Navigation Links
Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

Nikolaj Schmolker, Fintan van Ommen Kloeke and Gill Geesey, Department of Microbiology and Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717-3980


Abstract
Amplification of a region of the 16S rRNA gene of L. monocytogenes, Listeria innocua, and Listeria seeligeri by the polymerase chain reaction (PCR)* using primers that anneal to conserved sequences, yielded multiple copies of gene fragments that, when evaluated by density gradient gel electrophoresis (DGGE), produced banding patterns that distinguished L. monocytogenes from the other two species. One set of primers yielded fragments from the 16S rRNA gene(s) of L. monocytogenes that gave rise to two distinct DGGE bands: one of which co-migrated with the single bands produced from the other two species, and another not produced by the other two species, which migrated to a location higher on the gel than the other band.


Introduction
Detection and quantification of food-borne pathogenic bacteria such as Listeria monocytogenes have historically depended on culture techniques that require an incubation period of several days. Use of procedures that involve culture and incubation for positive identification of causative agents of disease delays implementation of preventive action to control the spread of disease. New molecular techniques that provide positive identification of the causative agent of disease without the need to culture the suspect microorganism(s) facilitate a more rapid response to the problem. Using the polymerase chain reaction (PCR), positive identification of L. monocytogenes can be established within 1248 h of sample collection.1-3

Numero
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13

Related biology technology :

