Navigation Links
Uracil-DNA Glycosylase

for prevention of carry-over contamination in PCR Cat. No. 1 444 646 100 units
Cat. No. 1 269 062 25 units Description Uracil-DNA Glycosylase (UNG) (from E. coli) catalyzes the removal of uracil from single- and double-stranded DNA that has been synthesized in the presence of dUTP. The apyriminic sites formed by UNG are susceptible to cleavage by heat under alkaline conditions (carry-over prevention). UNG will not digest 3'-terminal dU, dUTP, primers labeled with biotin-dUTP, or digoxigenin-dUTP. Ribouracil residues in RNA are also unaffected by UNG. Application Prevention of carry-over contamination in:
  • PCR with Taq DNA Polymerase.
  • RT-PCR with C. therm. Polymerase One-step RT-PCR System.
Operating parameters
  • Substrate: Single- or double-stranded DNA containing deoxyuracil (DNA synthesized in the presence of dUTP).
  • Incubation conditions for cleavage of uracil-glycosidic bonds: 20C, 110 min.
  • Thermal stability (pH 8.3): half-life at 40C, 2 min; half-life at 45C, 0.5 min.
  • MgCl2 concentration: 2.5 mM.
  • dNTP concentration: 200 M dNTPs; 600 M dUTP.
  • Conditions for inactivation of enzyme: 95C, 2 min.

Notes:

UNG (2 units) will degrade up to 106 uracil-containing DNA molecules in 10 min. In contr
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Preventing Carry-over Contamination with Uracil-DNA Glycosylase
2. Uracil-DNA Glycosylase, heat-labile
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Uracil DNA Glycosylase

(Date:12/11/2009)... -- Dendreon Corporation (Nasdaq: DNDN ) announced ... public offering have exercised in full their option ... stock at the public offering price of $24.75 ... increases the size of the offering to an ... Dendreon expects to receive net proceeds from the ...
(Date:12/11/2009)... have discovered a new explanation for differences in the severity ... a certain gene, the more serious the handicap. The genetic ... that it is the amount of copies of the GDI1 ... the American Journal of Human Genetics , and are ... connected to VIB, a life sciences research institute in Flanders, ...
(Date:12/11/2009)... -- Ambit Biosciences Corporation announced today the enrollment and ... Cancer Center of the first patient in the ACE ... fficacy) Phase 2 pivotal trial in patients with ... is a novel, orally available, potent and highly selective ... tyrosine kinase-3 (FLT3) inhibitor. The ACE study is ...
(Date:12/11/2009)... 11 Marrone Bio Innovations (MBI) today ... team. Mr. Chris Hildreth joined MBI as Senior ... for expanding all commercial aspects of the company, ... Kin Sing (Ray) Lam joined MBI as Vice ... development efforts in scaling its broad pipeline of ...
Breaking Biology Technology:Dendreon Announces Exercise of Option by Underwriters in its Common Stock Offering 2Dendreon Announces Exercise of Option by Underwriters in its Common Stock Offering 3Amount of gene surplus determines severity of mental retardation in males 2Ambit Biosciences Initiates First Phase 2 Clinical Trial of AC220 in Acute Myeloid Leukemia 2Ambit Biosciences Initiates First Phase 2 Clinical Trial of AC220 in Acute Myeloid Leukemia 3Ambit Biosciences Initiates First Phase 2 Clinical Trial of AC220 in Acute Myeloid Leukemia 4Ambit Biosciences Initiates First Phase 2 Clinical Trial of AC220 in Acute Myeloid Leukemia 5Marrone Bio Innovations Announces Two New Executive Appointments 2Marrone Bio Innovations Announces Two New Executive Appointments 3
... the global specialty biopharmaceutical company,announces results for the three months to March 31, 2009. , Financial Highlights, ... ... ... Product sales (excluding ADDERALL XR) $460 million ...
... by Microfluidics and Imaging -- Net Loss Narrowed by $0.07 ... Inc. (Nasdaq: CALP ) today reported its first ... $28.5 million compared to $29.3 million in the first quarter ... of 2008 included $2.9 million of revenue related to product ...
... and VANCOUVER, April 30 /PRNewswire-FirstCall/ - OncoGenex Pharmaceuticals (NASDAQ: ... first quarter financial results will be released on Thursday ... a conference call and live webcast at 4:30 p.m. ... quarter results and provide an update on the business ...
Other Biology Technology:Shire begins the year with a strong performance 2Shire begins the year with a strong performance 3Shire begins the year with a strong performance 4Shire begins the year with a strong performance 5Shire begins the year with a strong performance 6Shire begins the year with a strong performance 7Shire begins the year with a strong performance 8Shire begins the year with a strong performance 9Shire begins the year with a strong performance 10Shire begins the year with a strong performance 11Shire begins the year with a strong performance 12Shire begins the year with a strong performance 13Shire begins the year with a strong performance 14Shire begins the year with a strong performance 15Shire begins the year with a strong performance 16Shire begins the year with a strong performance 17Shire begins the year with a strong performance 18Shire begins the year with a strong performance 19Shire begins the year with a strong performance 20Shire begins the year with a strong performance 21Shire begins the year with a strong performance 22Shire begins the year with a strong performance 23Shire begins the year with a strong performance 24Shire begins the year with a strong performance 25Shire begins the year with a strong performance 26Shire begins the year with a strong performance 27Shire begins the year with a strong performance 28Shire begins the year with a strong performance 29Shire begins the year with a strong performance 30Shire begins the year with a strong performance 31Shire begins the year with a strong performance 32Shire begins the year with a strong performance 33Caliper Life Sciences Reports First Quarter 2009 Results 2Caliper Life Sciences Reports First Quarter 2009 Results 3Caliper Life Sciences Reports First Quarter 2009 Results 4
(Date:12/11/2009)... How does a cell manage to identify and degrade ... the body against serious diseases? The researchers Sabine C. ... Ernst Jarosch of the Max Delbrck Center for Molecular ... piece in this puzzle. In an enzyme complex that ... proteins, they discovered a scaffold regulating the identification and ...
(Date:12/11/2009)... about the deep ocean,s temperature variability and circulation system ... The deep ocean is affected more by surface warming ... accurate predictions of factors such as sea level rise ... have also been found to result in a more ... a faster transport of large quantities of warm water, ...
(Date:12/11/2009)... moderate amount of weight by eating less and exercising ... Washington University School of Medicine in St. Louis. , ... Dec. 15, 2009, issue of the Journal of ... loss led to improvement in four key measures of ... study participants included decreased thickness of heart muscle, improved ...
Breaking Biology News(10 mins):MDC researchers identify a scaffold regulating protein disposal 2New discoveries could improve climate projections 2Moderate weight loss in obese people improves heart function 2Moderate weight loss in obese people improves heart function 3Mediterranean Diet May Be Best for Type 2 Diabetes 55765 1Mediterranean Diet May Be Best for Type 2 Diabetes 55765 2Mediterranean Diet May Be Best for Type 2 Diabetes 55765 3JFKs Health Problems More Complex Than Thought 55762 1JFKs Health Problems More Complex Than Thought 55762 2JFKs Health Problems More Complex Than Thought 55762 3Abbott to Present at Morgan Stanley Global Healthcare Unplugged Conference 55759 1
... Turn up the white noise, says a team of ... the benefits of noise. Their findings could lead to ... an assistant professor of computer science, Joseph Martin, a ... of biology, are working together to explain the biological ...
... mass index, or BMI, long considered the standard for measuring ... be as accurate as originally thought, according to new research. ... Valley State University measured the BMI of more than 400 ... ?and found that in most cases the student's BMI did ...
... but for those with arousal system disturbances in their brains, ... could sense they were really outside their own body watching ... 6 percent said they have had such an out-of-body experience. ... University of Kentucky have studied the link between out-of-body experiences, ...
Other Biology News:Noise echoes in cell communications 2BMI not accurate indicator of body fat 2Out-of-body experiences may be caused by arousal system disturbances in brain 2
Apoptosis Detection Systems and Reagents...
Barcoded Slides, 5 Packs of 5 Slides (25/Case)...
Takara's Mutan-Super Express Km Kit is designed to achieve site-directed mutagenesis in just one day. It is based on Oligonucleotide-directed Dual Amber (ODA) method, using the advantages of LA (Long...
...
Biology Products: