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Use of ZipTip Pipette Tips for Microscale Affinity Mass Spectrometry

f the transferrin with the C18 resin or with the plastic tip, a simple control experiment was performed.

The ZipTip pipette tip was incubated with or without anti-transferrin prior to blocking with BSA. In addition, 0.05% Tween 20 was either added or not added to the sample. Thus, one tip was an anti-transferrin tip and the other a BSA ZipTip pipette tip. In panel B of Figure 3, it can be seen in the bottom spectrum (organic elution) that little transferrin was bound in the absence of anti-transferrin IgG. However, when anti-transferrin was present, transferrin was strongly detected after acid elution (panel A, acid elution). In the absence of Tween 20, stronger non-specific binding was observed (data not shown).

Conclusion

When coupled to MALDI-TOF mass spectrometry, the ZipTip pipette tip makes an excellent platform for microscale affinity purification and identification.


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