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Use of the HitHunter EFC Chemiluminescent Detection Kits with LEADseeker Multimodality Imaging System

Key words: EFC chemiluminescence • HitHunter • LEADseeker


Competitive pressure is forcing organizations to continually increase screening throughput. Yet accuracy and sensitivity remain vital concerns, particularly for common screens such as luminescence assays. LEADseeker™ Multimodality Imaging System was developed to meet these demands by combining excellent detection with the ability to image whole plates in less than one minute.

This application note describes the use of the HitHunter™ range of EFC chemiluminescent assays with the LEADseeker Multimodality Imaging System. The Caspase 3 and p38 MAPK binding assay kits were used as model systems. The data demonstrate that the HitHunter range of assay kits, which are based on enzyme fragment complementation technology, perform well in conjunction with the chemiluminescent readout of LEADseeker Multimodality Imaging System equipped with the supplied luminescence filter. Used together, the components support organizations interested in adding high and ultrahigh throughput chemiluminescent assays to their screening programs.

Materials
Products used
LEADseeker Multimodality Imaging System 18-1140-71

HitHunter Caspase 3 EFC Chemiluminescent 90-0028-02
Detection Kit

HitHunter p38 MAPK Binding Assay EFC 90-0028-31(L)
Chemiluminescent Detection Kit


Other materials required
Luminescence 660 nm cut-off filter

384-well plates (non-treated, non-sterile, polystyrene, white)

DEVD-CHO inhibitor (BIOMOL)

SB203580 and SB239063 inhibitors (Calbiochem)


Method
1. Caspase
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Source:


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