Navigation Links
Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A

r constant voltage conditions, every gel experiences the same running conditions regardless of the number of gels in the tank. The temperature did not rise during the run and the buffer recirculation seemed to be particularly efficient in maintaining a uniform temperature throughout the inner buffer chamber.

Staining and Imaging
Post-electrophoresis, the gels were removed from the PROTEAN Plus hinged spacer plates. Care needs to be taken while removing 1.0 mm thick gels from their cassettes, as they are fragile and easily torn. Thicker gels would be easier to handle (Bio-Rads 1.5 mm cassettes are recommended, catalog #165-4171 for 20 x 20.5 cm and catalog #165-4174 for 25 x 20.5 cm cassette sizes). Ten of the gels were stained with colloidal Coomassie Blue for 48 hr and restained for a further 48 hr with fresh stain; one gel was stained with SYPRO Ruby protein gel stain (image not shown). The gels were destained with and stored in water. Gel images were acquired on an Agfa Arcus II scanner through a single thickness of red document wallet. The images were channel split in Microsoft Paintshop Pro software so that the red channel of the RGB TIFF file was selected.


Results and Discussion
Images of the 10 Coomassie Blue-stained gels run on the PROTEAN Plus Dodeca cell are shown in Figure 2. The gels are of excellent quality and are very consistent with the exception of some minor differences due to the isoelectric focusing step. The highly expressed proteins did not suffer from vertical streaking and the other proteins all show welldefined spots (see Figure 3). These high-quality gels are excellent for image analysis comparisons and were sufficiently loaded to a
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Separation Reproducibility With the BioLogic Chromatography Systems
2. Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A
3. Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System
4. Separation and Quantitation of R and S Enantiomers using Normal Phase Chromatography on an API 3000 LC/MS/MS System
5. BD IMag Human Regulatory T Lymphocyte Separation Set for Isolation of Treg Cells
6. Separation of Peptides by Hydrophilic Interaction Chromatography Using TSKgel Amide-80
7. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
8. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
9. Performance Comparisons of Commercial RT-PCR Systems
10. A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7 Cell Lines
11. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Use the PROTEAN Plus Dodeca Cell for Second Dimension SDS PAGE Rev

(Date:11/21/2014)... 2014 /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ("Biorem" or "the Company") today ... September 30, 2014.  Biorem,s complete 2014 third quarter financial statements and ... Financial Summary:Three-months ended September 30, , Nine-months ended ... , 2014 , 2013 , 2014 ... 5,281 , 6,715 , 14,476 Gross profit ...
(Date:11/18/2014)... RPS Diagnostics (RPS®) – a ... announces its third annual partnership of the Centers ... About Antibiotics Week from November 17-23. , Get ... designed to highlight the coordinated efforts of CDC, ... for-profit partners to provide education about the importance ...
(Date:11/18/2014)... Urdorf, Switzerland (PRWEB) November 17, 2014 ... range of processes across the pharmaceutical, chemical, and food ... is it so important, and how do pH sensors ... measurement to assist those in process industries with these ... description of pH measurement in the process industries. The ...
(Date:11/18/2014)... (PRWEB) November 18, 2014 Alanda ... and Transparency Reporting solutions, announced today that a ... using its Consummate Providerâ„¢ solution in both the ... a Global, SaaS based, Data Quality remediation portal, ... their third party Providers improve their ability to ...
Breaking Biology Technology:Biorem Reports Third Quarter Results 2Biorem Reports Third Quarter Results 3RPS Again Partners with the CDC in Support of the 2014 Get Smart About Antibiotics Week 2RPS Again Partners with the CDC in Support of the 2014 Get Smart About Antibiotics Week 3pH Guide from METTLER TOLEDO Explains Measurement, Theory and Practice of pH Applications 2Alanda Software Completes Successful Implementation of Global Data Quality & Vendor Management Solution for Top Tier Global Biopharmaceutical Company 2
... Ontario, Nov. 6 Helix BioPharma Corp. (TSX, FSE: "HBP" ... and chief operating officer, will present at Merriman Curhan Ford,s ... PM at the Sofitel Hotel in New York City. ... product development programs L-DOS47 and Topical Interferon Alpha-2b. The ...
... - Oncolytics Biotech Inc. (TSX:ONC, NASDAQ:ONCY) ("Oncolytics" or the ... study of intravenous REOLYSIN(R) in patients with sarcomas metastatic ... 15th Annual Connective Tissue Oncology Society Meeting held in ... poster presentation, entitled "A Phase II Study of Intravenous ...
... The endangered black-footed ferrets inhabit prairie dog towns in the ... The prairie dogs are very susceptible to plague ... U.S. Fish & Wildlife Service (FWS) has implemented a ferret ... their formerly native habitat. Unfortunately, plague can sweep through ...
Cached Biology Technology:Helix BioPharma to Present at Merriman Curhan Ford's Investor Summit 2009 on November 10th 2Helix BioPharma to Present at Merriman Curhan Ford's Investor Summit 2009 on November 10th 3Oncolytics Biotech(R) Inc. Collaborators Present Positive Phase II Sarcoma Trial Results at CTOS Annual Meeting 2Oncolytics Biotech(R) Inc. Collaborators Present Positive Phase II Sarcoma Trial Results at CTOS Annual Meeting 3Colorado Companies Team Up With U.S. Fish & Wildlife Service to Protect Black-Footed Ferret 2
(Date:11/7/2014)... BURBANK, Calif. , Nov. 7, 2014  In conjunction ... Burbank Police Departments, The Community Foundation of the ... BURBANK DNA JUSTICE PROJECT," a $250,000 initiative to ... successful initial phase. In its first six months of existence, ... desperately needed evidence support for local law enforcement. ...
(Date:11/6/2014)... Insilico Medicine, Inc, a Baltimore-based bioinformatics company focused ... announced a research collaboration with the international leader ... (OTC: CSBR). , "There are many companies utilizing ... Oncology,s TumorGraft technology is unique in a way ... generating vast amounts of valuable data. Our bioinformatic ...
(Date:11/4/2014)... In nature, the right amount of death at the ... density, according to new research that could help in ... wildlife stocks. , In a paper in the journal ... University researcher and European colleagues conclude that the kind ... a loss of individuals, or mortality, depends on the ...
Breaking Biology News(10 mins):Community Foundation Of The Verdugos Unveils Campaign To Fund Glendale-Burbank DNA Justice Project 2Insilico Medicine, Inc announces research collaboration with Champions Oncology, Inc 2When less is more: Death in moderation boosts population density in nature 2
... Stephen Moose has developed a corn plant with enormous potential ... silage, Moose said, due to a greater number of leaves ... energy crop. The gene known as Glossy 15 was ... waxy coating that acts like a sun screen for the ...
... for its health benefits. But now scientists have ... produce medicines for several autoimmune and inflammatory diseases, ... open access journal BMC Biotechnology . ... research organizations have participated in the study as ...
... knows, children love sweet-tasting foods. Now, new research ... indicates that this heightened liking for sweetness has a ... rate. "The relationship between sweet preference and growth ... demands increase. Children are programmed to like sweet ...
Cached Biology News:Doubling a gene in corn results in giant biomass 2Tobacco makes medicine 2Liking sweets makes sense for kids 2
Kit containing MACS equipment and reagents for immunopurification of proteins....
... LCMSsolution software offers an intuitive, ... instrument control, diverse data handling, ... requirements. Comprehensive functions include data ... data management in both standalone ...
... a modern flow cytometer, a scientist can ... labeled with fluorescent probes. However, the emission ... cell labeling are broader than the light ... filters. Thus, the fluorescence signal of a ...
... Sigmas FLAG Immunoprecipitation kit allows a ... of an active FLAG-tagged protein. The ... gel, which is a highly specific ... resin. The use of affinity resin ...
Biology Products: