Navigation Links
Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

hat of the bands from the other two species may be due to a difference in %G+C of the fragments arising from the possible base differences cited above. This remains to be confirmed, however.

The general bacterial primers used in this study amplify target sequences within the 16S rRNA gene of many different bacteria. Competition for primer, enzyme and dNTPs often occurs with DNA from the different bacteria in the sample during PCR amplification. When the copy number of a gene from one type of bacterium is considerably higher than the copy number of the same gene from another type of bacterium (i.e., an abundance of cells of one species over cells of another), the gene with the greater copy number will be amplified preferentially over the gene present in lower copy number. An experiment was conducted to determine the minimal ratio of L. monocytogenes DNA to L. innocua and L. seeligeri DNA that still enabled detection of the unique band from L. monocytogenes in DGGE gels after fragment amplification by PCR using PfuTurbo polymerase and primer pair 1055F and 1406R with GC-clamp. The results indicate that L. monocytogenes-specific band could still be detected in the presence of 23 times its weight of DNA contributed by the other two species. When the difference in relative amounts were above this, the L. monocytogenes-specific band was undetectable.

In summary, DGGE using the DCode system facilitates detection of variations in 16S rDNA sequence that result in different G+C content among closely-related bacterial species as well as among multiple alleles of the same gene in a single species. Separation based on subtle differences in G+C content by DGGE into discrete visible bands facilitates subsequent isolatio
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13

Related biology technology :

1. DGGE Analysis Using the DCode System, Rev B
2. Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System
3. Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System
4. Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System
5. Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System
6. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
7. TTGE on the DCode System
8. Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System
9. Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms
10. Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System
11. Detection of Mutations in the CYP21 Gene Using the DCode System
Post Your Comments:
(Date:5/21/2013)... 2013 First patients dosed with ... bromide designed using Prosonix, particle engineering platform ... of inhaled Respiratory Medicines by Design , has ... will assess its effect on lung function and the ... moderate to severe chronic obstructive pulmonary disease (COPD). The ...
(Date:5/21/2013)... Bellingham, WA (PRWEB) May 21, 2013 ... teacher trainings, and funds for lab equipment, 25 organizations ... relevance and importance of optics and photonics in daily ... Grants . , The organizations, located in 10 countries, ... grants to be awarded by SPIE, the international ...
(Date:5/21/2013)... Savoury Systems International (SSI), a leader in ... Southeastern USA Sales Broker. Braddy’s new role will allow ... Southeast. , In 2004 Braddy founded Southeastern ... food processors and industrial manufacturers. Braddy has worked ... and has over 25 years of sales management experience. ...
(Date:5/21/2013)... As the R&D sector becomes ... the industry to deliver better products, faster. Collaborations ... companies often means turning big ideas into reality, ... collaboration with Sensidose AB and Recipharm, is one ... to market sooner. The partnership resulted in the ...
Breaking Biology Technology:Prosonix Initiates Phase 2 Clinical Study with PSX1002 in Patients with Moderate to Severe Chronic Obstructive Pulmonary Disease (COPD) 2Prosonix Initiates Phase 2 Clinical Study with PSX1002 in Patients with Moderate to Severe Chronic Obstructive Pulmonary Disease (COPD) 3Prosonix Initiates Phase 2 Clinical Study with PSX1002 in Patients with Moderate to Severe Chronic Obstructive Pulmonary Disease (COPD) 4Learning About Light: Education Outreach Projects Funded in 10 Countries Through First Round of SPIE Grants 2Learning About Light: Education Outreach Projects Funded in 10 Countries Through First Round of SPIE Grants 3The Development of an Innovative Microtablet Dosage Form, a Success Story: Live Webinar Hosted by Xtalks 2
... BURLINGAME, Calif., March 11, 2011 Apexigen, Inc., an ... antibody therapeutics, announced today that its partner, Simcere Pharmaceutical ... company in China, filed an Investigational New Drug (IND) ... of China to evaluate APX003 (also known as BD0801) ...
... consumer products continue to grow at a consistent ... Emerging Nanotechnologies (PEN) over 1,300 manufacturer-identified, nanotechnology-enabled products ... The most recent update to the group,s five-year-old ... particles in everything from conventional products like non-stick ...
... Dyadic International, Inc. (OTC Pink: DYAI), a global biotechnology ... December 31, 2010. The financial information contained in this ... financial statements, footnotes and independent auditors, report which have ... www.otcmarkets.com and on Dyadic,s website at www.dyadic.com ...
Cached Biology Technology:Apexigen Announces the Filing by its Partner, Simcere Pharmaceutical Group, of First IND 2Dyadic International Reports 2010 Fiscal Year Financial Results 2Dyadic International Reports 2010 Fiscal Year Financial Results 3Dyadic International Reports 2010 Fiscal Year Financial Results 4Dyadic International Reports 2010 Fiscal Year Financial Results 5Dyadic International Reports 2010 Fiscal Year Financial Results 6
(Date:5/21/2013)... new method for delivering molecules into single, targeted ... The technique could find applications in drug delivery, ... electroporation a technique used to deliver molecules ... membrane that are caused by exposing them to ... of cell transfection. (Cell transfection is the introduction ...
(Date:5/21/2013)... in pharmacies and health stores, phosphatidylserine is a ... soy. Proven to improve cognition and slow memory ... experiencing memory impairment. Now a team headed by ... Tel Aviv University,s Department of Human Molecular ... improves the functioning of genes involved in degenerative ...
(Date:5/21/2013)... Society (ACS), the world,s largest scientific society, invites ... & Exposition, September 8-12, 2013, in Indianapolis, Ind. ... Center and at area hotels. , With more ... science,s horizons from astronomy to zoology ― ... journalists a rich assortment of spot news and ...
Breaking Biology News(10 mins):Single-cell transfection tool enables added control for biological studies 2Common food supplement fights degenerative brain disorders 2
... 2010 In any battle, sizing up one,s opponent ... bacterial infection, that means assessing every nook and cranny ... attack. At the Center for Biological Research of ... maneuvers they hope will take out bacteria at their ...
... as the Most Innovative Company of the Year in Europe ... LIVINGSTON, ... ), developer of advanced upper-limb bionic technologies and clinical solutions, today ... Company of the Year in Europe in the 2010 ...
... Elsevier, a world-leading publisher of scientific, ... announced that Collexis, an Elsevier business and leading ... has launched its community module for their Expert ... Profiling connects experts across the Collexis community from ...
Cached Biology News:In the ring: Researchers fighting bacterial infections zero in on microorganism's soft spots 2In the ring: Researchers fighting bacterial infections zero in on microorganism's soft spots 3Touch Bionics announced as a winner in the Seventh Annual International Business Awards 2Touch Bionics announced as a winner in the Seventh Annual International Business Awards 3Collexis launches community feature for academic customers 2
... produces output at 470 nm; includes interface cable ... or installed into rack or desktop boxes ... is an LED that produces either pulsed or ... region -- for fluorescence measurements. The source is ...
Sterile, Cell culture tested...
With sodium bicarbonate. Sterile, Cell culture tested...
... Cost effective - Utilizes hands-off homogenization through a ... No grinding - Extract DNA without hand grinding; ... samples , Robust tubes - Includes ... without tube breakage , Flexible format ...
Biology Products: