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Uses and Applications of FuGENE 6 Transfection Reagent

Optimization Tips

Refer to the following table if you observe low transfection efficiency.

Possible Cause Recommendation Nucleic acids of poor quality or insufficient quantity Verify the amount or quality of nucleic acid:
  • Use only high-quality plasmid preparations with no contaminating nucleic acids that can be sufficiently quantitated in a photometer
  • Use DNA at a concentration of 0.022.0 g/l
  • Verify that the transfected plasmid construct contains appropriate promoters and other sequences required for protein expression in the cell line being transfected
  • Perform a control transfection experiment with a commercially available transfection-grade plasmid preparation (e.g., the -gal control vectors supplied with the Mammalian Expression Vectors for Epitope Tagging (Cat. No. 1 814 664).

Note: Endotoxins are reported to be cytotoxic to some very sensitive cell lines (e.g., Huh-7) and primary cultures. When using FuGENE 6 Transfection Reagent for many common cell types, it may be possible to use DNA that contains higher endotoxin levels.

Insufficient number of cells were used

Use adherent cells at 5080% confluency. FuGENE 6 Transfection Reagent was aliquotted and stored in a new container

Ensure the FuGENE 6 Transfection Reagent is stored in the original container. FuGENE 6 Transfection Reagent came into contact with plastic


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