Navigation Links
Uses and Applications of FuGENE 6 Transfection Reagent

e experiment with a secreted reporter gene assay such as SEAP, hGH, or a standard -gal control vector. Cells secreting SEAP should show little to no evidence of cytotoxicity.

The culture may be contaminated with mycoplasma

Use the Mycoplasma Detection Kit (Cat. No. 1 296 744) or Mycoplasma PCR ELISA (Cat. No. 1 663 925) to determine if the culture is contaminated. Treat the cells with BM Cyclin (Cat. No. 799 050) to eliminate the mycoplasma. Alternatively, start the transfections over with a fresh uncontaminated culture.

Cells may not be healthy (e.g., malfunctioning incubator, media problems)

Assess the physiological state of the cells and incubation conditions (e.g., CO2 and temperature levels). Perform the same controls as suggested above for cytotoxicity to eliminate the influence of transfection reagent or nucleic acid. Plasmid preparation
contaminated with large amounts of endotoxin


Endotoxin is reported to be cytotoxic to some very sensitive cell lines (e.g., Huh-7) and primary cultures. By using FuGENE 6 Transfection Reagent for many common cell types, it may be possible to use DNA containing higher endotoxin levels. If above tests prove negative, FuGENE 6 Transfection Reagent may be cytotoxic to your specific cell type If you are using a very sensitive cell line, measures can be taken to minimize cytotoxicity:
  • Perform the transfection in the presence of FBS
  • Reduce the time of exposure to the transfection reagent:DNA complex to 23 h, then replace the medium
  • Perform the transfection at a highe
    '"/>


Source:


Page: All 1 2 3

Related biology technology :

1. A Versatile Power Supply for All Electrophoresis Applications
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
4. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
5. Optimizing Büchi® Rotary Evaporator Applications
6. Optimizing Büchi® Rotary Evaporator Applications
7. Optimizing Bchi Rotary Evaporator Applications
8. Uses and Applications of X-tremeGENE Q2 Transfection Reagent
9. Uses and Applications of FuGENE 6 Transfection Reagent
10. Uses and Applications of FuGENE 6 Transfection Reagent
11. Uses and Applications of FuGENE 6 Transfection Reagent
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Uses and Applications FuGENE Transfection Reagent

(Date:7/24/2014)... 2014 Gain recognition for leadership ... of the biotech industry. Nominations are now being ... to you by the Bio Supply Management ... chain management professionals for the past 7 years. ... the Biotech industry – Manufacturers, Service Providers, Material ...
(Date:7/24/2014)... , July 24, 2014  Asterias Biotherapeutics, Inc. ... emerging field of regenerative medicine, announced today that ... presentation to investors on Tuesday, July 29, 2014, ... presentation will include an overview of Asterias, business ... webcast, visit http://www.ustream.tv/channel/asterias-biotherapeutics at least 15 ...
(Date:7/24/2014)... trained personnel, and detection dogs to safeguard airports ... revolutionary new electronic chip with nano-sized chemical sensors ... , The groundbreaking nanotechnology-inspired sensor, devised by Prof. ... of Chemistry and Center for Nanoscience and Nanotechnology, ... up the scent of explosives molecules better than ...
(Date:7/24/2014)... scientists are making progress in devising suitable means ... relies on quantum dotsa kind of artificial atom, ... new study demonstrates that changing the coupling of ... impulses can help better control them. This has ... quantum information units, which would produce faster quantum ...
Breaking Biology Technology:Call for Submission: BSMA’s Supply Chain Management Innovation Awards 2Call for Submission: BSMA’s Supply Chain Management Innovation Awards 3Asterias Biotherapeutics Announces Live Investor Webcast 2Asterias Biotherapeutics Announces Live Investor Webcast 3Asterias Biotherapeutics Announces Live Investor Webcast 4Nano-sized chip 'sniffs out' explosives far better than trained dogs 2
... Yang, Associate Professor of Mechanical Engineering and Director of ... Technology, The Innovation University (TM), will receive funding from ... a Nanoimprint Lithography System (NIL) for the purpose of ... Stevens. The Co-PIs of the project are Drs. Besser, ...
... Cumberland Pharmaceuticals Inc. (Nasdaq: CPIX) ... the safety and efficacy of Caldolor® ( ibuprofen ) ... ill adults. Published in the peer-reviewed journal Critical ... significantly more effective at reducing fever in hospitalized patients ...
... Castle Access, Inc. announces the appointment of ... the Castle Access organization with that of its ... provider, and gives clients in Southwestern US and ... data center and mission-critical managed services. ...
Cached Biology Technology:Nanoimprint lithography NSF grant awarded to micro device lab at Stevens 2Nanoimprint lithography NSF grant awarded to micro device lab at Stevens 3Newly Published Caldolor® Clinical Data Demonstrates 77% Reduction in Fever in Hospitalized Patients 2Newly Published Caldolor® Clinical Data Demonstrates 77% Reduction in Fever in Hospitalized Patients 3Newly Published Caldolor® Clinical Data Demonstrates 77% Reduction in Fever in Hospitalized Patients 4Castle Access Names New Managing Director 2Castle Access Names New Managing Director 3
(Date:7/25/2014)... A study published online in the International ... novel approach to preventing cervical cancer based on findings ... after removal of a discrete population of cells in ... study that looked at squamocolumnar junction cells, or SCJ ... have been implicated as the origins of cervical cancer. ...
(Date:7/25/2014)... of Adelaide has opened the way for the development of ... In Australia, annual barley production is second only to wheat ... of the most important diseases of barley. , Senior Research ... of special growths on the cell walls of barley plants ... , The research, by the ARC Centre of Excellence in ...
(Date:7/25/2014)... that the age girls reach puberty is influenced by ,imprinted ... parent contributes the gene. This is the first evidence ... birth and details of this study were published today in ... period, known as menarche, is a marker for the timing ... onset of menses varies between girls, is an inherited trait, ...
Breaking Biology News(10 mins):Clearing cells to prevent cervical cancer 2New hope for powdery mildew resistant barley 2Could age of first period influence development of diseases in older women? 2
... reveals critical molecular events in the origin of fat ... such as obesity and diabetes, as fat cells produce ... The study finds that a hormonal cocktail routinely used ... the transition from fat-cell precursors to full-blown fat, researchers ...
... study reveals critical molecular events in the origin of ... diseases, such as obesity and diabetes, as fat cells ... , The study finds that a hormonal cocktail routinely ... in the transition from fat-cell precursors to full-blown fat, ...
... how a common fish found along the West Coast can ... The study marks the first time that scientists have found ... vertebrate's brain that controls the vocal muscle system and the ... that understanding the auditory system of the plainfin midshipman fish ...
Cached Biology News:Key To Stem Cell Transplant Success Is Tricking Immune System 2Short-term Bio Sensors Monitor From Afar 2How fish hear and make sounds at same time 2
... , CapSure HS and CapSure Macro LCM ... of populations of pure cells from tissue ... Microdissection., Ensure the Integrity of Microdissected ... CapSure LCM Caps have a patented transfer ...
... MyiQ real-time PCR detection system offers an ... green fluorescent dyes such as FAM and ... with the iCycler thermal cycler, offering superior ... driven performance. The MyiQ real-time PCR detection ...
Opticon continuous fluorescence detector; includes optical base, analysis software, computer and monitor (this is an accessory for the DNA Engine thermal cyclers which must be ordered seperately)...
... are highly sensitive laser confocal systems designed ... advanced scanners that meet your expectations, differentiating ... and optimizing the signal on the bottom ... the range for increased sensitivity. Results with ...
Biology Products: