Navigation Links
Use of ZipTip Pipette Tips for Microscale Affinity Mass Spectrometry

f the transferrin with the C18 resin or with the plastic tip, a simple control experiment was performed.

The ZipTip pipette tip was incubated with or without anti-transferrin prior to blocking with BSA. In addition, 0.05% Tween 20 was either added or not added to the sample. Thus, one tip was an anti-transferrin tip and the other a BSA ZipTip pipette tip. In panel B of Figure 3, it can be seen in the bottom spectrum (organic elution) that little transferrin was bound in the absence of anti-transferrin IgG. However, when anti-transferrin was present, transferrin was strongly detected after acid elution (panel A, acid elution). In the absence of Tween 20, stronger non-specific binding was observed (data not shown).

Conclusion

When coupled to MALDI-TOF mass spectrometry, the ZipTip pipette tip makes an excellent platform for microscale affinity purification and identification.


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Brinkmann/Eppendorf SOP Standard Operating Procedure for Pipettes
2. Highly Active and Removable AffinityTagged Lambda Phosphatase
3. Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit
4. Affinity Depletion Cartridges for Removal of Human Serum Albumin and Immunoglobulins from Human Serum
5. Identification of Low-Level Oxidation Products in Calmodulin Using NanoSpray Ion Trap Mass Spectrometry
6. Determination of N-nitrosamines in Baby Bottle Rubber Teats by Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry
7. Small-Molecule Quantitation Using Automated Single-Shot Averaging MALDI-TOF Mass Spectrometry
8. Achieving Reliable Protein Quantitation Results Using ICAT Reagents, MALDITOF Mass Spectrometry, and Advanced Data Interpretation Tool
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Use ZipTip Pipette Tips for Microscale Affinity Mass Spectrometry

(Date:11/24/2014)... Nov. 24, 2014  Last week, Representatives ... (D-NC) took a bold bipartisan step on ... the OPEN Act. Original co-sponsors of the legislation ... Act will incentivize drug makers and innovators to ... and pediatric cancers, which opens the door to ...
(Date:11/24/2014)... 2014 Continental Clinical Solutions, LLC ... clinical trials at its mid-Atlantic late-phase research facility, ... created with the idea of closing patient disparities ... in medical advances," said Continental's CEO, Saleh ... the immortal cell line of Henrietta Lacks, dubious ...
(Date:11/23/2014)... Apiscent Labs, a privately held ... ingredients to the global pharmaceutical and flavor & ... website, Apiscent.com. , The Apiscent.com homepage welcomes visitors ... easy navigation, rotating images and content focused on ... , The company’s work in Pharmaceutical ...
(Date:11/22/2014)... MD (PRWEB) November 21, 2014 ... management solutions provider, has released Limfinity® version 5.1.4. ... installation projects of Limfinity® than ever in RURO’s ... configuration and minority framework enhancement. Limfinity® version 5.1.4 ... far above and beyond bug fixes! , Limfinity® ...
Breaking Biology Technology:OPEN Act Introduced to Repurpose Drugs for Rare Diseases 2At Continental Clinical Solutions, CEO Saleh Stevens Focuses on Diversity in Clinical Trial Recruitment at Maryland Site 2At Continental Clinical Solutions, CEO Saleh Stevens Focuses on Diversity in Clinical Trial Recruitment at Maryland Site 3RURO Announces Limfinity® version 5.1.4 2
... even the resources of the world,s largest industrialized nations, scientists ... to find solutions. , Mark Shannon of the University of ... water resource research to address this crisis in a review ... "As dire as the growing problems are with a lack ...
... Blacksburg, Va. -- The ubiquity of mineral nanoparticles in ... intriguing properties provide Earth scientists with another dimension in ... team of scientists from seven universities in a review ... Nanominerals, Mineral Nanoparticles, and Earth Chemistry. , The way ...
... March 20 Scientists at the,Bio-Communications Research ... for,the Improvement of Human Functioning International (CIHFI), ... about its recent stem cell,discovery. The scientific ... stem cell population" received BioMed Central,s,Research Article ...
Cached Biology Technology:Countering an approaching water crisis 2Nanoscience will change the way we think about the world 2Nanoscience will change the way we think about the world 3Nanoscience will change the way we think about the world 4Nanoscience will change the way we think about the world 5Medical Research Award Goes to Adult Stem Cell Scientists at a Kansas Not-for-Profit Organization 2
(Date:11/11/2014)... -- Forensicon, Inc., a Chicago -based digital ... promotion of Yaniv Schiff from Senior Computer ... new role as Director, he will lead company efforts ... and provide leadership within the company,s digital forensics practice ... Schiff joined Forensicon in 2006 and ...
(Date:11/7/2014)... -- In conjunction with the Glendale and ... of the Verdugos has announced the " GLENDALE ... initiative to keep the Verdugo Regional Crime Lab operational after ... of existence, the Lab has processed more than 400 DNA ... "Because our number one priority is the wellbeing ...
(Date:11/6/2014)... cancer deaths occur because of metastasis, yet progress ... been slow. , "It,s been particularly challenging to ... Gujral, research fellow in systems biology at Harvard ... detected until after they,ve already metastasized." , Gujral ... that should help researchers better understand how metastasis ...
Breaking Biology News(10 mins):Yaniv Schiff Promoted to Director of Digital Forensics at Forensicon, Inc. 2Community Foundation Of The Verdugos Unveils Campaign To Fund Glendale-Burbank DNA Justice Project 2Migration negation 2Migration negation 3
... of cell and developmental biology, has been awarded one ... Awards, one of the NIH,s most prestigious grants. ... address a significant medical science challenge: identifying the full ... and disease processes such as cancer. Major, a ...
... professors have been named 2010 fellows of the American ... and Interdisciplinary Studies (CBIS) and the Howard P. Isermann ... and Professor Emeritus of Chemistry Leonard Interrante were both ... and contributions to the science, the profession, and service ...
... parts of the world have brought into sharp focus new ... of rivers during periods of high flow. Using an ... how the flow of river water affects the movement of ... changes in the bed of an artificial water channel to ...
Cached Biology News:UNC scientist receives NIH director's New Innovator Award 2Rensselaer professors Dordick and Interrante named ACS Fellows 2What lies beneath: Study examines sediment movement during floods in rivers 2What lies beneath: Study examines sediment movement during floods in rivers 3
...
... Conjugation Kits are based on well-established ... are consistent and reproducible. This kit ... your protein or antibody, including ready-to-use ... Activated HRP is supplied ready-to-use ...
Convenient 10X buffer concentrate used to maximize binding to the solid phase in microwell ELISA. Formulated to provide optimal pH and ionic strength for binding of antibodies and some antigens. Co...
... PCR detection system holds 48 x 0.2 ml ... 48 LEDs independently excites fluorescence (470-500 nm) for ... detects emitted light (523-543 nm and 540-700 nm). ... cycler, whose Peltier heat pumps provide superb thermal ...
Biology Products: