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Unmask Blood RNA for Gene Expression Profiling

GLOBINclear-Human Globin mRNA Removal Kit
Depletes alpha and beta globin mRNA from blood total RNA with a simple magnetic bead procedure (no enzymatic treatments)

Increases sensitivity of gene detection in microarray profiling experiments

Integrates easily with the RiboPure-Blood Kit and MessageAmp II aRNA Amplification System for expression profiling


Total RNA from Whole Blood

The easy accessibility of blood makes it the most common tissue studied in clinical research, but blood consists of a heterogeneous population of cells, including erythrocytes, granulocytes, and other peripheral blood mononuclear cells. One obstacle for expression profiling RNA from whole blood is the relatively high content of globin messenger RNA (mRNA) from immature red blood cells. In fact, up to 70% of the mRNA mass is from globin transcripts.


Globin Transcript Removal

Ambion's GLOBINclear-Human Kit is designed to rapidly deplete alpha and beta globin mRNA from total RNA preparations derived from human whole blood. The kit employs a novel hybridization capture technology (see "Simple, Effective Globin mRNA Reduction Protocol" at right) that uses the power of biotin/streptavidin binding and the specificity of nucleic acid hybridization to effectively remove 9099.5% globin transcripts from blood total RNA (Figure 1).

Figure 1.'"/>

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