Navigation Links
Use of On-line Size Exclusion Chromatography (SEC) for Sample Clean-up in LC/MS Analyses

Chromatography and Mass Spectrometry Application Report

Tania A. Sasaki and Paul Shieh, Thermo Electron Corporation, San Jose, CA

Introduction
Use of LC/MSn has experienced a dramatic increase in applications throughout the drug discovery and development process, from high-throughput screening to ADME/Tox studies. The Finnigan LCQ series ion trap mass spectrometers meet the demands for rapid screening of large numbers of drug candidates and their metabolites because of their sensitivity, high selectivity of MSn, ease of use, and ability to tolerate complex biological matrices. Often, compounds analyzed for drug discovery are present in biological matrices, such as plasma and serum. The task of monitoring target drug compounds and/or metabolites in these matrices can present considerable challenges when trying to achieve optimum separation, identification, and detection. It is often necessary to incorporate a time-consuming sample clean-up step into the analytical method. However, because of the growing emphasis on rapid method development and analysis in drug discovery and development, it is desirable to minimize the number of steps and overall time required in the analytical method without compromising data quality.

Goal
This report demonstrates the use of SEC as a quick online clean-up step that improves both the ruggedness of LC/MS methods and the quality of data obtained. On-line SEC also decreases HPLC column degradation, adding to the robustness of the overall analytical method. Furthermore, on-line clean-up reduces sample preparation, which saves time and minimizes potential analyte loss.

