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Use of Transcriptor Reverse Transcriptase,,,in Microarray Analysis

general, the data above suggest that Transcriptor Reverse Transcriptase produces a labelled target with results similar to those of the updated RNase HM- MuLV reverse transcriptase. But Transcriptor Reverse Transcriptase appears to produce a more consistent labelled target, as the four replicates generally correlate better, with noticeably fewer outlier spots. The known enzymatic properties of Transcriptor Reverse Transcriptase (high processivity and RNase H activity) should produce a labelled target that closely reflects the experimental input mRNA. This will be critical for using the latest generation of oligonucleotide microarrays, which include probes designed to the 3′ end of a transcript to facilitate the analysis of mRNA splice variants, multiple poly-A+ addition sites, etc.




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