( ...)Uses and Applications of FuGENE 6 Transfection Reagent

Application of FuGENE 6 Transfection Reagent in Vector-based shRNA Experiments

RNA interference induced by siRNAs or shRNAs allows reliable down-regulation of the expression of specific genes in human and other mammalian cells. While siRNAs introduced into cells are used directly for transient gene silencing, vectors that encode for the intracellular synthesis of shRNA are the most suitable approach for long-term studies of gene function.

Delivery is one of the most critical steps in siRNA applications. Recent data show the successful use of FuGENE 6 Transfection Reagent for the delivery of vectors for shRNA experiments (Application Note available soon).

Removal of promyelocytic leukemia protein (PML) in NIH 3T3 cells by PML-specific shRNA (P. Paddison & G. Hannon, unpublished results).

The references below describe the use of FuGENE 6 Transfection Reagent in RNAi applications.

Hemann MT, Fridman JS, Zilfou JT, Hernando E, Paddison PJ, Cordon-Cardo C, Hannon GJ & Lowe SW (2003) An epi-allelic series of p53 hypomorphs created by stable RNAi produces distinct tumor phenotypes in vivo.

Paddison PJ, Caudy AA, and Hannon GJ (2002) Stable Suppression of Gene Expression by RNAi in Mammalian Cells.

Paddison PJ, Caudy AA, Bernstein E, Hannon GJ, Conklin DS (2002) Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells.

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