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Unmask Blood RNA for Gene Expression Profiling

samples with the RiboPure-Blood Kit (Ambion). While half of the samples were processed with the GLOBINclear Kit before RNA amplification (MessageAmp II Kit, Ambion), half of the total RNA preparations were used directly for RNA amplification. Labeled aRNA was hybridized to an Affymetrix GeneChip Human Genome Focus Array. Percent Present Calls were ~10% (~1000 genes) lower for samples that were not treated with the GLOBINclear Kit.


Globin mRNA levels can vary among donors, which can unpredictably affect RNA amplification and array results and therefore, confound interpretations that are made when comparing different samples. With the removal of globin transcripts, the enriched RNA samples become superior templates for RNA expression profiling because the presence of globin mRNA is no longer a factor in decreasing the sensitivity of gene detection on microarrays (Figure 2).


GLOBINclear-Human Globin mRNA Removal Kit

The GLOBINclear protocol is compatible with total RNA obtained from any high quality whole blood RNA isolation procedure. This kit was developed to reduce or eliminate the negative effects of globin mRNA on high density oligonucleotide expression profiling, and the resulting enriched RNA is compatible with the RiboPure-Blood Kit and the MessageAmp II aRNA Amplification System. The GLOBINclear Kit contains enough reagents to deplete globin mRNA from 20 samples of total RNA derived from human whole blo
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