Experimental
All analyses were performed using a Finnigan Surveyor MS Pump and Autosampler and a Finnigan LCQ Deca XP Plus mass spectrometer operated in both full-scan and
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Practical Aspects of Evaluation of Chromatographic Data in Size Exclusion Chromatography
2. Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates using a TSKgel Super SW3000 Size-Exclusion Column
3. Improve Recovery and Reproducibility with Pre-Treatment of Analytical TSK-GEL G3000SWXL Size Exclusion Columns
4. Purification of Murine IgG1 Using UNOsphere S and CHT Ceramic Hydroxyapatite Chromatography, Rev A
5. Rapid Chromatography Optimization by pH Scouting Using the New BioLogic Maximizer Valve System, Rev A
6. Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins
7. Separation Reproducibility With the BioLogic Chromatography Systems
8. Determination of N-nitrosamines in Baby Bottle Rubber Teats by Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry
9. Separation and Quantitation of R and S Enantiomers using Normal Phase Chromatography on an API 3000 LC/MS/MS System
10. Separation of Peptides by Hydrophilic Interaction Chromatography Using TSKgel Amide-80
11. Sample Preparation for Microinjection
Post Your Comments:
(Date:7/24/2014)... , July 24, 2014  ECC West Africa, LLC ... with University College Hospital (UCH) Ibadan Board ... project to finance, design, construct, and operate and maintain ... Nigeria . Under the first of the 4-phase ... documents and funding applications. UCH Ibadan intends to finance ...
(Date:7/24/2014)... scientists are making progress in devising suitable means ... relies on quantum dotsa kind of artificial atom, ... new study demonstrates that changing the coupling of ... impulses can help better control them. This has ... quantum information units, which would produce faster quantum ...
(Date:7/24/2014)... -- Research and Markets has announced the ... report to their offering.  ... in vitro growth of plant or animal cells for ... production. Cell culture is a crucial step in tissue ... culture consumables are necessary for the in vitro production ...
(Date:7/24/2014)... 2014 SRI International has been awarded a ... of Allergy and Infectious Diseases (NIAID), part of the ... potential therapies for HIV infection and AIDS. The contract ... AIDS and the complications and opportunistic infections associated with ... transmission of HIV. According to the ...
Breaking Biology Technology:ECC Signs a Contract for the Finance, Design, and Construction of a Premier Cancer Institute in Ibadan, Nigeria 2Global Cell Culture Market 2014-2018: Key Vendors are Becton, Dickinson and Co, EMD, GE Healthcare, Lonza Group, Sigma Aldrich and Thermo Fisher Scientific 2National Institute of Allergy and Infectious Diseases Awards SRI International Contract to Study New Therapies for HIV and AIDS 2National Institute of Allergy and Infectious Diseases Awards SRI International Contract to Study New Therapies for HIV and AIDS 3
... 2011 Physicians and scientists from Reproductive Medicine ... results of their groundbreaking research study entitled ... Potential While Blastocyst Biopsy Does Not: A Novel ... Embryos.   The study, led by Richard T. ...
... Intellect Neurosciences, Inc. (OTCPK: ILNS) announces that Dr. Daniel G. ... Annual BIO Investor Forum.  Hosted by the Biotechnology Industry Organization ... and emerging public companies. The meeting will take place October ... Chain will be presenting on October 26th at 1:30 p.m. ...
... Oct. 19, 2011 Process automation specialist Endress+Hauser has ... that develops, produces and distributes sensors, hardware and software ... strengthen its position in the area of biotechnology through ... role in the future, not only in the life ...
Cached Biology Technology:Revolutionary IVF Study May Change the Way Embryos are Biopsied for Genetic Disease 2Revolutionary IVF Study May Change the Way Embryos are Biopsied for Genetic Disease 3Intellect Neurosciences, Inc. to Present at 10TH Annual BIO Investor Forum 2Intellect Neurosciences, Inc. to Present at 10TH Annual BIO Investor Forum 3Endress+Hauser and Finesse Solutions Form Strategic Partnership - Swiss Process Automation Provider Invests in US Biotech Company 2Endress+Hauser and Finesse Solutions Form Strategic Partnership - Swiss Process Automation Provider Invests in US Biotech Company 3
(Date:7/24/2014)... cars ply silent freeways, solar panels blanket rooftops and ... from howling winds and from the blazing desert sun. ... technically and economically feasible to convert California,s all-purpose energy ... in Energy , the plan shows the way ... California that could create tens of thousands of jobs ...
(Date:7/24/2014)... of researchers has developed a new nanoscale agent for ... for assessing the function and properties of the GI ... and treatment of gut diseases. , Illnesses ... and inflammatory bowel disease all occur in the intestine ... with diseases such as diabetes and Parkinson,s. , ...
(Date:7/24/2014)... at NJIT this week for an all-day public forum ... Protection Agency (EPA) to dredge toxic sediment from an ... EPA has called the plan, which proposes removing 4.3 ... of the riverbed, one of the largest cleanups in ... and disposed off-site. , "We need to ensure the ...
Breaking Biology News(10 mins):Stanford study shows how to power California with wind, water and sun 2Stanford study shows how to power California with wind, water and sun 3Stanford study shows how to power California with wind, water and sun 4New imaging agent provides better picture of the gut 2Experts Weigh the Pros and Cons of a $1.7 Billion EPA Cleanup Plan for the Passaic River at an NJIT 2
... COLUMBUS, Ohio Retroviruses such as HIV and HTLV-1 don,t ... their own DNA into the cell,s DNA, and from this ... But that infection might be much less troublesome and much ... response to the virus during its first few days in ...
... major 7.0-magnitude earthquake struck the Haitian capital of Port-au-Prince ... quake was followed by several aftershocks with magnitudes over ... maps suddenly out of date, causing additional challenges to ... can help rescue efforts by providing updated views of ...
... The University of Plymouth is to provide 500,000 funding ... of research in partnership with the University, it was announced ... epidemiology in oral health, including the review of published evidence ... of dental research data. The second will look ...
Cached Biology News:Early immune response needed for hit-and-hide cancer viruses 2First satellite map of Haiti earthquake 2First satellite map of Haiti earthquake 3University of Plymouth invests in dental research 2
... loxP-PGK-gb2-neo-loxP template is designed to allow ... cells. The loxP-PGK-gb2-neo-loxP template encodes ... a prokaryotic promoter (gb2) for expression ... a eukaryotic promoter (PGK) for expression ...
... depletion cocktail is tailored to ... from suspensions of rat spleen ... the StemSep (or compatible 0.5 ... system. The CD4+ T cell ...
Standard Fluka For microscopy (Hist.) redox indicator...
... The VSOS-4 Orbital Shaker is designed ... to provide a uniform motion that is both ... The VSOS-4 Orbital Shaker provides a smooth, quiet, ... for a wide range of laboratory applications such ...
Biology Products: