Navigation Links
Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

es, Hercules, CA) was added to the agarose solution at a concentration of 0.5 mg/ml before the gel was poured in the mold. The gel was run for 1 hr at 50 V, and the PCR product was visualized under UV-light. The amplified products were stored at -20 C until they were used for DGGE.

DGGE
DGGE was performed with a DCode denaturing gel electrophoresis system (Bio-Rad Laboratories, Hercules, CA). A parallel gel was prepared with a denaturing gradient ranging from 15 to 40% as described in Appendix 1 (where 100% denaturant contained 7 M urea (Bio-Rad Laboratories, Hercules, CA) and 40% formamide (Fisher Scientific, Pittsburgh, PA). The gel also contained a gradient of polyacrylamide ranging from 10 to 20%, made from a 40% stock solution of acrylamide/bisacrylamide (37.5:1) (Bio-Rad Laboratories, Hercules, CA). The gel was cast using a Bio-Rad Model 385 Gradient Delivery System (Bio-Rad Laboratories, Hercules, CA) and a non-denaturing, 6% acrylamide loading gel (Appendix 1) was cast on top of the gradient gel. The gel was placed in 0.5x strength Tris/Acetic Acid/EDTA buffer (TAE) (Bio-Rad Laboratories, Hercules, CA) equilibrated to 60 C. Five l PCR product was mixed with 5 l loading buffer and applied to the gel wells. Electrophoresis was carried out at 60 C for 15 h at 200 V. The gel was subsequently soaked for 30 min in 1/10,0000x strength SYBR Green I nucleic acid gel stain (Molecular Probes, Inc., Eugene, OR) and visualized under UV-light.


Results and Discussion
Primer pair 1055F and1406R with GC-clamp yielded a single band on agarose gel corresponding to a 400 bp DNA fragment when the PCR reaction was carried out with Pfu polymerase on DNA extracted from L. m
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13

Related biology technology :

1. DGGE Analysis Using the DCode System, Rev B
2. Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System
3. Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System
4. Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System
5. Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System
6. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
7. TTGE on the DCode System
8. Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System
9. Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms
10. Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System
11. Detection of Mutations in the CYP21 Gene Using the DCode System
Post Your Comments:
(Date:3/26/2015)... (PRWEB) March 26, 2015 PRC Clinical, ... announced today several management and executive appointments effective immediately. ... Financial Officer (CFO) and member of the Board of ... Business Operations, and Sue Dowden will assume the position ... and current Chief Operations Officer (COO), has been appointed ...
(Date:3/26/2015)... The Market Element LLC, a leading inbound marketing ... Gig LLC to provide website design, development, and creative ... alliance with Designing Gig represents a pivotal moment for ... end-to-end, digital solution for our clients, which now includes ... president of The Market Element. “For small and mid-sized ...
(Date:3/26/2015)... Massachusetts and CINCINNATI ... Shire plc (LSE: SHP, NASDAQ: SHPG ) ... a three-year, broad research collaboration for rare diseases. The ... develop novel therapies to treat rare diseases with ... commercialization capabilities with Cincinnati Children,s research expertise. As ...
(Date:3/26/2015)... March 26, 2015 PAREXEL International ... clinical research organization , announced today ... agreement to acquire all of the business ... leading provider of specialized pharmacovigilance services, based ... collection, detection, assessment, monitoring, and prevention of ...
Breaking Biology Technology:PRC Clinical Announces Key Management Appointments and Launches East Coast Operations 2PRC Clinical Announces Key Management Appointments and Launches East Coast Operations 3The Market Element LLC and Designing Gig LLC Form Strategic Alliance 2Shire and Cincinnati Children's Establish Rare Disease Research Collaboration 2Shire and Cincinnati Children's Establish Rare Disease Research Collaboration 3Shire and Cincinnati Children's Establish Rare Disease Research Collaboration 4Shire and Cincinnati Children's Establish Rare Disease Research Collaboration 5PAREXEL Announces Execution of Definitive Agreement to Acquire Quantum Solutions India, Strengthening Leadership In Pharmacovigilance Services 2PAREXEL Announces Execution of Definitive Agreement to Acquire Quantum Solutions India, Strengthening Leadership In Pharmacovigilance Services 3PAREXEL Announces Execution of Definitive Agreement to Acquire Quantum Solutions India, Strengthening Leadership In Pharmacovigilance Services 4
... - Oncolytics Biotech Inc.,("Oncolytics") (TSX:ONC, NASDAQ:ONCY) announced today ... Phase 2 clinical trial using intravenous,administration of REOLYSIN(R) ... advanced head and neck cancers. The Principal,Investigator is ... Research Center,(CTRC) at The University of Texas Health ...
... Inc. (Amex:,SGN) announced that it has filed its Form ... of trading of its common stock,on the American Stock ... trade on AMEX will be Friday, September 12, 2008.,Signalife ... for,quotation on both the Over-The-Counter Bulletin Board and the ...
... 8 On Wednesday, October,22, 2008, Wyeth (NYSE: ... quarter. The Company will hold a one-hour conference call ... 22, 2008. Interested,investors and others may listen to the ... webcast, which may be accessed by visiting our website,at ...
Cached Biology Technology:Oncolytics Biotech Inc. Starts Patient Enrolment in U.S. Phase 2 Clinical Trial Investigating REOLYSIN(R) in Combination with Paclitaxel and Carboplatin 2Oncolytics Biotech Inc. Starts Patient Enrolment in U.S. Phase 2 Clinical Trial Investigating REOLYSIN(R) in Combination with Paclitaxel and Carboplatin 3Signalife Announces Anticipated Date of Transition to OTCBB 2
(Date:3/10/2015)... , March 10, 2015  Continuing its 167-year ... Unexpected, Hammacher Schlemmer introduces The Eye Scanning Password ... before granting access to secure websites or sensitive data. ... verify travelers at international borders, the device has a ... pattern points of the iris, converting them into an ...
(Date:3/10/2015)...  NXT-ID, Inc. (NASDAQ: NXTD ... authentication company focused on the growing mobile commerce ... been named the "Number One Best Tech Gift ... Modern ( http://www.rethinkmodern.com/ ) is a web magazine focused ... that have a unique function or design.  ...
(Date:3/3/2015)... , March 3, 2015 ... provider of advanced cryogenic logistics solutions for ... immunotherapies, stem cells, cell lines, clinical research ... medicine, today announced the expansion of its ... Centers, ("Fred Hutch") Clinical Research Division ...
Breaking Biology News(10 mins):Hammacher Schlemmer Introduces The Eye Scanning Password Authenticator 2NXT-ID's Wocket Smart Wallet Named Number One Best Tech Gift for 2015 by Rethink Modern 2NXT-ID's Wocket Smart Wallet Named Number One Best Tech Gift for 2015 by Rethink Modern 3NXT-ID's Wocket Smart Wallet Named Number One Best Tech Gift for 2015 by Rethink Modern 4Cryoport, Inc. Expands Support of Fred Hutchinson Cancer Research Center's Clinical Research Division 2Cryoport, Inc. Expands Support of Fred Hutchinson Cancer Research Center's Clinical Research Division 3
... 18th century natural philosopher Jean-Baptiste Lamarck proposed that the ... cumulative effort, across generations, to reach leaves just out ... abandoned with the advent of modern genetic theories to ... medical illnesses across generations. However, there has long ...
... February 13, 2013, Cleveland: Cleveland Clinic researchers have found ... genetic screening for Lynch syndrome, the most common hereditary, ... online version of the Journal of Clinical Oncology ... cause of colon cancer in adults. The study found ...
... can affect the knowledge and behaviour of their parents, ... London surveyed the families of 160 schoolchildren in the ... about the island,s valuable wetland resources. They found ... the wetland activities were more knowledgeable about wetlands and, ...
Cached Biology News:Life experiences put their stamp on the next generation: New insights from epigenetics 2Cleveland Clinic develops clinical screening program for no.1 genetic cause of colon cancer 2Cleveland Clinic develops clinical screening program for no.1 genetic cause of colon cancer 3Environment schemes for kids influence parents' behavior 2
Goat polyclonal to T7 tag (Agarose) Immunogen: Goats were immunized with MASMTGGQQMG (T7) conjugated to KLH.After multiple immunizations in Freund's adjuvant, serum was collected....
... The features we add to WinList ... the product every day. Features like automatic ... are the result of good suggestions, thoughtful ... of the features you depend on are ...
C/EBPdelta Antibody Shipping Temperature: Cold Storage Temperature: -20C...
Rabbit polyclonal to Orexin Prepro ( Abpromise for all tested applications). entrezGeneID: 3060 SwissProtID: O43612...
Biology Products: