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Uses and Applications of FuGENE 6 Transfection Reagent

97 97 97 94 94 94 Add FuGENE 6 Transfection Reagent (l) 3 3 3 6 6 6

Add DNA (g)

0.5 1 2 1 2 3
  1. Gently tap the tubes. Mix thoroughly, but do not vortex.
  2. Incubate at room temperature for 15-45 minutes.
  3. Add each FuGENE 6 Transfection Reagent:DNA mixture to a 35-mm culture dish or one-well of a 6-well plate. Swirl the plates.
  4. If you increase the DNA concentration (e.g., in a co-transfection experiment), proportionally increase the amount of FuGENE 6 Transfection Reagent.
  5. If your cell line is not easily transfected by the above FuGENE 6 Transfection Reagent:DNA ratios, test a wider range of ratios, including 215 l FuGENE 6 Transfection Reagent per 12 g DNA, per 35-mm culture dish.
  6. If no transfection is observed, repeat the experiments with DOTAP or DOSPER Liposomal Transfection Reagent)
Amount of Transfection complex was too low In some systems, increasing the amount of both FuGENE 6 Transfection Reagent and DNA (more than ten fold higher than the recommended amounts), can continue to increase the level of protein expression. The very low cytotoxicity of FuGENE 6 Reagent permits both the FuGENE 6 Reagent and DNA levels to be tested at these high levels without adversely affecting cell viability.


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