Navigation Links
Use of BRET2 luminescence cell-based assays with LEADseeker Multimodality Imaging System

Key words: luminescence • BRET2 • LEADseeker • DeepBlueC• cell-based assays


Competitive pressure is forcing organizations to continually increase screening throughput. Yet accuracy and sensitivity remain vital concerns, particularly for common screens such as luminescence cell-based assays. LEADseeker™ Multimodality Imaging System was developed to meet these demands by combining excellent detection with the ability to image whole plates in less than one minute.

This application note describes the use of luminescence emissions filters, which were defined and characterized using the BRET2™ Demonstration Assay Kit. The data demonstrate that BRET2 cell-based assays perform well in conjunction with the chemiluminescent readout of LEADseeker Multimodality Imaging System. Together, the filters, demonstration kit, and assay system support organizations looking to add high and ultra-high throughput luminescence cell based assays to their screening programs.


Materials
Products used

LEADseeker Multimodality Imaging System 18-1140-71

Luminescence filter configuration for Bret2 consists of: 11-0013-49

Emission filter (Luciferin donor) 470 nm, bp 50 nm

Emission filter (YFP acceptor) 535 nm, bp 45 nm


Other materials required
BRET2 Demonstration Assay Kit (PerkinElmer)

DeepBlueC™ (PerkinElmer)

384-well plates (non-treated, non-sterile, polystyrene, white)


Method
1. Reagent preparation
1.1 Prepare reagents as recommended by the kit manufacturer. Reagents,
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
2. Photoluminescence Spectroscopy of Quantum Dots, monitored by the Spex FluoroMax
3. Time gated separation of lanthanide luminescence
4. A highly sensitive ATP-luminescence assay using VICTOR2 with the dispenser option
5. LiquiChip Kits for bead-based cytokine and kinase assays
6. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
7. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
8. Is Z factor the best assessment for the quality of cellular assays delivering higher content?
9. The measurement of DNA condensation and mitochondrial changes during apoptosis and necrosis in cell based assays
10. Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module
11. Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System
Post Your Comments:
(Date:8/28/2015)... ... 2015 , ... Since the initial launch of FireflySci Inc. (FFS) in early 2015, the company ... Images such as the Fonz in a lab coat and Large Marge have been ... spectroscopy field. , FireflySci is proud to be the only cuvette manufacturer is ...
(Date:8/27/2015)... - Portage Biotech Inc. ("Portage") (OTC Market: PTGEF, ... Company Limited (Biohaven), announced today that dosing has ... a glutamate modulating agent. Biohaven filed an investigational ... clearance from the U.S. Food and Drug Administration ... 54% equity interest in Biohaven, a private company. ...
(Date:8/27/2015)... SAN DIEGO , Aug. 27, 2015 ... second annual meeting of the Japan Society of HTLV-1, ... Science Institute Auditorium. The purpose of the Society is ... well as the development of medical technology and research ... health and welfare. "HUYA is proud to ...
(Date:8/27/2015)... Aug. 27, 2015 In response to ... UPS and The US Postal Service to no ... as ,select agents, by the CDC, Marken reaffirmed ... these sensitive shipments. Marken uses its ... trained and certified personnel to ensure the safe ...
Breaking Biology Technology:Cuvette Manufacturer FireflySci Launches Back to School Deals with Complimentary Shipping On All Orders 2Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 2Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 3Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 4HUYA Bioscience International Sponsors Japan Society Of HTLV-1 Annual Meeting 2HUYA Bioscience International Sponsors Japan Society Of HTLV-1 Annual Meeting 3HUYA Bioscience International Sponsors Japan Society Of HTLV-1 Annual Meeting 4Marken Ensures Safe Transportation Of Select Agents And Toxins 2
... have hit universities and colleges across the country, ... alleged copyright infringement of both movies and music ... sent several cease-and-desist notices to the Universirty of ... apparently were hosting movie downloads with content that ...
... begin three weeks of special articles, interviews and ... Network (WTN). This series called, "FORWARD VISIONS" is ... will help to illustrate some of the great ... Technology Recognition Week, November 1-7. WTN will publish ...
... agricultural and industrial revolutions. And today we stand ... economy; the innovation revolution. If we harness our resources ... coming innovation economy. , ,President George W. Bush ... the state and local government levels must also make ...
Cached Biology Technology:File Swapping: Download at your own Risk 2File Swapping: Download at your own Risk 3Why Wisconsin Lags Behind the U.S. and Minnesota 2Why Wisconsin Lags Behind the U.S. and Minnesota 3Why Wisconsin Lags Behind the U.S. and Minnesota 4Why Wisconsin Lags Behind the U.S. and Minnesota 5Broadband Deployment & Adoption in Wisconsin 2Broadband Deployment & Adoption in Wisconsin 3
(Date:8/4/2015)... AMRI ) today reported financial and operating results for the ... Second quarter contract revenue of $ 85.2 ... contract margins of 26 % , Second ... EPS from royalties in the current quarter , ... very pleased to present another strong financial quarter, with all our ...
(Date:7/31/2015)... julho de 2015 A 10 a. ... realizada pela BGI de 22-25 de outubro de 2015, em ... A conferência está celebrando seu 10 o. aniversário este ... tornou uma das reuniões mais influentes do mundo no ... dinâmicos, entusiastas e prazerosos. A ICG-10 ...
(Date:7/31/2015)... , Jul. 31, 2015 NXT-ID, Inc. (NASDAQ: ... authentication company focused on the growing mobile commerce market ... it has filed provisional patent 62/198989 for ... invention highlights a method to advance crypto-currencies such as ... introducing a common, uniform way to manage all payments.  ...
Breaking Biology News(10 mins):AMRI Announces Second Quarter 2015 Results 2AMRI Announces Second Quarter 2015 Results 3AMRI Announces Second Quarter 2015 Results 4AMRI Announces Second Quarter 2015 Results 5AMRI Announces Second Quarter 2015 Results 6AMRI Announces Second Quarter 2015 Results 7AMRI Announces Second Quarter 2015 Results 8AMRI Announces Second Quarter 2015 Results 9AMRI Announces Second Quarter 2015 Results 10AMRI Announces Second Quarter 2015 Results 11AMRI Announces Second Quarter 2015 Results 12AMRI Announces Second Quarter 2015 Results 13AMRI Announces Second Quarter 2015 Results 14AMRI Announces Second Quarter 2015 Results 15AMRI Announces Second Quarter 2015 Results 16AMRI Announces Second Quarter 2015 Results 17AMRI Announces Second Quarter 2015 Results 18AMRI Announces Second Quarter 2015 Results 19AMRI Announces Second Quarter 2015 Results 20AMRI Announces Second Quarter 2015 Results 21AMRI Announces Second Quarter 2015 Results 22AMRI Announces Second Quarter 2015 Results 23AMRI Announces Second Quarter 2015 Results 24A 10a. Conferência Internacional sobre Genômica (ICG-10) será em outubro 2A 10a. Conferência Internacional sobre Genômica (ICG-10) será em outubro 3NXT-ID Patents Electronic Crypto-Currency Management Technology 2NXT-ID Patents Electronic Crypto-Currency Management Technology 3NXT-ID Patents Electronic Crypto-Currency Management Technology 4
... of breast cancer just because a woman is pregnant, said ... Group. This suggestion is based on study results detailing ... presented this data at the 33rd Annual CTRC-AACR San Antonio ... time we started the study in 2003, there was hardly ...
... of some of the most economically damaging organisms that infect ... that causes powdery mildew and the water molds that cause ... cruciferous vegetables and other crops. , "We have been studying ... Kamoun, head of the Sainsbury Laboratory on the Norwich Research ...
... the original sweetener, manufactured by honey bees long before ... honey gatherers, as do ancient Egyptian reliefs. From Mesopotamia ... nearly every human culture and cuisine. "Honey is ... its unbelievable activity. It,s just an amazing substance," said ...
Cached Biology News:Unique needs and outcomes of pregnant women with breast cancer identified 2Gene hunters tackle crop diseases 22 books explore the history and delights of honey, bees and beehives 22 books explore the history and delights of honey, bees and beehives 32 books explore the history and delights of honey, bees and beehives 4
... Doublestain System is a high-sensitivity peroxidase ... The kit is intended for use ... of two different antigens within the ... suitably diluted rabbit and mouse primary ...
Anti-TNKS2 Polyclonal AntibodyOther Sub-Family: not assigned-Other Applications: ELISA, Western Blot...
... Recognizes VAMP-2 (Vesicle-Associated Membrane Protein ... does not share homology with ... It is expected that ... with human, Macaca multata and ...
... two unrelated proteins both of which function ... cycle in a p53-dependent manner. p14^ARF^ binds ... of mdm2 proteinrequired for mdm2 modification and ... interaction is mediated by the exon 1Beta-encoded ...
Biology Products: