Navigation Links
Unmask Blood RNA for Gene Expression Profiling

samples with the RiboPure-Blood Kit (Ambion). While half of the samples were processed with the GLOBINclear Kit before RNA amplification (MessageAmp II Kit, Ambion), half of the total RNA preparations were used directly for RNA amplification. Labeled aRNA was hybridized to an Affymetrix GeneChip Human Genome Focus Array. Percent Present Calls were ~10% (~1000 genes) lower for samples that were not treated with the GLOBINclear Kit.


Globin mRNA levels can vary among donors, which can unpredictably affect RNA amplification and array results and therefore, confound interpretations that are made when comparing different samples. With the removal of globin transcripts, the enriched RNA samples become superior templates for RNA expression profiling because the presence of globin mRNA is no longer a factor in decreasing the sensitivity of gene detection on microarrays (Figure 2).


GLOBINclear-Human Globin mRNA Removal Kit

The GLOBINclear protocol is compatible with total RNA obtained from any high quality whole blood RNA isolation procedure. This kit was developed to reduce or eliminate the negative effects of globin mRNA on high density oligonucleotide expression profiling, and the resulting enriched RNA is compatible with the RiboPure-Blood Kit and the MessageAmp II aRNA Amplification System. The GLOBINclear Kit contains enough reagents to deplete globin mRNA from 20 samples of total RNA derived from human whole blo
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
3. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
4. EppendorfPerfect gDNA Blood Mini Kit
5. Higher gDNA Concentrations with the Perfect gDNA Blood Mini Kit by Varying the Final Elution Volume
6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
7. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
8. Higher gDNA Concentrations with the Perfect gDNA Blood Mini Kit by Varying the Final Elution Volume
9. EppendorfPerfect gDNA Blood Mini Kit
10. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
11. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Unmask Blood RNA for Gene Expression Profiling

(Date:8/29/2014)... 29, 2014 Three Lawrence Livermore ... Hydrogen Production R&D Award for their ... water using sunlight. , Shared with collaborators from ... University of Nevada, Las Vegas (UNLV), the award ... of photoelectrochemical solar-hydrogen production and corrosion processes. , ...
(Date:8/28/2014)... August 28, 2014 SPIE Fellow Glenn ... and Optical Science and Director of the Center for ... Carolina at Charlotte, and co-founder and Chairman of the ... as the 2015 Vice President of SPIE, the international ... Philip Stahl announced recent election results at the Annual ...
(Date:8/28/2014)... A new research platform uses a ... tiny structures undergoing stress and heating, an ... designs for microelectronics and batteries., This new ... about how heating and the surface stress ... Researchers have discussed the merits of surface-stress ...
(Date:8/28/2014)... Aug. 28, 2014  Next month, executives from clinical trial ... industry events beginning with Patient-Centered Clinical Trials 2014 , ... Boston , September 4-5. Patient recruitment experts ... Aaron Fleishman will share insights on the benefits of ... of tactics – from media to mobile apps – can ...
Breaking Biology Technology:Livermore Team Awarded for Hydrogen Production Research 2Glenn Boreman, UNC Professor and Plasmonics Co-Founder, Elected to SPIE Presidential Chain 2New analytical technology reveals 'nanomechanical' surface traits 2New analytical technology reveals 'nanomechanical' surface traits 3BBK Worldwide Leads Sessions at Key September Events 2
... Webcast on Wednesday, June 17 at 8:30 a.m. Eastern Time ... Savient Pharmaceuticals, Inc. (Nasdaq: SVNT ) announced today ... and Drug Administration (FDA) recommended by a vote of ... uricase enzyme, be granted marketing approval by the FDA for ...
... ... in a dynamic and unpredictable world at Wharton Leadership Conference. , ... Philadelphia, PA (Vocus) June 16, 2009 -- As the ... is well-positioned to deliver dynamic innovations to meet the evolving markets demand because “change is ...
... , , - Dosimetry Study Results Show Greater ... Normal Organs - , , - All Patients in the ... Time for Recurrent GBM Patients - , , TORONTO and ... PPHM ) today reported that researchers will present data at the ...
Cached Biology Technology:FDA Appointed Arthritis Advisory Committee Recommends U.S. Food and Drug Administration Approval for KRYSTEXXA(TM) for Refractory Chronic Gout 2FDA Appointed Arthritis Advisory Committee Recommends U.S. Food and Drug Administration Approval for KRYSTEXXA(TM) for Refractory Chronic Gout 3FDA Appointed Arthritis Advisory Committee Recommends U.S. Food and Drug Administration Approval for KRYSTEXXA(TM) for Refractory Chronic Gout 4Leading for Growth in the New Economic Reality Includes Vision and Innovation 2Leading for Growth in the New Economic Reality Includes Vision and Innovation 3Data Presented at Society of Nuclear Medicine 2009 Annual Meeting Supports Potential of Peregrine's Cotara(R) for the Treatment of Brain Cancer 2Data Presented at Society of Nuclear Medicine 2009 Annual Meeting Supports Potential of Peregrine's Cotara(R) for the Treatment of Brain Cancer 3Data Presented at Society of Nuclear Medicine 2009 Annual Meeting Supports Potential of Peregrine's Cotara(R) for the Treatment of Brain Cancer 4Data Presented at Society of Nuclear Medicine 2009 Annual Meeting Supports Potential of Peregrine's Cotara(R) for the Treatment of Brain Cancer 5Data Presented at Society of Nuclear Medicine 2009 Annual Meeting Supports Potential of Peregrine's Cotara(R) for the Treatment of Brain Cancer 6
(Date:9/1/2014)... Conventional wisdom has long held that corals ... reefs are passive organisms that rely entirely ... as nutrients and oxygen. But now scientists at ... in Israel have found that they are far ... into turbulent patterns that greatly enhance their ability ...
(Date:9/1/2014)... (September 1, 2014, 10:20 AM EDT) It may ... low-calorie foods over unhealthy higher-calorie foods, according to new ... Nutrition Research Center on Aging (USDA HNRCA) at Tufts ... in the journal Nutrition & Diabetes , a ... that it is possible to reverse the addictive power ...
(Date:9/1/2014)... electron microscopy revealed the presence of tiny blob-like structures ... scientists still don,t know what they do even ... to be crucial to the life of a cell, ... treatment., In the Journal of Cell Biology , ... various backgrounds, from biophysics to cell biology, to focus ...
Breaking Biology News(10 mins):Nature's tiny engineers 2Nature's tiny engineers 3Training your brain to prefer healthy foods 2Scientists call for investigation of mysterious cloud-like collections in cells 2
... scientists have received new funding for a research program ... economically feasible. The project is based on the serendipitous ... fish cages in Puerto Rico by the thousands. The ... fascinating habits such as their tendency to crawl in ...
... mussels and oysters are important vehicles for the ... undercooked. Vibrio species, including human pathogens, are particularly ... even after cleaning procedures, thus representing a potential ... are well known to affect the persistence of ...
... during the summer in Alaska waters, is one of the ... in the 1800s has now left us with only a ... different species from right whales seen in the North Atlantic ... areas and ecological parameters critical to the survival of this ...
Cached Biology News:New project aims to make large-scale lobster farms feasible 2New project aims to make large-scale lobster farms feasible 3Edible bivalves as a source of human pathogens: signals between vibrios and the bivalve host. 2Habitat use by North Pacific right whales, Eubalaena japonica, in the Bering Sea and Gulf of Alaska 2
... is for 2-D separations. The cell has a ... II xi multi-cell can be customized for several ... and cooling system. The PROTEAN II xi multi-cell ... XL multi-cell for a wider format that accommodates ...
... depletion cocktail is tailored to ... suspensions of rat spleen cells. ... StemSep (or compatible 0.5 Tesla) ... T cell content of the ...
... 20 cm, is used for vertical slab gel ... gels. The cell is supplied without spacers and ... with gaskets, lower buffer chamber, lid with cables, ... upper buffer dam, casting stand with gaskets, leveling ...
...
Biology Products: