Application of FuGENE 6 Transfection Reagent in Vector-based shRNA Experiments
RNA interference induced by siRNAs or shRNAs allows reliable down-regulation of
the expression of specific genes in human and other mammalian cells. While
siRNAs introduced into cells are used directly for transient gene silencing,
vectors that encode for the intracellular synthesis of shRNA are the most
suitable approach for long-term studies of gene function.
Delivery is one of the most critical steps in siRNA applications. Recent data
show the successful use of FuGENE 6 Transfection Reagent for the delivery of
vectors for shRNA experiments (Application Note available soon).
Removal of promyelocytic leukemia protein (PML) in NIH 3T3 cells
by PML-specific shRNA (P. Paddison & G. Hannon, unpublished results).
The references below describe the use of FuGENE 6
Transfection Reagent in RNAi applications.
Hemann MT, Fridman JS, Zilfou JT, Hernando E, Paddison PJ, Cordon-Cardo C,
Hannon GJ & Lowe SW (2003) An epi-allelic series of p53 hypomorphs created
by stable RNAi produces distinct tumor phenotypes in vivo.
Paddison PJ, Caudy AA, and Hannon GJ (2002) Stable Suppression of Gene
Expression by RNAi in Mammalian Cells.
Paddison PJ, Caudy AA, Bernstein E, Hannon GJ, Conklin DS (2002) Short hairpin
RNAs (shRNAs) induce sequence-specific silencing in mammalian cells.
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