Navigation Links
Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A

r constant voltage conditions, every gel experiences the same running conditions regardless of the number of gels in the tank. The temperature did not rise during the run and the buffer recirculation seemed to be particularly efficient in maintaining a uniform temperature throughout the inner buffer chamber.

Staining and Imaging
Post-electrophoresis, the gels were removed from the PROTEAN Plus hinged spacer plates. Care needs to be taken while removing 1.0 mm thick gels from their cassettes, as they are fragile and easily torn. Thicker gels would be easier to handle (Bio-Rads 1.5 mm cassettes are recommended, catalog #165-4171 for 20 x 20.5 cm and catalog #165-4174 for 25 x 20.5 cm cassette sizes). Ten of the gels were stained with colloidal Coomassie Blue for 48 hr and restained for a further 48 hr with fresh stain; one gel was stained with SYPRO Ruby protein gel stain (image not shown). The gels were destained with and stored in water. Gel images were acquired on an Agfa Arcus II scanner through a single thickness of red document wallet. The images were channel split in Microsoft Paintshop Pro software so that the red channel of the RGB TIFF file was selected.


Results and Discussion
Images of the 10 Coomassie Blue-stained gels run on the PROTEAN Plus Dodeca cell are shown in Figure 2. The gels are of excellent quality and are very consistent with the exception of some minor differences due to the isoelectric focusing step. The highly expressed proteins did not suffer from vertical streaking and the other proteins all show welldefined spots (see Figure 3). These high-quality gels are excellent for image analysis comparisons and were sufficiently loaded to a
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Separation Reproducibility With the BioLogic Chromatography Systems
2. Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A
3. Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System
4. Separation and Quantitation of R and S Enantiomers using Normal Phase Chromatography on an API 3000 LC/MS/MS System
5. BD IMag Human Regulatory T Lymphocyte Separation Set for Isolation of Treg Cells
6. Separation of Peptides by Hydrophilic Interaction Chromatography Using TSKgel Amide-80
7. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
8. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
9. Performance Comparisons of Commercial RT-PCR Systems
10. A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7 Cell Lines
11. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Use the PROTEAN Plus Dodeca Cell for Second Dimension SDS PAGE Rev

(Date:8/28/2015)... ... August 28, 2015 , ... Since the initial launch of FireflySci Inc. (FFS) in early ... excellent customer service. Images such as the Fonz in a lab coat and Large ... to the humdrum spectroscopy field. , FireflySci is proud to be the only ...
(Date:8/27/2015)... , Aug. 27, 2015 The National ... patient,s eyes—and life—were saved by doctors at Vanderbilt University ... with NNFF, NovaBay Pharmaceuticals and Dr. John Crew ... at Seton Medical Center in Daly ... used a breakthrough approach, pioneered by Dr. Crew, that ...
(Date:8/27/2015)... ... 27, 2015 , ... Inc. Magazine released its annual list of the 5,000 ... in growth for the three years through 2014. Being named to the Inc. 5000 ... in the country. , “We are thrilled to make the Inc. 5000 list ...
(Date:8/26/2015)... 26, 2015 /PRNewswire/ - iCo Therapeutics ("iCo" or "the ... results for the quarter ended June 30, 2015. ... dollars and presented under International Financial Reporting Standards ... respect to our Oral Amp B program, rapidly ... said Andrew Rae , President & CEO ...
Breaking Biology Technology:Cuvette Manufacturer FireflySci Launches Back to School Deals with Complimentary Shipping On All Orders 2Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 2Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 3iLab Solutions Named to 2015 Inc. 5000 List 2iLab Solutions Named to 2015 Inc. 5000 List 3iCo Therapeutics Announces Second Quarter 2015 Financial Results 2iCo Therapeutics Announces Second Quarter 2015 Financial Results 3
... CAMBRIDGE, Mass., Nov. 23, 2011 /PRNewswire/ -- ... the second of the company,s three-tranche $91 million financing ... tranche included $21.4 million in equity financing from Radius, ... GE Capital, Healthcare Financial Services and Oxford Finance LLC, ...
... hole would prevent light from going through it, but Princeton University ... has found that placing a metal cap over a small hole ... but rather enhances its transmission. In an example of the ... electrical engineer Stephen Chou and colleagues made an array ...
... 22, 2011 Molecular diagnostics company GenturaDx announced ... the appointment of three initial members: Dr. Keith ... and Dr. Gregory Tsongalis, Ph.D., H.C.L.D., F.A.C.B. The ... medicine and the commercial market related to initial ...
Cached Biology Technology:Radius Closes $27.65 Million Second Tranche of Previously Announced Financing 2Radius Closes $27.65 Million Second Tranche of Previously Announced Financing 3Blocked holes can enhance rather than stop light going through 2Blocked holes can enhance rather than stop light going through 3GenturaDx Announces Formation of Scientific Advisory Board 2
(Date:7/31/2015)... juli 2015 Den 10. internationale konference om ... den 22. - 25. oktober i Shenzhen ... 10-års fødselsdag i år. Siden starten i 2006 er ... på ,omik,-feltet, og er en af de mest dynamiske, ... fokuserer på nylige gennembrud og fremskridt inden for ,omik,-forskning, ...
(Date:7/31/2015)... de julio de 2015 La 10 th ... por medio de BGI del 22 al 25 de octubre de ... Este año, la conferencia celebra su décimo aniversario. Desde ... en una de las reuniones anuales más influyentes del mundo ... reuniones más dinámicas, entusiastas y mejores a nivel científico. ...
(Date:7/30/2015)... , July 30, 2015 Cellecta, ... for gene function analysis and biomarker discovery, announced ... (sgRNA) Knockout Library targeting all human protein coding ... permanently "knock out" a gene,s function. Cellecta,s new ... high throughput screening tool so that researchers can ...
Breaking Biology News(10 mins):Den 10. internationale konference om Genomics (ICG-10) åbner i oktober 2La 10th International Conference on Genomics (ICG-10) se llevará a cabo en octubre 2La 10th International Conference on Genomics (ICG-10) se llevará a cabo en octubre 3CELLECTA, INC. Announces Launch of Human Whole Genome CRISPR Knockout Library 2
... a land owner and animals such as coyotes or wild pigs ... way to control their numbers in a humane way in ... at Texas A&M Universitys College of Veterinary Medicine & Biomedical Sciences. ... baited food, researchers say. Researchers are testing oral contraceptives ...
... at the center of some of the nation,s most ... global climate change. To provide a critical overview of ... summit of leading energy experts from the government, research ... an Academies study under way called "America,s Energy Future: ...
... syndrome (FXS) is the most common hereditary form of ... attention deficit and/or hyperactivity disorder (ADHD), which complicates social ... such as Ritalin is often successfully used to treat ... is effective in children with mental retardation, it also ...
Cached Biology News:Texas A&M testing oral contraceptives for animals 2NAS to hold 2-day summit on energy issues 2Effective ADHD treatment found for children with fragile X syndrome 2
... Reagents for the differentiation of mouse ... cell bodies exist in the midbrain, where ... nigra, the ventral tegmental area, and the ... rise to extensive forebrain dopamine innervation ( ...
... aptamer screening and binding optimization and built ... on-chip synthesis platform. Thousands of customer specified ... Sciences can provide assistance with custom sequence ... of our comprehensive DNA/RNA Aptamer Microarray Service. ...
... of Parkers MX80 family of positioners, offering longer ... hole patterns mimic the MX80 family, allowing users ... brackets to build multi-axis systems., , Miniature ... , Accelerations to 5 gs , Velocities to ...
... Kit with Medium High quality ready-to-use cryopreserved ... base medium for growth and lineage commitment. ... generated from E18 fetal rat brain in ... mixture of in vitro stem/progenitor cells, neurons, ...
Biology Products: