Navigation Links
Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor

says 5-HT appears as a full agonist, GR127935 acts as a partial agonist and SB-224289 appears as an inverse agonist. The maximum response of 5-HT and GR127935 differs between the two methods (Table 1), but the intrinsic activity of GR127935 is comparable. The maximum response produced by SB-224289 is similar for both assay systems. The comparison between the two methods is summarized in Table 2.

Discussion

This studys aim was to investigate the efficacy of PerkinElmers Basic FlashPlate as a tool for measuring [35S]-GTPγS binding, using CHO cells expressing the human 5-HT1B receptor as the receptor source. After optimal conditions were assessed, significant stimulation of binding was achieved in the presence of an agonist. The FlashPlate system was also sensitive enough to pick up partial and inverse agonists. (Assessment of optimal conditions will be essential when designing other assays on FlashPlate, for different receptors expressed in different cell lines.)

The FlashPlate-based method agreed closely with the conventional filtration method in terms of potencies and intrinsic activities of the compounds tested, but differences in maximum responses were seen. Intra- and inter-assay variation present in the FlashPlate system may play a role in this, and might be attributable to non-uniform coating of membrane protein to wells. The pipetting procedure adopted, or the volume of residual reaction mixture after aspiration may also be factors. This may not be a major concern if the window of response is adequate for the given application. In cases where intra- and inter-assay variation is a problem, it might be addressed by performing assays in duplicate or greater multiples.

Significantly, total assay time was reduced from 12 hours for the filtration method to 4 hours on FlashPlate. Labor time was also reduced significantly. The FlashPlate method also allows greater s
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Counting FlashPlates on the Wallac MicroBeta
2. Use of Novel FlashPlate Technology to Measure cAMP Accumulation in Chinese Hamster Ovary Cells Expressing Human -2 Adrenoreceptors
3. Use of FlashPlate Technology for In Vitro Measurement of 125I-Labeled TGF-1 Binding on Chimeric Extracellular Domain of Type II Transforming Growth Factor Receptor
4. In Vitro Measurement of the Second Messenger cAMP: RIA vs. FlashPlate
5. Evaluation of FlashPlate in a Helicase Assay
6. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
7. Enhancing Ruggedness and Full-Scan MS Sensitivity Using Ion Sweep Technology
8. Novel MicroRNA Array Technology for Sensitive miRNA Profiling
9. DNA purification from pig ears to bird blood using proven DNeasy Technology
10. Near Infrared Technology and Optical Agents For Molecular Imaging
11. In-Vitro Metabolism Studies Using Data-Dependent LC/MSn
Post Your Comments:
(Date:9/30/2014)... Follow us on LinkedIn –Cholesterol ... cholesterol carrier or lipoprotein in the blood. Such tests ... in the body, especially along the walls of blood ... other cardiovascular disorders. Cardiovascular Diseases (CVD) is the leading ... on a regular basis, to assess an individual’s risk ...
(Date:9/30/2014)... 2014 CORD:USE Cord Blood Bank – ... has entered into an Equity and Exclusive Services Agreement ... series of patented technologies for the isolation of human ... Tianhe is performing Phase I/II clinical trials in ... using Tianhe,s Stem Cell Educator Therapy showing that ...
(Date:9/30/2014)... 30, 2014 Genedata, a ... drug discovery and related life science research, ... chosen Genedata Selector as its host-pathogen data ... FUNGITECT is a European consortium dedicated to ... via individualized anti-fungal drug therapies. Genedata Selector ...
(Date:9/30/2014)... 30, 2014  KemPharm, Inc., a clinical-stage specialty ... of proprietary new molecular entity (NME) prodrugs, announced ... Office (USPTO) issued U.S. Patent No. 8,816,083 to ... Benzoic Acid Derivatives and Heteroaryl Carboxylic Acid Conjugates ... Thereof." The patent, which extends through ...
Breaking Biology Technology:Surging Diabetic, Obese and Hypertensive Population Drives the Global Cholesterol Testing Market, According to New Report by Global Industry Analysts, Inc. 2Surging Diabetic, Obese and Hypertensive Population Drives the Global Cholesterol Testing Market, According to New Report by Global Industry Analysts, Inc. 3Surging Diabetic, Obese and Hypertensive Population Drives the Global Cholesterol Testing Market, According to New Report by Global Industry Analysts, Inc. 4CORD:USE is Pleased to Announce it has Partnered with Tianhe Stem Cell Biotechnologies, Developer of Cord Blood Stem Cell Therapy Showing Promising Results in Clinical Trials for the Treatment of Diabetes and Autoimmune Diseases 2CORD:USE is Pleased to Announce it has Partnered with Tianhe Stem Cell Biotechnologies, Developer of Cord Blood Stem Cell Therapy Showing Promising Results in Clinical Trials for the Treatment of Diabetes and Autoimmune Diseases 3CORD:USE is Pleased to Announce it has Partnered with Tianhe Stem Cell Biotechnologies, Developer of Cord Blood Stem Cell Therapy Showing Promising Results in Clinical Trials for the Treatment of Diabetes and Autoimmune Diseases 4FUNGITECT Consortium Adopts Genedata Selector as Data Analysis Platform for Anti-fungal R 2FUNGITECT Consortium Adopts Genedata Selector as Data Analysis Platform for Anti-fungal R 3KemPharm, Inc. Receives Patent from the USPTO for Novel Prodrug of Hydromorphone, KP511 2
... Lund University in Sweden have discovered a new stem cell ... several different cell types -- most importantly, they can form ... finding to develop methods to heal and repair disease and ... biopsies, the researchers for the first time found stem cells ...
... April 19, 2012  Phigenics LLC, an innovative water management ... paper that demonstrates up to 33% false-positive test results ... methods. A false-positive result means the test indicated ... detectable levels when in fact it was below detectable ...
... Biofuels, LLC, a joint venture between BP and ... collaboration with Fagen, Inc. for the introduction of ... the world,s most experienced biofuels engineering, procurement and ... any other company.  They are the largest, most ...
Cached Biology Technology:New Stem Cell Found in the Brain 2New Stem Cell Found in the Brain 3Phigenics Research Challenges Accuracy Of Conventional Legionella Testing Protocol 2Phigenics Research Challenges Accuracy Of Conventional Legionella Testing Protocol 3Butamax™ and Fagen Announce Collaboration to Retrofit Ethanol Plants for Biobutanol Production 2
(Date:9/30/2014)... Researchers of Ludwig-Maximilians-Universitaet (LMU) in Munich report that ... cells more sensitive to chemotherapeutic drugs. They have ... a new target for anti-tumor agents. , ... Professor Stephan Sieber of the Technische Universitt Mnchen ... a potential new weapon in the fight against ...
(Date:9/30/2014)... 2014  Spectra Automation, a trusted supplier ... and power generation industries, announced today the ... an easy-to-use and cost-effective data collection, analysis ... laboratories. Most upstream process ... systems. Accessing and consolidating data from these ...
(Date:9/29/2014)... of gut bacteria called Clostridium ramosum , coupled with ... work is published this week in mBio , the ... , A research team from the German Institute of Human ... bacteria including C. ramosum gained weight when fed ... ramosum were less obese even when consuming a high-fat ...
Breaking Biology News(10 mins):Cancer therapy: Driving cancer cells to suicide 2Spectra Automation Introduces RECONN Bioprocess Manager 2Gut bacteria promote obesity in mice 2
... deposition associated with industry and agriculture can drive soils ... and polluting surface waters, according to a new study ... study, conducted in the Tatra Mountains of Slovakia by ... of Sciences, and the U.S. Geological Survey, shows what ...
... both physically and mentally, but genetically everyone is ... for decades now. But with population research becoming ... Tim Caulfield is concerned that genetic research could ... Nobel Prize winning geneticist James Watson claimed there ...
... new study by researchers at UC Davis Medical Center ... patients may be a result of their brains not ... drops in blood oxygen levels are more common than ... senior author Masud Seyal, a professor of neurology at ...
Cached Biology News:Acid soils in Slovakia tell somber tale 2Could genetic research awaken racist attitudes? 2Drops in blood oxygen levels may be key to sudden death in some epilepsy patients 2Drops in blood oxygen levels may be key to sudden death in some epilepsy patients 3
Mouse monoclonal antibody raised against a partial recombinant TPR. NCBI Entrez Gene ID = TPR...
... sample is a complex protein sample, ... when used with ReadyPrep 2-D starter ... sample can be used as a ... and protocol before using more difficult ...
... materials for 1,464 array sample elements. , ,Includes: ... , Blocker BSA in PBS (10X): 50 ml ... g , BupH Phosphate Buffered Saline: ... 2 x 10 ml ampules , SuperSignal West Pico ...
... Mouse polyclonal antibody raised ... Immunogen: ... 112 a.a) partial recombinant protein ... Accession Number: NM_130777 ...
Biology Products: