( 15% denaturant / 10% Acryl...)Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

> 15% denaturant / 10% Acrylamide
5% Formamide (Fischer Scientific)
1.05 M Urea (Bio-Rad)
10% Acrylamide (40% Acrylamide/Bis solution 37.5:1, Bio-Rad)
0.5x TAE (50x Tris/Acetic Acid/EDTA, Bio-Rad)
0.03125% APS Ammonium Persulfate (Bio-Rad)
0.0625% TEMED N,N,N,N-Tetramethylethylenediamine (Bio-Rad)
Dissolved in Millipore water: 6.306 g urea, add 6 ml Formamide, 25 ml 40% acrylamide, 1 ml of 50x TAE, and bring final volume to 100 ml with Millipore water. APS and TEMED are added just before use.

40% denaturant / 20% Acrylamide
Dissolve in Millipore water: 16.8168 g urea, add 16 ml Formamide, 50 ml 40% acrylamide, 1 ml of 50x TAE and bring final volume to 100 ml with Millipore water. The APS and TEMED are added just before use.

* The Polymerase Chain Reaction (PCR) Process is covered by patents owned by Hoffman-LaRoche. Use of the PCR process requires a license.

back to top
'"/>

Source:

Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13
Related biology technology :

1. DGGE Analysis Using the DCode System, Rev B
2. Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System
3. Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System
4. Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System
5. Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System
6. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
7. TTGE on the DCode System
8. Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System
9. Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms
10. Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System
11. Detection of Mutations in the CYP21 Gene Using the DCode System