refugee identity cards. DERMALOG ...
Breaking Biology News(10 mins):