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Use of BRET2 luminescence cell-based assays with LEADseeker Multimodality Imaging System

Key words: luminescence • BRET2 • LEADseeker • DeepBlueC• cell-based assays


Competitive pressure is forcing organizations to continually increase screening throughput. Yet accuracy and sensitivity remain vital concerns, particularly for common screens such as luminescence cell-based assays. LEADseeker™ Multimodality Imaging System was developed to meet these demands by combining excellent detection with the ability to image whole plates in less than one minute.

This application note describes the use of luminescence emissions filters, which were defined and characterized using the BRET2™ Demonstration Assay Kit. The data demonstrate that BRET2 cell-based assays perform well in conjunction with the chemiluminescent readout of LEADseeker Multimodality Imaging System. Together, the filters, demonstration kit, and assay system support organizations looking to add high and ultra-high throughput luminescence cell based assays to their screening programs.


Materials
Products used
LEADseeker Multimodality Imaging System 18-1140-71

Luminescence filter configuration for Bret2 consists of: 11-0013-49

Emission filter (Luciferin donor) 470 nm, bp 50 nm

Emission filter (YFP acceptor) 535 nm, bp 45 nm


Other materials required
BRET2 Demonstration Assay Kit (PerkinElmer)

DeepBlueC™ (PerkinElmer)

384-well plates (non-treated, non-sterile, polystyrene, white)


Method
1. Reagent preparation
1.1 Prepare reagents as recommended by the kit manufacturer. Reagents,
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