Navigation Links
Use of Transcriptor Reverse Transcriptase,,,in Microarray Analysis

RNA was used in all experiments, it was possible to test an enzymes ability to generate reproducible results; microarrays using Transcriptor Reverse Transcriptase and product Y were replicated four times each. Ratio (Cy5/Cy3) data from each of the four replicates plotted against the mean show similar results, where the Transcriptor Reverse Transcriptase replicates show generally better correlation to the mean than product Y (Figure 3). This appears to be due to fewer outlier spots that depart substantially from the trend lines in the four Transcriptor Reverse Transcriptase replicates.



The replicate data were processed in GeneSpring to detect particular spots that are consistently biased towards greater signal in either Transcriptor Reverse Transcriptase or product Y. Inspection of a few cDNA probes showed that the greatest signal bias towards Transcriptor Reverse Transcriptase appear to be in those probes that are substantially truncated, and those with a greater signal bias towards product Y are truncated much closer to the 5′ end. Although this anecdotal observation could be interpreted in several ways, it is at least consistent with the notion that the high processivity of Transcriptor Reverse Transcriptase may produce a better labelled target and more robust data in cases where the spot/probe cDNA is substantially truncated.

Conclusion

The histogram of signal intensity distributions show that Transcriptor Reverse Transcriptase can be used for high efficient target labelling. Since many investigators eliminate data with signal intensities below a particular threshold (e.g.,1,0002,000), an increased specific activity of the labelled target may be critical for analysing genes with low expression.

In
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Transcriptor Reverse Transcriptase
2. Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase
3. Properties of Reverse Transcriptases
4. Reverse transcriptases suitable for one-step RT-PCRb
5. Reverse transcriptases suitable for two-step RT-PCRd
6. C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR
7. Recombinant HIV Reverse Transcriptase
8. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
9. New Microarray Labeling Kit for Preparing cDNA Probes
10. Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes
11. An Optimal DNA Microarray Substrate for the Identification of Fetal and Somatic Liver Stem Cells of the Rat
Post Your Comments:
(Date:12/19/2014)... DUBLIN , Dec. 19, 2014 Research ... announced the addition of the "Technology Innovations ... their offering. http://photos.prnewswire.com/prnh/20130307/600769 ... Innovations in Smart Fabrics" offers a detailed assessment ... Key Findings: 1. ...
(Date:12/19/2014)... ME (PRWEB) December 18, 2014 Ed ... director of the Center for Excellence in the Neurosciences ... New England, has been selected as a member of ... is the first neuroscientist from Maine to receive this ... nonprofit organization supported entirely by the Dana Foundation. ...
(Date:12/19/2014)... The empty capsules market is experiencing ... and technological innovations in the empty capsules market. ... the growth of the empty capsules market. , ... Scope of Report , The report will enrich ... firms to gauge the pulse of the market ...
(Date:12/19/2014)... 18, 2014   ISN has released its ... Indicators publication series. With data aggregated from ... in 2013 within its online contractor management platform, ISNetworld, ... performance indicator (KPI) statistics. The ... publication is available in two industry specific volumes: ...
Breaking Biology Technology:Technology Innovations in Smart Fabrics (Technical Insights) 2UNE's Ed Bilsky Named Member of Dana Alliance for Brain Initiatives 2UNE's Ed Bilsky Named Member of Dana Alliance for Brain Initiatives 3Empty Capsules Market Hit to Reach $1,820 Million by 2019 - New Research Report by MarketsandMarkets 2Empty Capsules Market Hit to Reach $1,820 Million by 2019 - New Research Report by MarketsandMarkets 3Empty Capsules Market Hit to Reach $1,820 Million by 2019 - New Research Report by MarketsandMarkets 4ISN Releases Third Edition of US Contractor Health & Safety Performance Indicators Publication Series 2ISN Releases Third Edition of US Contractor Health & Safety Performance Indicators Publication Series 3
... have another chance to strengthen ties with Japan at ... Center. , ,Sponsored by the Japan External Trade ... an inside look into how Wisconsin biotech companies are ... traveled to Japan will talk about the difficulties and ...
... are available 24 hours a day now that a new ... , ,The Web site MusicMadeInMadison.com plays more than ... the Profits, Daedalus Burn, Infinite Helix and They Drive By ... site's goal is to help both the circulation of the ...
... and Human Services Secretary Mike Leavitt announced yesterday that ... will be formed to make recommendations for a nationwide ... , ,This news makes the Digital Healthcare Conference ... 9 at the Fluno Center in Madison, even more ...
Cached Biology Technology:Wisconsin biotechs plan Japan trade mission 2Preview: Electronic health records, tech choices explored at Digital Healthcare Conference 2Preview: Electronic health records, tech choices explored at Digital Healthcare Conference 3
(Date:12/17/2014)... Dec. 16, 2014 Research and Markets ... of the "Global Chemical Sensor Market 2015-2019" ... http://photos.prnewswire.com/prnh/20130307/600769 One major ... demand for medical sensors in biomedical applications. Chemical ... quick and correct diagnosis during surgical procedures. The ...
(Date:12/15/2014)... Dec. 12, 2014 Research and Markets ... of the "Global Facial Recognition Market 2015-2019" ... http://photos.prnewswire.com/prnh/20130307/600769 Facial recognition ... individuals. Facial recognition system measures the overall facial ... edges, mouth, and the distance between eyes. Facial ...
(Date:12/11/2014)... 2014 Research and Markets ... ( http://www.researchandmarkets.com/research/9ql3kr/biometrics_market ) has announced the addition of ... report to their offering. One ... adoption of multimodal biometric systems. Multimodal biometric systems ... for verification and identification purposes. This helps to ...
Breaking Biology News(10 mins):Global Chemical Sensor Market 2015-2019: Key Vendors are Abbott Laboratories, Bayer, Hoffmann La-Roche, Johnson & Johnson, NGK Spark Plugs and Robert Bosch 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 3Biometrics Market in India 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2
... are spiritual, according to new research from Rice University. Though ... that spirituality is a separate idea one that more ... The research will be published in the June ... in-depth interviews with 275 natural and social scientists at elite ...
... human fungal infections caused by certain strains of Aspergillus ... treatments at an alarming rate, say scientists. University of ... a new test that can not only better diagnose ... resistance to azoles the class of drugs used to ...
... at Sam Houston State University and the University of Texas ... justice system in Houston to establish protocols to determine when ... , "This is a problem-solving project that seeks to determine ... William Wells, who is leading the research project at Sam ...
Cached Biology News:More than 20 percent of atheist scientists are spiritual 2Drug-resistance fears for deadly fungal disease 2Research to target untested rape kits 2Research to target untested rape kits 3
Request Info...
...
...
... purified Formulation: 50mM Tris-HCl, pH ... 0.2mM PMSF, 1mM benzamidine, 0.1% ... PP2A1 (Protein Phosphatase 2A1) partially ... sulfate precipitation, QSepharose,amino-hexyl agarose, Superdex ...
Biology Products: