Navigation Links
Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

how the DCode system was used to detect a unique base pair sequence in a fragment of the16S rDNA of L. monocytogenes amplified by PCR using a general primer set and the Gene CyclerTM. The approach permitted detection of L. monocytogenes in the presence of two closely-related species, L. innocua and L. seeligeri.


Methods and Materials
THEORY OF DGGE
DGGE is a technique that separates DNA fragments of the same length but with different guanine + cytosine (G+C) base content. Separation is based on the concentration of a denaturant such as urea or formamide needed to effect separation (melting) of double-stranded helical fragments into partially-separated, single-stranded fragments. The G+C content of the fragment determines the denaturant concentration at which domains of the fragment will melt. A partially-melted fragment migrates more slowly through a polyacrylamide gel than does an undegraded, helical, doublestranded DNA molecule. The denaturation reaction can be controlled by establishing a gradient of DNA denaturants such as urea and formamide within the gel. During electrophoresis, the migrating double-stranded fragment begins to melt upon exposure of various domains of the fragment to an increasing denaturant concentration. Domains with a low %G+C will melt at a low denaturant concentration, while domains with high %G+C melt at higher denaturant concentrations further down the gel.

A 40 base pair (bp), GC-rich oligonucleotide referred to as a GC-clamp can be attached to the fragments using cloning techniques to retard migration of melted fragments through the gel to achieve better resolution of fragments with small differences in sequence variati
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13

Related biology technology :

1. DGGE Analysis Using the DCode System, Rev B
2. Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System
3. Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System
4. Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System
5. Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System
6. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
7. TTGE on the DCode System
8. Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System
9. Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms
10. Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System
11. Detection of Mutations in the CYP21 Gene Using the DCode System
Post Your Comments:
(Date:9/30/2014)... September 30, 2014 Applying a well-known optical ... at Harvard University has demonstrated the ability to ... work that holds promise for making future, flexible ... the sides of next-gen rocket ships and spacecraft ... passes through oil in water, it becomes iridescent, ...
(Date:9/30/2014)... Sept. 30, 2014 Report Details ... highest potential revenues Do you want to find ... you revenue predictions for those biological drugs from ... you find financial data, R&D trends, opportunities and ... sales forecasts to 2024 at overall world market, ...
(Date:9/30/2014)... Proove Biosciences , the commercial ... announce a new genetic test built upon research from ... Test will provide physicians with an objective way ... levels are stratified between individuals. With this new test, ... has a genetic predisposition that will affect their pain ...
(Date:9/30/2014)... Fast, semi-automatic creation of ... documents ChemAxon , a leader ... for life science research, launches ChemCurator, an application ... from documents. Markush structures are essential ... and understanding Markush structures is a difficult and ...
Breaking Biology Technology:Taking thin films to the extreme 2Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 2Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 3Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 4Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 5Proove Biosciences is Now Offering the Proove Pain Perception Genetic Test to Help Physicians Assess Patient Pain Tolerance Levels 2Proove Biosciences is Now Offering the Proove Pain Perception Genetic Test to Help Physicians Assess Patient Pain Tolerance Levels 3ChemAxon Introduces ChemCurator for Chemical Information Extraction and Markush Structure Creation 2
... ... partners to quickly and accurately forecast, plan and respond to ever-changing market demands, ManufacturingOne ... ... -- In response to a growing customer base in the high-tech manufacturing segment, ...
... ... while inviting others to join the cause for good science , ... CHAMPAIGN, IL and MELBOURNE, Australia (PRWEB) ... Web site, Academics Review , to examine claims against GM foods by Jeffrey ...
... ... guest speakers, everything sales and marketing professionals will need to pass the critical ... pipeline acceleration, inside sales, optimizing organizational structure and solutions-driven corporate/channel marketing – will ... ...
Cached Biology Technology:YASH Technologies™ Launches ManufacturingOne, a Qualified SAP Business All-in-One Partner Solution for the High-tech Manufacturing Industry 2YASH Technologies™ Launches ManufacturingOne, a Qualified SAP Business All-in-One Partner Solution for the High-tech Manufacturing Industry 3YASH Technologies™ Launches ManufacturingOne, a Qualified SAP Business All-in-One Partner Solution for the High-tech Manufacturing Industry 4Academics Launch New Web Site Holding Jeffrey Smith's Claims on GM Foods Up to Peer-Reviewed Science 2Academics Launch New Web Site Holding Jeffrey Smith's Claims on GM Foods Up to Peer-Reviewed Science 3Industry Leaders from Blue Coat, Oracle, Polycom, Siemens and Taleo Headline SiriusDecisions Sales and Marketing Summit 2010 2Industry Leaders from Blue Coat, Oracle, Polycom, Siemens and Taleo Headline SiriusDecisions Sales and Marketing Summit 2010 3Industry Leaders from Blue Coat, Oracle, Polycom, Siemens and Taleo Headline SiriusDecisions Sales and Marketing Summit 2010 4
(Date:9/30/2014)... do seedless watermelon, salmon, and strawberries all have in ... two sets of chromosomes and are diploid, these organisms ... sets of chromosomesseedless watermelon and salmon have 3 and ... While this might seem surprising, in fact most ... first discovered over a century ago, but only recently, ...
(Date:9/30/2014)... The National Institutes of Health (NIH) awarded ... to develop an electrode array system that will ... works through unprecedented resolution and scale. , LLNL,s ... support President Obama,s BRAIN (Brain Research through ... effort to revolutionize our understanding of the human ...
(Date:9/30/2014)... of environmental scientists and engineers, led by Clemson ... three-year, $5.25 million grant from the U.S. Department ... to make a direct positive impact on South ... disposal of radioactive contaminants. , "Understanding the scientific ... with environmental aspects of nuclear technologies is imperative ...
Breaking Biology News(10 mins):Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 2Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 3Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 4NIH taps lab to develop sophisticated electrode array system to monitor brain act 2NIH taps lab to develop sophisticated electrode array system to monitor brain act 3Researchers get $5.25 million to advance nuclear technologies in South Carolina 2
... Remember frozen iguanas falling from trees during Florida,s 2010 ... at the University of Miami (UM) Rosenstiel School of Marine ... numbers due to the cold conditions. "It was a ... Rosenstiel School and lead author of the study. "Centuries-old coral ...
... helicopter parents who restrict their kids, unstructured play may ... latest issue of the American Journal of Play ... group of experts to probe the near-extinction of free ... "Remarkably, over the last 50 years, opportunities for children ...
... at Oregon State University have discovered that a freshwater species ... amphibian populations around the world. This tiny zooplankton, called ... for biological control of this deadly fungus, the scientists said, ... The fungus, B. dendrobatidis , is referred to ...
Cached Biology News:New study shows that Florida's reefs cannot endure a 'cold snap' 2Lack of free play among children is causing harm, say experts in American Journal of Play 2Possible biological control discovered for pathogen devastating amphibians 2
Anti-MMP-25 (Membrane-type MMP-6, Mt6MMP), hinge region; rabbit host...
Anti-MMP-25 (Membrane-type MMP-6, Mt6MMP), hinge region; rabbit host...
Desmosomal Protein Immunogen: Full length, native protein purified from human epidermal keratins. Storage: -20 C, Avoid Freeze/Thaw Cycles...
PPIF Antibody...
Biology Products: