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Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

> 15% denaturant / 10% Acrylamide
5% Formamide (Fischer Scientific)
1.05 M Urea (Bio-Rad)
10% Acrylamide (40% Acrylamide/Bis solution 37.5:1, Bio-Rad)
0.5x TAE (50x Tris/Acetic Acid/EDTA, Bio-Rad)
0.03125% APS Ammonium Persulfate (Bio-Rad)
0.0625% TEMED N,N,N,N-Tetramethylethylenediamine (Bio-Rad)
Dissolved in Millipore water: 6.306 g urea, add 6 ml Formamide, 25 ml 40% acrylamide, 1 ml of 50x TAE, and bring final volume to 100 ml with Millipore water. APS and TEMED are added just before use.

40% denaturant / 20% Acrylamide
Dissolve in Millipore water: 16.8168 g urea, add 16 ml Formamide, 50 ml 40% acrylamide, 1 ml of 50x TAE and bring final volume to 100 ml with Millipore water. The APS and TEMED are added just before use.


* The Polymerase Chain Reaction (PCR) Process is covered by patents owned by Hoffman-LaRoche. Use of the PCR process requires a license.


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