1. DGGE Analysis Using the DCode System, Rev B
2. Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System
3. Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System
4. Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System
5. Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System
6. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
7. TTGE on the DCode System
8. Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System
9. Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms
10. Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System
11. Detection of Mutations in the CYP21 Gene Using the DCode System
Post Your Comments:
(Date:8/27/2014)... YORK , Aug. 27, 2014 ... is available in its catalogue: Global ... http://www.reportlinker.com/p02316662/Global-Chelating-Agents-Market-2014-2018.html About Chelating Agent A ... form covalent bonds with metal ions, thereby forming ... that some metal-ions have on chemical processes, formulations, ...
(Date:8/27/2014)... 27, 2014 Guggenheim Securities, the investment banking ... the hiring of veteran equity analyst Charles "Tony" Butler ... Butler will focus on the biotech/biopharma sector. ... of experience and a broad network of relationships in ... Director and Head of Equities at Guggenheim Securities. "His ...
(Date:8/27/2014)... -- ARCH Venture Partners, one of the largest U.S. ... seed and early-stage advanced technology companies, today announced ... than $400 million in subscriptions. ARCH Venture Fund ... more than $150 million. "The enthusiasm ... of our approach to finding and funding new ...
(Date:8/27/2014)... (PRWEB) August 27, 2014 According to ... continues to reshape the look and feel of markets ... demand for higher-quality ingredients will push for more branded, ... market have experienced strong market growth in the last ... in their food and cosmetics to fit a more ...
Breaking Biology Technology:Global Chelating Agents Market 2014-2018 2Global Chelating Agents Market 2014-2018 3Global Chelating Agents Market 2014-2018 4Guggenheim Securities Hires Analyst Charles "Tony" Butler to Expand Healthcare Coverage 2Latest ARCH Venture Partners Fund Closes Above $400 Million 2Latest ARCH Venture Partners Fund Closes Above $400 Million 3Daring to be Different Raw Materials & Ingredient Supply Shortage Fuels Vital Force™ Technology New Ingredient Enhancements 2Daring to be Different Raw Materials & Ingredient Supply Shortage Fuels Vital Force™ Technology New Ingredient Enhancements 3
... BUFFALO, N.Y. -- A novel device, developed by ... simply and conveniently traps, detects and manipulates the ... obstacles that have prevented progress toward spintronics and ... Physical Review Letters, the research paper brings closer ...
... 28 PPD, Inc. (Nasdaq:,PPDI) will release its third ... market closes. The earnings release will be available,on the ... PR Newswire. On Wednesday, October 24, at 9 ... audio webcast to discuss financial results and the,overall business ...
... LAVAL, QC, Sept. 28 /PRNewswire-FirstCall/ - Neptune Technologies ... report its,financial results for the fiscal year ended ... improved its productivity and operational,profitability while continuing its ... Asian markets, resulting in,sustainable sales growth. Financial ...
Cached Biology Technology:Quantum device traps, detects and manipulates the spin of single electrons 2PPD Announces Third Quarter 2007 Earnings Release, Webcast and Conference Call 2Neptune Reports Year End Financial Results - Neptune Records 18% Sales Increase and 43% EBITDA Increase in Year End Results 2Neptune Reports Year End Financial Results - Neptune Records 18% Sales Increase and 43% EBITDA Increase in Year End Results 3
(Date:8/27/2014)... LAKE CITY, Aug. 27, 2014 University of Utah ... his native Turkey, has won that nation,s highest science ... of Science. , Sekercioglu is among five researchers picked ... and Technical Research Council of Turkey. Three researchers won ... won the Special Award, which is equivalent to the ...
(Date:8/27/2014)... the greatest risk to Europe based upon a proxy ... researchers using a ,big data, approach to scientific research. ... and Global Health ranked the top 100 pathogens affecting ... a system which, they believe, will help governments across ... of infectious diseases, including as a result of climate ...
(Date:8/27/2014)... 27, 2014 /PRNewswire-iReach/ -- The IEEE Engineering in Medicine ... Annual International Conference (EMBC,14), this year being held ... Chicago, Illinois , from Tuesday, August 26 ... students, and industry professionals in science, medicine, and engineering ... http://photos.prnewswire.com/prnh/20140827/140333 This year,s conference theme ...
Breaking Biology News(10 mins):University of Utah biologist wins Turkey's top science prize 2Big data approach identifies Europe's most dangerous human and domestic animal pathogens 2Global Leaders In Science, Medicine, And Engineering Gather For The 36th Annual International Conference Of The IEEE Engineering In Medicine And Biology Society, August 26-30 In Chicago 2Global Leaders In Science, Medicine, And Engineering Gather For The 36th Annual International Conference Of The IEEE Engineering In Medicine And Biology Society, August 26-30 In Chicago 3
... the year,s end-of-life refrigerators, TVs, mobile phones, computers, monitors, ... cord worldwide could fill a line of 40-ton trucks ... Equator. That forecast, based on data compiled by ... of UN organizations, industry, governments, non-government and science organizations ...
... many of the harmful physiological effects of short-term overeating and ... The Journal of Physiology , which is well timed with ... even a few days of energy surplus where you ... impacts. This new study shows that a daily bout of ...
... organisms evolved from earlier life forms. At the ... help complete chemical processessuch as converting food into energyin ... University of Iowa researcher and his colleagues describe the ... as it occurred from bacteria to humans. Their paper, ...
Cached Biology News:World e-waste map reveals national volumes, international flows 2World e-waste map reveals national volumes, international flows 3World e-waste map reveals national volumes, international flows 4World e-waste map reveals national volumes, international flows 5Exercise counters the physiological effects of Christmas excess 2UI researcher studies evolution on the molecular level 2
... Validation Service enables early selection of ... by providing quantitative data for sensitivity, ... each antibody. Furthermore quantitative antibody fingerprints ... activity of your antibodies., The full-service ...
... fluorescent microscopy-based imaging system that accurately counts ... calculates cell viability, in a single test. ... variability associated with the subjective interpretation of ... EasyCount ViaSure Kit, up to six tests ...
... new generation of microarrays to detect alternative ... to rapidly elucidate alternatively spliced events in ... drug discovery. The service combines ExonHits proprietary ... customized analysis with Agilents robust microarray platform. ...
... The new Adept CE 4100 ... provides superb flow precision, with software drive ... rates from 0.001-10mL/min are selectable in 0.001mL ... inserts, are inert and bio-compatible., Pumps may ...
Biology Products: