Navigation Links
Uses and Applications of FuGENE 6 Transfection Reagent

Application of FuGENE 6 Transfection Reagent in Vector-based shRNA Experiments

RNA interference induced by siRNAs or shRNAs allows reliable down-regulation of the expression of specific genes in human and other mammalian cells. While siRNAs introduced into cells are used directly for transient gene silencing, vectors that encode for the intracellular synthesis of shRNA are the most suitable approach for long-term studies of gene function.

Delivery is one of the most critical steps in siRNA applications. Recent data show the successful use of FuGENE 6 Transfection Reagent for the delivery of vectors for shRNA experiments (Application Note available soon).



Removal of promyelocytic leukemia protein (PML) in NIH 3T3 cells by PML-specific shRNA (P. Paddison & G. Hannon, unpublished results).

The references below describe the use of FuGENE 6 Transfection Reagent in RNAi applications.

Hemann MT, Fridman JS, Zilfou JT, Hernando E, Paddison PJ, Cordon-Cardo C, Hannon GJ & Lowe SW (2003) An epi-allelic series of p53 hypomorphs created by stable RNAi produces distinct tumor phenotypes in vivo.

Paddison PJ, Caudy AA, and Hannon GJ (2002) Stable Suppression of Gene Expression by RNAi in Mammalian Cells.

Paddison PJ, Caudy AA, Bernstein E, Hannon GJ, Conklin DS (2002) Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells.


'"/>

Source:


Page: All 1

Related biology technology :

1. A Versatile Power Supply for All Electrophoresis Applications
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
4. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
5. Optimizing Büchi® Rotary Evaporator Applications
6. Optimizing Büchi® Rotary Evaporator Applications
7. Optimizing Bchi Rotary Evaporator Applications
8. Uses and Applications of X-tremeGENE Q2 Transfection Reagent
9. Uses and Applications of FuGENE 6 Transfection Reagent
10. Uses and Applications of FuGENE 6 Transfection Reagent
11. Uses and Applications of FuGENE 6 Transfection Reagent
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/8/2016)... R&D program for the development of future natural products for ... research and development center in Israel ... Stockton has a variety of products adapted ... active in more than 35 counties worldwide. ... flagship product Timorex Gold ® is used to control ...
(Date:2/5/2016)... Feb. 5, 2016 ATCC, the premier global ... assist the medical and life science researchers that are ... infection.   CDC website . ... Zika virus is a single-stranded RNA virus of the ... Nile, Dengue and Chikungunya Viruses. Zika virus is transmitted ...
(Date:2/4/2016)... CA (PRWEB) , ... February 04, 2016 , ... ... enterprise talent development and compliance training, today announced an interactive FDA compliance ... Playbook™. The RAPS (Regulatory Affairs Professional Society) accredited interactive course on Morf ...
(Date:2/4/2016)... -- Beike Biotechnology, the Shenzhen ... in late 2015 to mark their successful combined efforts ... --> --> The signing, ... Therapy" was hosted by the Shenzhen Cell Bank and ... Beike Biotechnology Co., Ltd. Shenzhen,s ...
Breaking Biology Technology:
(Date:1/28/2016)... SYNA ), a leading developer of human interface solutions, today ... --> --> Net revenue ... to the comparable quarter last year to $470.5 million. Net income ... $0.93 per diluted share. --> ... 2016 grew 9 percent over the prior year period to $60.3 ...
(Date:1/22/2016)... January 22, 2016 ... addition of the  "Global Behavioral Biometric ... --> http://www.researchandmarkets.com/research/4lmf2s/global_behavioral ) has ... Behavioral Biometric Market 2016-2020"  report to ... and Markets ( http://www.researchandmarkets.com/research/4lmf2s/global_behavioral ) has announced ...
(Date:1/20/2016)... , Jan. 20, 2016   MedNet Solutions , ... entire spectrum of clinical research, is pleased to announce ... significant achievements are the result of the company,s laser ... iMedNet eClinical , it,s comprehensive, easy-to-use and highly ... --> Key MedNet growth achievements in 2015 ...
Breaking Biology News(10 mins):