Navigation Links
Use of Transcriptor Reverse Transcriptase,,,in Microarray Analysis

ource is used throughout and each target labelling represents an independent reverse-transcriptase and dyecoupling reaction. Data from each microarray are derived from two independently labelled targets simultaneously hybridised to a single microarray.



Although the relative specific activities of a hybridised labelled target can be estimated by total mean fluorescence over an entire microarray signal set, the distribution of signal intensities on a per-spot (probe) basis is more informative. Figure 2 illustrates a histogram of three representative sets of 9,737 raw (nonnormalised) fluorescent signals from the Cy5 channel. Compared with Transcriptor Reverse Transcriptase there is a much greater abundance of signals in the 0 5,000 class and relative paucity of signals in the higher fluorescence classes for product X. This signal distribution indicates that Transcriptor Reverse Transcriptase labels cDNA more efficiently than product X. In contrast, Transcriptor Reverse Transcriptase and product Y show similar signal distributions where more than half of the signals were in classes greater than 5,000. Members of the highest signal class are signals at saturation, some of which are T. gondii gene transcripts known to be in very high abundance. The high number of signals in this class for Transcriptor Reverse Transcriptase indicates a relatively strong labelling reaction compared with the low signal numbers for product X. Since the optimal laser power and PMT levels were similar for all three microarray scans, image acquisition can not account for the difference in results.



Consistency of target labelling is critical for microarray analysis of gene expression to detect real transcriptional differences between experimental treatments. Since the same input
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Transcriptor Reverse Transcriptase
2. Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase
3. Properties of Reverse Transcriptases
4. Reverse transcriptases suitable for one-step RT-PCRb
5. Reverse transcriptases suitable for two-step RT-PCRd
6. C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR
7. Recombinant HIV Reverse Transcriptase
8. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
9. New Microarray Labeling Kit for Preparing cDNA Probes
10. Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes
11. An Optimal DNA Microarray Substrate for the Identification of Fetal and Somatic Liver Stem Cells of the Rat
Post Your Comments:
(Date:11/26/2014)... HOUSTON and PHILADELPHIA , ... the most demanding and unforgiving environments. Human exploration of ... cognitive performance to ensure mission safety and success, and ... cognitive performance for mission success, little is known about ... aspects of cognition are primarily affected. Now, ...
(Date:11/26/2014)... The ETC (Emerging Technology Centers) http://www.etcbaltimore.com , ... that applications for AccelerateBaltimore™ (AB) 2015 program will ... a big idea?,” asked ETC’s President Deb Tillett. ... AccelerateBaltimore helps you and your big idea go ... Interested game changers with “big ideas” should ...
(Date:11/26/2014)... November 25, 2014 SonaCare Medical, ... Ultrasound (HIFU) devices, recently participated in the American ... Mass 360° .” Key opinion leaders in urology ... related to small renal masses while attendees had ... ablative technologies in hands-on labs. Attendees at the ...
(Date:11/26/2014)... 2014 The Pittcon marketing department is ... and exposition mobile app, Pittcon 2015 ... the App Store for iOS and Android devices and ... before, during and after the event. It acts as ... companies, technical sessions, Conferee Networking sessions and short courses. ...
Breaking Biology Technology:Penn Medicine Team Develops Cognitive Test Battery to Assess the Impact of Long Duration Spaceflights on Astronauts' Brain Function 2Penn Medicine Team Develops Cognitive Test Battery to Assess the Impact of Long Duration Spaceflights on Astronauts' Brain Function 3Penn Medicine Team Develops Cognitive Test Battery to Assess the Impact of Long Duration Spaceflights on Astronauts' Brain Function 4AccelerateBaltimore Applications to Close in 6 Days 2AccelerateBaltimore Applications to Close in 6 Days 3SonaCare Medical Supports American Urology Association’s Small Renal Mass 360° Summit 2SonaCare Medical Supports American Urology Association’s Small Renal Mass 360° Summit 3Pittcon Releases 2015 Mobile App 2
... (Nasdaq: MNKD ), focused on discovering, developing and,commercializing ... present at the UBS Global Life Sciences Conference on ... Hyatt Hotel in New York, NY. Interested parties ... of the,presentation in the Investor Relations section of the ...
... Sept. 18 InterMune, Inc.,(Nasdaq: ITMN ) today ... other conditions, 3,000,000 shares of its common stock in ... underwriters,a 30-day option to purchase up to an additional ... All of the shares in the offering,will be sold ...
... Sept. 18 /PRNewswire-FirstCall/-- Biodel Inc. (Nasdaq:,BIOD) today ... program,at the 43rd Scientific Sessions of the ... VIAject(TM) Phase II meal study data ... entitled, "Pharmacokinetics and pharmacodynamics of,insulin VIAject(TM), insulin ...
Cached Biology Technology:InterMune Announces Proposed Public Offering of Common Stock 2Biodel Inc. Announces VIAject(TM) Phase II Meal Study Data at the 2007 European Association for the Study of Diabetes Meeting 2Biodel Inc. Announces VIAject(TM) Phase II Meal Study Data at the 2007 European Association for the Study of Diabetes Meeting 3
(Date:11/6/2014)... occur because of metastasis, yet progress in preventing ... , "It,s been particularly challenging to design drugs ... fellow in systems biology at Harvard Medical School. ... after they,ve already metastasized." , Gujral and colleagues ... help researchers better understand how metastasis begins. Their ...
(Date:11/5/2014)... his career to understanding the Earth,s climate and ... As deputy director and regional climatologist for the ... Institute, Redmond has more than three decades of ... climate data to the general public. , At ... on December 15-19, 2014 the American Geophysical Union ...
(Date:11/4/2014)... – Temple University School of Medicine announced today ... an initiative funded by the Bill & Melinda ... of Obstetrics, Gynecology and Reproductive Sciences; and Vice ... University School of Medicine will pursue an innovative ... Blood Biomarkers to Assess Fetal Neurodevelopment.", Grand Challenges ...
Breaking Biology News(10 mins):Migration negation 2Migration negation 3Nevada climatologist to be honored at AGU Fall Meeting for lifetime of public outreach 2Temple University School of Medicine receives Grand Challenges Explorations grant 2
... Renal diseases shall be diagnosed earlier and treated more ... nine European countries*, coordinated by the Max Delbrck Center ... for the past four-and-a half years to create a ... findings on the development and diseases of the kidney. ...
... Stress may indeed be a direct contributor to childhood ... study finding that increased levels of stress in adolescents ... overweight or obese. The study of 1,011 adolescents ... living in three cities -- Boston, Chicago and San ...
... - A new article published in Elsevier,s journal Bioscience ... our lifetime can affect our children. Dr. Halabe Bucay ... generates when we are in different moods could affect ,germ ... next generation. Such natural chemicals could affect the way that ...
Cached Biology News:3-D kidney atlas created for researchers and physicians 2Study finds link between individual stress and adolescent obesity 2Study finds link between individual stress and adolescent obesity 3
Request Info...
... 30 L samples, featuring 24-position turntable. Provides up to 30 minutes of walkaway time. ... analysis and record keeping. RS-232 port. ... ... ...
... mL - 25 mL pipets ,Angled acrylic pipet ... standard square or round pipet cans. Constructed of 6 ... 76 mm W x 76 mm H x 289 ... Overall dimensions: 89 mm W x 406 mm H ...
...
Biology Products: