Navigation Links
Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins

ODS-100V than on the ODS-100Z column. Also, separation of nicotinamide and nicotinic acid was enhanced on the more polar column.


Analysis of Lipid Soluble Vitamins
Analysis of vitamin D2 (ergocalciferol) and D3 (cholecalciferol) is critical because they differ only in one methylene group and one double bond. As shown in Figure 2, separation was achieved under isocratic conditions in 100% acetonitrile at a flow rate of 1.0mL/min. The influence of temperature on resolution was evaluated at 25C and 40C.


For this separation TSKgel ODS-100Z, which has higher % carbon than TSKgel ODS-100V, provides better resolution. Ergocalciferol and cholecalciferol elute from both columns with near baseline separation. On the TSKgel ODS-100V column, hydrophobic retention is not high enough for a baseline separation of both vitamin D compounds. On both columns, separation is enhanced at lower temperature (25C).


Simultaneous Separation of Water and Lipid Soluble Vitamins
Water and lipid soluble vitamins were separated in one single run on TSKgel ODS-100V and TSKgel ODS-100Z as demonstrated in Figure 3 .


To separate the mixture of six water-soluble vitamins, caffeine, and four fat-soluble vitamins, required a gradient from water containing 0.1% TFA (eluent A) to acetonitrile containing 0.1% TFA (eluent B). Flow rate was 1mL/min, at a temperature of 40C. To elute the polar vitamins, the content of acetonitrile in the eluent was gradually increased up to 40% within 20 minutes, then, within 2 minutes, up to 100%, enabling elution of the less polar vitamins within another 27 minutes.

Both
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
3. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
4. A Unique Dimension in Biological Purity
5. Unique Histochemical Substrate Permits Dual Staining In Animal Tissue
6. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
7. Improve Recovery and Reproducibility with Pre-Treatment of Analytical TSK-GEL G3000SWXL Size Exclusion Columns
8. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
9. High-Fidelity PCR with a Novel Polymerase Mixture
10. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
11. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
Post Your Comments:
(Date:7/10/2014)... for crude 3-D glasses, polarized glasses, and shutter ... devices, used to trick the brain into perceiving ... obsolete with the introduction of new holography technology ... doctoral students Yuval Yifat, Michal Eitan, and Zeev ... nanoantennas that could be used for security as ...
(Date:1/15/2014)... an approach akin to assembling a club sandwich ... Technology (NIST) researchers have succeeded in crafting a ... flammability of foam commonly used in upholstered furniture ... the nanotube-coated polyurethane foam was reduced 35 percent ...
(Date:1/15/2014)... 2014 The Pittcon Organizing Committee is pleased ... Safety Tech , an e-Journal and producer of ... the second year for the co-location of Food Labs Conference ... provides that the registration fee to attend the two-day Food ...
(Date:1/15/2014)... January 15, 2014 Cynvenio, a cancer ... through the genomic analysis of tumor cells in the ... a web-based cancer decision support application powered by CollabRx, ... Cynvenio’s ClearID™ genomic test, Therapy Finder will provide breast ...
Breaking Biology Technology:Projecting a 3-dimensional future 2Projecting a 3-dimensional future 3Layered security: Carbon nanotubes promise improved flame-resistant coating 2Pittcon Announces Second Year Partnership With Food Safety Tech 2Pittcon Announces Second Year Partnership With Food Safety Tech 3Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 3
... , , , PLG can be used in small and large scale protocols ... given here works with E. coli cultures grown for 1214 , hours in ... ml Terrific Broth. , , , ... at room , temperature. , ...
... Isolation of Coxiella Burnetii Genomic DNA from Goat Placenta, Mouse ... Kit , , , , , , ... INRA centre of TOURS, Unit de Pathologie Infectieuse et Immunologie, , ... bouvery@tours.inra.fr , , , , , , ...
... , , , , , , , ... href="http://www.biocompare.com/techart.asp?id=575">Actinobacillus , ... href="http://www.biocompare.com/techart.asp?id=576">Actinomyces , ... href="http://www.brinkmann.com/pdf/ecet-protocols/4308915-501.pdf"> , <A ...
Cached Biology Technology:Basic Plasmid DNA Isolation Protocol 2EppendorfPerfect gDNA Blood Mini Kit 2EppendorfPerfect gDNA Blood Mini Kit 3EppendorfPerfect gDNA Blood Mini Kit 4EppendorfPerfect gDNA Blood Mini Kit 5Eppendorf Multiporator Transfection Protocols for Eukaryotic , Cells 2Eppendorf Multiporator Transfection Protocols for Eukaryotic , Cells 3Eppendorf Multiporator Transfection Protocols for Eukaryotic , Cells 4Eppendorf Multiporator Transfection Protocols for Eukaryotic , Cells 5Eppendorf Multiporator Transfection Protocols for Eukaryotic , Cells 6Eppendorf Multiporator Transfection Protocols for Eukaryotic , Cells 7
(Date:7/10/2014)... -- According to the new market research ... Material (Optical Prism, Pizeoelectric, Capacitive, and Adhesives), Application (Mobile ... Finance), and Geography - Global Trends & Forecasts to ... Sensors Market is projected to cross $14.35 Billion by ... 2014 to 2020. Browse more than 78 ...
(Date:7/10/2014)... 7, 2014 Fingerprint Cards, (FPC,s) new ... win (DW). A Chinese Top 5 smartphone OEM has selected ... mass production in October 2014.   FPC is ... smartphone OEM, which has a planned date for the start ... volume for this phone of 3 million units. The Chinese ...
(Date:7/10/2014)... , July 8, 2014 ... a new design win (DW). An Asian OEM has selected ... mass production start in August 2014.   FPC ... OEM, which has a planned date for start of mass ... ramp order of SEK 5M, for delivery in the first ...
Breaking Biology News(10 mins):Fingerprint Sensors Market worth $14.35 Billion by 2020 2Fingerprint Sensors Market worth $14.35 Billion by 2020 3FPC Wins DW for 1021 Touch Sensor for Flagship Smartphone from Chinese Top 5 Smartphone OEM 2FPC Wins DW and Initial Ramp Order for FPC1021 Touch Sensor for Flagship Smartphone from Asian OEM 2
... natural latexes are the main ingredient in the extraction ... kinds of industries and essential for the manufacture of ... in Europe is imported, extracted fundamentally from the the ... the world are Malaysia, Indonesia and Thailand, three Asian ...
... The U.S. Department of Agriculture,s National Agricultural Statistics ... images depicting agricultural land cover across most of the ... to as cropland data layers (CDL), are a useful ... water resources and carbon emissions. These crop-specific, digital ...
... of February 4th is only one week away! ... important international osteoporosis meeting of the year, the ... online:, http://www.iofwco-ecceo10.org/abstracts/ Abstracts will ... International, with opportunity for poster presentation. Authors of ...
Cached Biology News:Europe seeks alternatives to natural latex from Asia 2Europe seeks alternatives to natural latex from Asia 3
... Rabbit Antibody to ARG. Vasopressin ... vasopression peptide. Cells are found in ... fibers are found throughout the hypothalmus ... perfused tissue. Immunogen: ...
Mouse monoclonal [RL13] to LAP1 ( Abpromise for all tested applications). SwissProtID: Q96NW7...
Rabbit polyclonal to LCAT ( Abpromise for all tested applications). entrezGeneID: 3931 SwissProtID: P04180...
Sheep polyclonal to Malate Dehydrogenase ( Abpromise for all tested applications). Antigen: Full length protein - Pig heart malate dehydrogenase. Entrez Gene ID: 4190 Swiss Protein ID: P...
Biology Products: